CBX8 reporter mESCs with 12xZFHD1, 4xGAL4 and 7xTETO DNA binding sites upstream of a Puromycin-GFP reporter gene were generated previously described dual reporter mESCs (Moussa et al., 2019 (link)) by recombinase mediated cassette exchange. mESCs were cultivated without feeders in high-glucose-DMEM (Sigma, D6429) supplemented with 13.5% fetal bovine serum (Gibco), 10 mM HEPES pH 7.4 (Corning, 25–060-CI), 2 mM GlutaMAX (Gibco, 35050–061), 1 mM Sodium Pyruvate (Gibco, 11360–070), 1% Penicillin/Streptomycin (Sigma, P0781), 1X non-essential amino acids (Gibco, 11140–050), 50 mM β-mercaptoethanol (Gibco, 21985–023) and recombinant LIF, and incubated at 37°C and 5% CO2. mESCs were passaged every 48 hours by trypsinization in 0.25% Trypsin-EDTA (1X) (Gibco, 25200–056) and seeding of 2.0 × 106 cells on a 10 cm tissue culture plate (Genesee Scientific, #25–202).
10 cm tissue culture plate
Manufactured by Genesee Scientific
The 10 cm tissue culture plate is a circular, flat-bottomed dish made of polystyrene material. It is designed for the cultivation and growth of cells, tissues, or organisms in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Merck Group (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Dmem high glucose, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Trypsin edta 1x, by Thermo Fisher Scientific (2 mentions)
Hepes ph 7, by Corning (2 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
2 protocols using 10 cm tissue culture plate
1
Dual Reporter Mouse Embryonic Stem Cells
2
Dual Reporter Mouse Embryonic Stem Cells
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CBX8 reporter mESCs with 12xZFHD1, 4xGAL4 and 7xTETO DNA binding sites upstream of a Puromycin-GFP reporter gene were generated previously described dual reporter mESCs (Moussa et al., 2019 (link)) by recombinase mediated cassette exchange. mESCs were cultivated without feeders in high-glucose-DMEM (Sigma, D6429) supplemented with 13.5% fetal bovine serum (Gibco), 10 mM HEPES pH 7.4 (Corning, 25–060-CI), 2 mM GlutaMAX (Gibco, 35050–061), 1 mM Sodium Pyruvate (Gibco, 11360–070), 1% Penicillin/Streptomycin (Sigma, P0781), 1X non-essential amino acids (Gibco, 11140–050), 50 mM β-mercaptoethanol (Gibco, 21985–023) and recombinant LIF, and incubated at 37°C and 5% CO2. mESCs were passaged every 48 hours by trypsinization in 0.25% Trypsin-EDTA (1X) (Gibco, 25200–056) and seeding of 2.0 × 106 cells on a 10 cm tissue culture plate (Genesee Scientific, #25–202).
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