The human HCC cell line MHCC97L, which was tagged with a luciferase reporter gene, was gifted by Professor Man Kwan from the Department of Surgery, The University of Hong Kong. The HLF cell line was purchased from Japanese Collection of Research Bioresources cell bank. Both cell lines were cultured in Dulbecco's modified Eagle's medium (Gibco, Waltham, MA, USA) supplemented with 4.5g/L glucose, 10% foetal bovine serum (Gibco, MA, USA) and 1% penicillin/streptomycin (Gibco, MA, USA)); and they were kept in a 37 °C humidified incubator (Thermo Fisher Scientific Inc., Waltham, MA, USA) under 5% CO 2 . The DNA plasmid encoding pBABE-puro LEF1 was gifted by Joan Massague (plasmid #27023; Addgene Inc., Cambridge, MA); and the DNA plasmid expressing pAd/WT1-IRES-nAmCyan was gifted by Edward McCabe (plasmid #29756, Addgene). Mangiferin was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The stock solution of Mangiferin (100 mg/mL) was prepared in DMSO (Sigma-Aldrich Co., St. Louis, M.O., USA) and dH 2 O (1:9, v/v). For animal administration, Mangiferin (50 mg/kg) was prepared in 0.4% of sodium carboxyl methyl cellulose (Sigma-Aldrich Co., St. Louis, M.O., USA).
Mangiferin
Manufactured by Merck Group
Sourced in United States, Germany, Italy, India, Sao Tome and Principe
Mangiferin is a lab equipment product manufactured by Merck Group. It is a naturally occurring polyphenolic compound with antioxidant properties. The core function of Mangiferin is to serve as a reference standard for analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (12 mentions)
Quercetin, by Merck Group (10 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (10 mentions)
Caffeic acid, by Merck Group (6 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Citric acid, by Merck Group (4 mentions)
Ellagic acid, by Merck Group (4 mentions)
Hesperidin, by Merck Group (4 mentions)
Ascorbic acid, by Merck Group (4 mentions)
β actin, by Santa Cruz Biotechnology (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (3 mentions)
Catechin, by Merck Group (3 mentions)
Vanillic acid, by Merck Group (3 mentions)
Kaempferol, by Merck Group (3 mentions)
P coumaric acid, by Merck Group (3 mentions)
Apigenin, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Acetic acid, by Merck Group (3 mentions)
Protocatechuic acid, by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
66 protocols using mangiferin
1
Establishment and Characterization of Luciferase-Tagged Human Liver Cancer Cell Lines
2
ARPE-19 Cell Oxidative Stress Response
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ARPE-19 cells (CRL-2302™) were cultured in Dulbecco’s Modified Eagle’s Medium/F-12 medium containing 10% fetal bovine serum as previously described (Park et al., 2022 (link)). Mangiferin and H2O2 (Sigma-Aldrich, St. Louis, MO, USA) were dissolved in dimethyl sulfoxide (Thermo Fisher Scientific, Waltham, MA, USA) to prepare stock solutions, diluted to appropriate concentrations in culture medium, and then treated with cells. To trigger oxidative stress, cells were cultured in medium containing H2O2, and Mangiferin and/or zinc protoporphyrin IX (ZnPP, Sigma-Aldrich) were added 1 h before H2O2 exposure.
3
Mangiferin Treatment for Peri-Implantitis in Mice
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Upon ligature placement, mice in the mangiferin-treated peri-implantitis group were orally given mangiferin (Sigma-Aldrich Co., St Louis, MO, USA) once a day for 6 weeks (dose: 50 mg/kg bodyweight). Saline was used as vehicle, and mice in normal control and vehicle-treated peri-implantitis groups were given only saline. All animals received oral administration of mangiferin or saline from the day of ligature placement and received the last administration the day before they were sacrificed by CO2 inhalation. No mice died and all implants existed until the end of the study.
4
Preparation and Characterization of Mangiferin-Loaded Nanoparticles
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Mangiferin (MG), the copolymer poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) poloxamer 407 (Pluronic F127, F127) (PEO98-POP67-PEO98), isopropyl palmitate, acid yellow 25, Coomassie brilliant blue G 250, and oil red O were purchased from Sigma-Aldrich (St Louis, MO, USA). The soybean lecithin (PC) (90% phosphatidylcholine) was Epikuron 200 from Lucas Meyer (Hamburg, Germany). Polytetrafluoroethylene membranes (2 cm diameter, pore size 0.2 μm) were purchased from Merck (Milan, Italy). The solvents were of HPLC grade, and all other chemicals were of analytical grade.
5
Phytochemical Analysis of Potato and Mango Peel
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Potatoes (Solanum tuberosum L.) were bought from a local shop in Bangkok, Thailand. Mango (Mangifera indica L. cv. Chok Anan) peel was obtained from the Peace Canning (1958) Co. Ltd., Chiangmai, Thailand. We obtained Folin-Ciocalteu phenol reagent from Merck (Darmstadt, Germany). Triton X-100 and l -ascorbic acid were bought from Fluka (Steinheim, Germany). Sodium carbonate, polyvinylpyrrolidone, catechol, citric acid, sodium disulfite, gallic and protocatechuic acids used as a phenolic acid standard were obtained from Sigma-Aldrich, Merck, Steinheim, Germany. Mangiferin and ellagic acid were obtained from Sigma-Aldrich, Merck, Buchs, Switzerland. Acetic acid, and methanol were obtained from Merck (Darmstadt, Germany). Acetonitrile was obtained from Mallinckrodt (Phillipsburg, NJ, USA) and used in HPLC analyses.
6
Mangiferin and MTT Anticancer Protocol
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Mangiferin and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphnyl-2H-tetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO) and dissolved in DMSO (vehicle). Antibodies against p65, p50, MMP-9, nucleolin, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Roswell Park Memorial Institute Medium (RPMI), antibiotics mixture, and fetal bovine serum (FBS) were obtained from WelGENE Inc. (Daegu, Republic of Korea). Peroxidase-labeled goat anti-rabbit immunoglobulin was purchased from KOMA Biotechnology (Seoul, Republic of Korea). MMP-9 inhibitor I was obtained from Merck (Darmstadt, Germany). Other chemicals were purchased as Sigma grades.
7
Neuronal Culture Protocols and Reagents
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Neurobasal medium, B27 supplement, foetal bovine serum, horse serum, and other culture reagents were from Gibco (Invitrogen, Barcelona, Spain). Receptor antagonists MK801 was obtained from Tocris (Cookson, Bristol, UK). Morin and mangiferin (Mng) were obtained by Sigma (Sigma, St. Louis, MO, USA).
8
Anti-platelet Aggregation Activity of Mango
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The anti-platelet aggregation activity of mango and its by-products was evaluated by a turbidimetric method [14 (link)] using a lumi-aggregometer (Chrono-Log, Haverton, PA, USA). The PRP (200 × 109 platelets/L) was preincubated with 20 μL of phosphate-buffered saline, PBS, (negative control: maximum aggregation), or power extracts from each part of the mango (1 mg/mL) for 3 min at 37 °C. In order to evaluate the dose-dependent effects against platelet aggregation, different concentrations (0.1, 0.5, and 1 mg/mL) of the more active extract were tested as well as the antiplatelet properties of some standards compounds such as mangiferin (Sigma-Aldrich, St. Louis, Missouri, MO, USA) at 1 mg/mL. For all assays, platelet aggregation was induced by adding adenosine 5’-diphosphate (ADP, 4 μM) supplied by Sigma-Aldrich (St. Louis, Missouri, MO, USA) as an agonist. Platelet aggregation was measured by triplicate as the increase in light transmission occurred for 6 min and results were expressed as a percentage of inhibition of aggregation.
9
Development of Antioxidant-Loaded Lipid Nanocarriers
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Solid lipid Compritol 888 ATO (COMP, a mixture of mono-, di-, and triglycerides of behenic acid) was purchased from Gattefossè (Milan, Italy); oil Miglyol 812 (MIG, a mixture of medium-chain triglycerides) was obtained from Eigenmann & Veronelli S.p.A. (Milan, Italy) and Lutrol F68 (MW 8400 g/mol) was provided by BASF ChemTrade GmbH (Burgbernheim, Germany). Mangiferin (MGN, MW 422.33 g/mol), Trolox (MW 250.29 g/mol) and 2,2’-azobis(2-methylpropionamidine) dihydrochloride (AAPH, MW 271.19 g/mol) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein disodium salt (MW 332.31 g/mol) was purchased from Acros Organics (Milan, Italy). Triton X-100, propylene glycol and PBS commercial (10×) were purchased from Sigma-Aldrich (Madrid, Spain). Ultrapure water (18.2 Momega) was obtained by filtering through millipore (MilliQ®, Millipore Ibérica, Madrid, Spain).
10
Antioxidant Compound Extraction Protocol
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Mangiferin, hesperidin, and sodium azide were purchased from Sigma Chemical Co. (St. Louis, MO, USA). HPLC-grade methanol and ethanol were purchased from P.O.Ch. (Gliwice, Poland).
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