Amicon ultra 0.5 ml 3k centrifugal filter units

Manufactured by Merck Group

Sourced in United States

Amicon Ultra-0.5 ml 3K centrifugal filter units are a type of laboratory equipment designed for sample concentration and buffer exchange. They feature a 3,000 molecular weight cutoff membrane and a 0.5 ml sample capacity.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Amicon Ultra 0.5 mL centrifugal units Amicon® Ultra-0.5 mL centrifugal Amicon Ultra-0.5 3k Amicon Ultra centrifugal units Amicon Ultra 0.5 mL Amicon centrifugal units 3K) Centrifugal Filters Amicon Ultra 3K Centrifugal filter units Amicon filter units

4 protocols using amicon ultra 0.5 ml 3k centrifugal filter units

Serotonin Release from i5HT Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

i5HT neurons cultured in six-well plates were incubated at 37 °C in 0.5 ml Hank's balanced salt solution (HBSS) for 30 min, or in 0.5 ml HBSS for 15 min and then 56 mM KCl was added for another 15 min, or in 0.5 ml HBSS without Ca²⁺ and without Mg²⁺, but with 2 mM EDTA for 15 min and then 56 mM KCl was added for another 15 min. The HBSS solutions were taken out from the wells and concentrated by Amicon ultra-0.5 ml 3k centrifugal filter units (Millipore, Billerica, MA, USA). The amounts of 5HT in HBSS solutions were measured by reverse phase HPLC (ESA Model 582 with ESA MD150 × 3.2 column, at 0.6 ml min⁻¹ flow rate in MD-TM mobile phase) coupled with electrochemical detection (ESA Coulochem III, E1: −250 mV, 2 μA; E2: 350 mV, 2 μA). Cells in the wells were lysed in 0.5 N NaOH to measure protein levels, which were used to normalize serotonin release.

Xu Z., Jiang H., Zhong P., Yan Z., Chen S, & Feng J. (2015). Direct conversion of human fibroblasts to induced serotonergic neurons. Molecular Psychiatry, 21(1), 62-70.

+ Open protocol

+ Expand

Serotonin Uptake Assay in i5HT Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

i5HT neurons cultured in six-well plates were rinsed with 1 ml prewarmed uptake buffer (10 mM HEPES, 130 mM NaCl, 1.3 mM KCl, 2.2 mM CaCl₂, 1.2 mM MgSO₄, 1.2 mM KH₂PO₄, 10 mM glucose, pH 7.4) three times. Cells were incubated for 5 min at 37 °C with 1 ml uptake buffer containing 5 μM serotonin without or with 10 μM citalopram. After the cells were washed at least three times in uptake buffer, they were lysed in 0.1 M perchloric acid with 1 mM EDTA and 0.1 mM sodium bisulfite. Cleared cell lysates were concentrated by Amicon ultra-0.5 ml 3k centrifugal filter units (Millipore) and analyzed for serotonin on HPLC coupled with electrochemical detection (E1: −250 mV, 2 μA; E2: 350 mV, 2 μA). The pellets of cellular proteins were dissolved in 0.5 N NaOH to measure protein contents, which were used to normalize serotonin uptake. The amount of endogenous serotonin in i5HT neurons without any treatment was also measured.

Xu Z., Jiang H., Zhong P., Yan Z., Chen S, & Feng J. (2015). Direct conversion of human fibroblasts to induced serotonergic neurons. Molecular Psychiatry, 21(1), 62-70.

+ Open protocol

+ Expand

Serotonin Uptake Assay in i5HT Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

i5HT neurons cultured in 6-well plates were rinsed with 1 ml prewarmed uptake buffer (10 mM HEPES, 130 mM NaCl, 1.3 mM KCl, 2.2 mM CaCl₂, 1.2 mM MgSO₄, 1.2 mM KH₂PO₄, 10 mM glucose, pH 7.4) three times. Cells were incubated for 5 min at 37°C with 1 ml uptake buffer containing 5 μM serotonin without or with 10 μM citalopram. After the cells were washed at least three times in uptake buffer, they were lysed in 0.1 M perchloric acid with 1 mM EDTA and 0.1 mM sodium bisulfite. Cleared cell lysates were concentrated by Amicon ultra−0.5ml 3k centrifugal filter units (Millipore) and analyzed for serotonin on HPLC coupled with electrochemical detection (E1: −250 mV, 2 μA; E2: 350 mV, 2 μA). The pellets of cellular proteins were dissolved in 0.5N NaOH to measure protein contents, which were used to normalize serotonin uptake. The amount of endogenous serotonin in i5HT neurons without any treatment was also measured.

Xu Z., Jiang H., Zhong P., Yan Z., Chen S, & Feng J. (2015). Direct conversion of human fibroblasts to induced serotonergic neurons. Molecular Psychiatry, 21(1), 62-70.

+ Open protocol

+ Expand

Serotonin Release Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

i5HT neurons cultured in 6-well plates were incubated at 37°C in 0.5 ml Hank’s Balanced Salt Solution (HBSS) for 30 min, or in 0.5 ml HBSS for 15 min and then 56 mM KCl was added for another 15 min, or in 0.5 ml HBSS without Ca²⁺ and without Mg²⁺, but with 2 mM EDTA for 15 min and then 56 mM KCl was added for another 15 min. The HBSS solutions were taken out from the wells and concentrated by Amicon ultra−0.5ml 3k centrifugal filter units (Millipore). The amounts of 5HT in HBSS solutions were measured by reverse phase HPLC (ESA Model 582 with ESA MD150×3.2 column, at 0.6 ml/min flow rate in MD-TM mobile phase) coupled with electrochemical detection (ESA Coulochem III, E1: −250 mV, 2 μA; E2: 350 mV, 2 μA). Cells in the wells were lysed in 0.5 N NaOH to measure protein levels, which were used to normalize serotonin release.

Xu Z., Jiang H., Zhong P., Yan Z., Chen S, & Feng J. (2015). Direct conversion of human fibroblasts to induced serotonergic neurons. Molecular Psychiatry, 21(1), 62-70.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!