Cirrus hd oct model 5000

Automated 24–2 Humphrey perimetry (Zeiss HFA II-i series) and three-dimensional macular cube OCT (Zeiss Cirrus HD-OCT model 5000, 512 × 128 scan protocol with 6 × 6 × 2 mm volumes) were performed for each eye – right eye (OD) and left eye (OS). Peripapillary retinal nerve fiber layer thickness (pRNFL) was additionally obtained using an optic disc cube 200 × 200 protocol. Testing was performed three times over a year for patient AJ, and at a single time point for all control participants. Crawford and Howell's modified t-test was used to compare retinal thickness measurements and mean deviation for each eye at each time point for patient AJ to the control population (44 (link)). Intraocular pressure and fundus examination were also performed as part of the routine ophthalmologic assessment; results are reported in Supplementary Table 8.

OCTA images were acquired using Cirrus HD-OCT model 5000 (AngioPlex software, version 10.0; Carl Zeiss Meditec, Inc) in a dark room. All measurements were performed by one operator (M.L.) under FastTrac mode. For each eye, a 3 × 3 mm scan and a 6 × 6 mm scan centered on the fovea were acquired. Each scan was repeated at least twice for repeatability analysis. Automated OCT segmentation was performed on qualified images and manual adjustment was applied when segmentation error occurs. The qualifying images were defined satisfying the following requirements: (1) signal strength (SS) ≥7, (2) no more than one blink or motion artifact, and (3) the macular fovea remained in the center of the scanned area. The difference of SS between each two repeated scans should not lager than 1. Based on these default settings, the superficial capillary plexus (SCP) en face image was segmented with an inner boundary at internal limiting membrane (ILM) and an outer boundary at the junction of inner plexiform layer (IPL) and inner nuclear layer (INL) [9 (link)].

All subjects underwent a complete ophthalmic examination including best-corrected visual acuity, slip-lamp biomicroscopy, and dilated fundus examination. DR was confirmed by fundus photography (FFA Visucam NM/FA, Carl Zeiss, Germany) and optical coherence tomography imaging (Cirrus HD-OCT Model 5000, Carl Zeiss Meditec Inc., Dublin, CA, USA) and classified according to the ETDRS (Early Treatment of Diabetic Retinopathy Study, September 1, 2006) guidelines.

At each follow-up visit, patients underwent a complete ophthalmic examination, which included slit-lamp biomicroscopy, dilated fundoscopy, fundus photography, SD-OCT (Cirrus HD-OCT model 5000; Carl Zeiss Meditec, Inc., Dublin, Ireland), and UHR-SD-OCT (Bi-µ, Kowa). Another SD-OCT device (Heidelberg retina angiograph-OCT; Heidelberg Engineering, Heidelberg, Germany) was used only in cases where fluorescein angiography imaging was required. The central macular thickness (CMT) was measured using OCT as the average thickness of the central 1-mm thickness map measurement area.

All OCTA and OCT images were obtained using the same OCT device (Cirrus HD-OCT model 5000; Carl Zeiss Meditec, Dublin, CA). OCTA scanning protocols included 3 × 3 mm and 6 × 6 mm cube scans centered in the fovea by gaze fixation (Fig. 2). Structural OCT protocol included a macular cube scan (512 × 128). OCTA and OCT images with presence of artifacts, segmentation errors, or a signal strength index (SSI) < 7 were excluded from analysis. OCTA quantifications were performed by the device built-in commercial software (AngioPlex Metrix, Carl Zeiss Meditec, Dublin, CA) only in the SCP of the study eyes, defined as the layer between the internal limiting membrane (ILM) and the inner plexiform layer (IPL) boundaries. AngioPlex Metrix measurements included vessel and perfusion density and FAZ parameters (area, perimeter, and circularity) displayed for 3 × 3 mm and 6 × 6 mm scans. Vessel density is the total length of perfused vasculature per unit area in the region of measurement; perfusion density is the total area of perfused vasculature per unit area in the region of measurement. No manual adjustments of the segmentation slab boundaries were performed during the conduction of this study. A detailed description of OCTA images included and excluded from analysis is presented in Figure 1.

All participants completed automated 24–2 Humphrey perimetry testing for each eye (Zeiss HFAIIi, SITA standard perimetry using a size III white target, fixation enforced, corrected for near vision), as standard of care an average of 13 days post-stroke (range = 2–63 days). At the first study visit, participants received a full neuro-ophthalmologic exam. All study visits included Humphrey perimetry for each eye, spectral-domain macular OCT for each eye (Zeiss Cirrus HD-OCT model 5000, 512 × 128 scan protocol), and binocular fMRI retinotopic mapping (with the exception of participant 4 who did not complete fMRI at any time point, electronic supplementary material, table S1).