Plan apochromatic

Manufactured by Nikon

Sourced in Japan

The Plan-Apochromatic is a high-quality optical lens system designed for laboratory and scientific applications. It features a multi-element, apochromatic lens design that provides superior color correction and flat field imaging. The Plan-Apochromatic lens is optimized for producing sharp, high-contrast images with minimal aberrations across a wide field of view.

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Lab products found in correlation

Plan fluor oil immersion objective lens, by Nikon (3 mentions) Csu x1 confocal unit, by Yokogawa (3 mentions) Te2000 microscope, by Nikon (3 mentions) Orca er ccd camera, by Hamamatsu Photonics (2 mentions) Ixon3 electron multiplier charge coupled device camera, by Oxford Instruments (2 mentions) No 1.5 coverglass, by MatTek (1 mentions) Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions) Ixon emccd camera, by Oxford Instruments (1 mentions) Csu x1 spinning disk confocal head, by Yokogawa (1 mentions) Lcv110 microscope, by Olympus (1 mentions) Phenol red free imdm, by Thermo Fisher Scientific (1 mentions) 1.4na oil immersion objective lens, by Nikon (1 mentions)

4 protocols using plan apochromatic

Microscopic Imaging of Cellular Dynamics

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Cells were observed under a TE2000 microscope (Nikon) equipped with a × 100 1.4 numerical aperture (NA) Plan-Apochromatic, a × 60 1.4 NA Plan-Apochromatic, or a × 40 1.3 NA Plan Fluor oil immersion objective lens (Nikon), a CSU-X1 confocal unit (Yokogawa), and an iXon3 electron multiplier-charge coupled device (EMCCD) camera (Andor) or an ORCA-ER CCD camera (Hamamatsu Photonics). Image acquisition was controlled by µManager software (Open Imaging).

Yaguchi K., Yamamoto T., Matsui R., Shimada M., Shibanuma A., Kamimura K., Koda T, & Uehara R. (2018). Tetraploidy-associated centrosome overduplication in mouse early embryos. Communicative & Integrative Biology, 11(4), e1526605.

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Live-cell imaging of mitotic spindles

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For phenotypic analysis of SOGA proteins knockdown by RNAi transfection, HeLa H2B-GFP/mCherry-α-tubulin cells were cultured in 35 mm glass-bottom dishes (14 mm, No 1.5 coverglass; MatTek Corporation). Cell culture medium was replaced with Leibovitz's-L15 medium (GIBCO, Life Technologies) supplemented with 10% FBS. Time-lapse imaging was performed in a heated chamber (37°C) using a 100× 1.4 NA Plan-Apochromatic differential interference contrast objective mounted on an inverted microscope (TE2000U; Nikon) equipped with a CSU-X1 spinning-disk confocal head (Yokogawa Corporation of America) and with two laser lines (488 nm and 561 nm). Images were acquired with an iXon+ EM-CCD camera (Andor Technology). Eleven 1-μm-separated z-planes covering the entire volume of the mitotic spindle were collected every 2 min.

Ferreira L.T., Logarinho E., Macedo J.C., Maia A.R, & Maiato H. (2021). SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for faithful chromosome segregation in human cells. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 29(2), 159-173.

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Live Cell Imaging of Cell Cycle

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Fixed or living cells were observed at 25°C or 37°C with 5% CO₂, respectively, under a TE2000 microscope (Nikon) equipped with a 100× 1.4 NA Plan-Apochromatic, a 60× 1.4 NA Plan-Apochromatic, or a 40× 1.3 NA Plan Fluor oil immersion objective lens (Nikon), a CSU-X1 confocal unit (Yokogawa), and an iXon3 electron multiplier-charge–coupled device camera (Andor) or an ORCA-ER CCD camera (Hamamatsu Photonics). Image acquisition was controlled by µManager software (Open Imaging). Long-term live imaging for cell cycle analyses was performed using a LCV110 microscope (Olympus) equipped with a 40× 0.95 NA UPLSAPO dry lens (Olympus). Because we found that 488-nm light irradiation severely interfered the progression of cell cycle and mitosis in HAP1 cells, we used bright-field microscope for long-term live imaging for cell cycle analyses (Figs. 1, 8, and S3). For live imaging, cells were cultured in phenol red–free IMDM (Thermo Fisher Scientific) supplemented with 10% FBS and 1× AA.

Yaguchi K., Yamamoto T., Matsui R., Tsukada Y., Shibanuma A., Kamimura K., Koda T, & Uehara R. (2018). Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals. The Journal of Cell Biology, 217(7), 2463-2483.

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Live-cell and Electron Microscopy Imaging

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The fixed and living cells were observed under a TE2000 microscope (Nikon, Tokyo, Japan) equipped with a 100×/1.4 numerical aperture (NA) Plan-Apochromatic, a 60×/1.4 NA Plan-Apochromatic, or a 40×/1.3 NA Plan Fluor oil immersion objective lens (Nikon), a CSU-X1 confocal unit (Yokogawa, Tokyo, Japan), and an iXon3 electron-multiplier charge coupled device camera (Andor, Belfast, United Kingdom). Image acquisition was controlled by μManager software. Fluorescence recovery after photobleaching was performed using a C2 (Nikon) confocal microscope equipped with a 60×/1.4 NA oil immersion objective lens (Nikon). TEM was performed as previously described (Uehara et al., 2013 (link)).

Uehara R., Kamasaki T., Hiruma S., Poser I., Yoda K., Yajima J., Gerlich D.W, & Goshima G. (2016). Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission. Molecular Biology of the Cell, 27(5), 812-827.

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