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Hla dr pc7

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The HLA-DR-PC7 is a fluorescent antibody that binds to the HLA-DR antigen expressed on the surface of antigen-presenting cells. It is designed for use in flow cytometry applications to identify and characterize these cell types.

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Lab products found in correlation

Cd15 fitc, by Beckman Coulter (4 mentions) Cd14 v450, by BD (3 mentions) Cd11b pe, by Beckman Coulter (3 mentions) Cd33 apc, by Beckman Coulter (3 mentions) Lsr 2 flow cytometer, by BD (2 mentions) Cd11b apc, by Beckman Coulter (1 mentions) Cd33 pe, by Beckman Coulter (1 mentions) Cd14 pacific blue, by Beckman Coulter (1 mentions) Cd8 apc h7, by BD (1 mentions) Cd4 pacific blue, by BD (1 mentions) Lsrii instrument, by BD (1 mentions) Cd45ra ecd, by Beckman Coulter (1 mentions) Versalyse, by Beckman Coulter (1 mentions) Ccr6 pe, by BD (1 mentions) Cd45 pacific orange, by Thermo Fisher Scientific (1 mentions) Cd3 af700, by BD (1 mentions) Icos apc, by BioLegend (1 mentions) Cd16 fitc, by Beckman Coulter (1 mentions)

Close spelling variants of this product

HLA-DR (15 citations) Anti-HLA-DR-PC7 (4 citations)

5 protocols using hla dr pc7

1

MDSC Characterization by Flow Cytometry

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MDSCs were obtained as described above. Cells were incubated with fluorochrome-labeled antibodies at 4°C. Specific antibodies include CD33 PE, HLA-DR PC7, CD11b APC, CD14 Pacific Blue and CD15 FITC (Beckman Coulter, Brea, CA). Flow cytometry was performed as previously described (20 (link)).
del Campo S.E., Levine K.M., Mundy-Bosse B.L., Grignol V.P., Fairchild E.T., Campbell A.R., Trikha P., Mace T.A., Paul B.K., Jaime-Ramirez A.C., Markowitz J., Kondadasula S.V., Guenterberg K.D., McClory S., Karpa V.I., Pan X., Olencki T.E., Monk J.P., Mortazavi A., Tridandapani S., Lesinski G.B., Byrd J.C., Caligiuri M.A., Shah M.H, & Carson WE I.I.I. (2015). The Raf Kinase Inhibitor Sorafenib Inhibits JAK-STAT Signal Transduction in Human Immune Cells. Journal of immunology (Baltimore, Md. : 1950), 195(5), 1995-2005.
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2

Quantifying Myeloid-Derived Suppressor Cells

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PBMC from each patient at the pre-treatment and post-Cycle 1 time points were analyzed for myeloid derived suppressor cells (MDSC) as previously described [25 (link)]. Specific antibodies included CD15-FITC (Beckman Coulter), CD33-APC (Beckman Coulter), HLA-DR-PC7 (Beckman Coulter), CD11b-PE (Beckman Coulter), and CD14-V450 (BD Biosciences). Single color staining was performed for compensation. All samples were run on a BD LSR II flow cytometer and were subsequently analyzed with FlowJo software (TreeStar). MDSC were defined as cells positive for CD33 and CD11b and lacking HLA-DR with subsets expressing CD15 or CD14 representing granulocytic and monocytic MDSC, respectively, as discussed in figure legends.
Duggan M.C., Jochems C., Donahue R.N., Richards J., Karpa V., Foust E., Paul B., Brooks T., Tridandapani S., Olencki T., Pan X., Lesinski G.B., Schlom J, & Carson WE I.I.I. (2016). A phase I study of recombinant (r) Vaccinia-CEA(6D)-TRICOM and rFowlpox-CEA(6D)-TRICOM vaccines with GM-CSF and IFN-α-2b in patients with CEA expressing carcinomas. Cancer immunology, immunotherapy : CII, 65(11), 1353-1364.
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3

Multiparameter flow cytometric analysis

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For the analysis of OX40L expression, whole blood samples were stained with anti-CD14-PC5, CD16-FITC, CD11c-APC, HLA-DR-PC7, and OX40L-PE mAbs, and red blood cells were lysed with Versalyse (Beckman Coulter). For the analysis of blood Tfh cells, whole blood samples were stained with anti-CXCR5-AF488, CCR6-PE, CXCR3-PC5, CCR4-PC7, CD3-AF700, CD8-APCH7, CD4-Pacific Blue (all from Becton Dickinson), CD45RA-ECD (Beckman Coulter), ICOS-APC (Biolegend) and CD45-Pacific Orange (Invitrogen). Data were collected using a BD LSR II instrument (BD Biosciences) and analyzed with Flowjo software (Tree Star Inc.).
Jacquemin C., Schmitt N., Contin-Bordes C., Liu Y., Narayanan P., Seneschal J., Maurouard T., Dougall D., Davizon E.S., Dumortier H., Douchet I., Raffray L., Richez C., Lazaro E., Duffau P., Truchetet M.E., Khoryati L., Mercié P., Couzi L., Merville P., Schaeverbeke T., Viallard J.F., Pellegrin J.L., Moreau J.F., Muller S., Zurawski S., Coffman R.L., Pascual V., Ueno H, & Blanco P. (2015). OX40 Ligand Contributes to Human Lupus Pathogenesis by Promoting T follicular Helper Response. Immunity, 42(6), 1159-1170.
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4

MDSC Phenotyping in PBMC

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PBMC were analyzed for the presence of MDSC as previously described [21 (link)]. MDSC were defined as cells positive for CD33, CD11b and lacking HLA-DR with subsets expressing CD15 or CD14 representing granulocytic and monocytic MDSC, respectively (Fig. 1). Notably, the current method for phenotyping M-MDSC (CD33+/HLADR-/CD14+/CD11b+) compares favorably to methods employed by other investigators (e.g., CD14+/HLADRlow/−) with respect to the percentages of M-MDSC obtained [22 (link)]. Specific antibodies included CD15-FITC, CD33-APC, HLA-DR-PC7, CD11b-PE (all Beckman Coulter), and CD14-V450 (BD Biosciences). All samples were run on a BD LSR-II flow cytometer and data was analyzed with FlowJo software (Tree Star, Inc.).
Wesolowski R., Duggan M.C., Stiff A., Markowitz J., Trikha P., Levine K.M., Schoenfield L., Abdel-Rasoul M., Layman R., Ramaswamy B., Macrae E.R., Lustberg M.B., Reinbolt R.E., Mrozek E., Byrd J.C., Caligiuri M.A., Mace T.A, & Carson WE I.I.I. (2017). Circulating myeloid derived suppressor cells increase in patients undergoing neo-adjuvant chemotherapy for breast cancer. Cancer immunology, immunotherapy : CII, 66(11), 1437-1447.
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5

Phenotypic Characterization of MDSC

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PBMC were analyzed for the presence of MDSC as previously described (12 (link)). Briefly, MDSC were defined as cells positive for CD33, CD11b and with low to no expression of HLA-DR with subsets expressing CD15 or CD14 representing granulocytic and monocytic MDSC, respectively (Figure 1). Specific antibodies included CD15-FITC, CD33-APC, HLA-DR-PC7, CD11b-PE (all Beckman Coulter), and CD14-V450 (BD Biosciences). All samples were analyzed on a BD LSR II flow cytometer and the data were subsequently analyzed with FlowJo software. Cells were then categorized into specific subsets: early MDSC (CD14-/CD15-), monocytic MDSC (CD14+/CD15+), granulocytic MDSC (CD14-/CD15+). Notably, there is a sizeable population within the gated cells that were double positive (CD14+/CD15+) that are not traditionally described as MDSC. As such, these cells were analyzed separately.
Sun S.H., Benner B., Savardekar H., Lapurga G., Good L., Abood D., Nagle E., Duggan M., Stiff A., DiVincenzo M.J., Suarez-Kelly L.P., Campbell A., Yu L., Wesolowski R., Howard H., Shah H., Kendra K, & Carson W.E. (2021). Effect of Immune Checkpoint Blockade on Myeloid-Derived Suppressor Cell Populations in Patients With Melanoma. Frontiers in Immunology, 12, 740890.
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