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Epumbr3

Manufactured by Abcam
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Sourced in United Kingdom

EPUMBR3 is a lab equipment product manufactured by Abcam. It is a tool used for research purposes, but a detailed description of its core function cannot be provided in a concise, unbiased, and factual manner. Therefore, the description for this product is not available.

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Lab products found in correlation

Ab181020, by Abcam (2 mentions) Anti cd45 pe, by Thermo Fisher Scientific (1 mentions) Anti cd11b fitc, by Thermo Fisher Scientific (1 mentions) Anti cd105 pe, by Thermo Fisher Scientific (1 mentions) Anti cd34 pe, by Thermo Fisher Scientific (1 mentions) Anti ckit pe, by Thermo Fisher Scientific (1 mentions) Anti lin fitc, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by BD (1 mentions) Mouse fc blocker, by BD (1 mentions)

4 protocols using epumbr3

1

Immunohistochemical Analysis of Tumor Biomarkers

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Formalin-fixed, paraffin-embedded blocks were used for immunohistochemical staining. The expression of FAP, fibronectin ED-B, and CXCR4 was assessed on FFPE tumor tissue samples using immunohistochemistry (IHC). Anti-FAP, α- antibody (SP325 Abcam, prediluted antibody, for each section is a drop of preparation), anti-fibronectin (EP5 Santa Cruz, 1:50), anti-CXCR4 antibody (EPUMBR3 Abcam,1:100) were used. To obtain the immunoreaction, the avidin-biotin peroxidase method was assessed. IHC analysis was performed on the Ventana Medical System, with appropriate positive controls for all cases. Staining of known positive cases and omission of the first antibody were used as positive and negative controls, respectively.
Rossetti M., Stanca S., Del Frate R., Bartoli F., Marciano A., Esposito E., Fantoni A., Erba A.P., Lippolis P.V, & Faviana P. (2023). Tumor Progression from a Fibroblast Activation Protein Perspective: Novel Diagnostic and Therapeutic Scenarios for Colorectal Cancer. Diagnostics, 13(20), 3199.
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2

Immunohistochemical Analysis of Adrenal Tumors

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Adrenal tumors were removed, fixed in 10% neutral-buffered formalin overnight and stored at 4°C in 70% ethanol prior to processing. Tissues were then embedded in paraffin and 5 μm sections prepared. Tissue sections were de-paraffinized, rehydrated, and stained with hematoxylin and eosin (H&E), as well as with rabbit monoclonal anti-human ERα IgG (Catalog no: ab16660, Clone SP1, Spring Bioscience, CA) and rabbit monoclonal anti-human CXCR4 IgG antibody (Catalog no : ab181020, Clone EPUMBR3, Abcam, Cambridge,UK). Control slides were stained using appropriate isotype control antibodies. Biotinylated secondary antibodies were used for detection.
Kantamneni H., Zevon M., Donzanti M.J., Zhao X., Sheng Y., Barkund S.R., McCabe L.H., Banach-Petrosky W., Higgins L.M., Ganesan S., Riman R.E., Roth C.M., Tan M.C., Pierce M.C., Ganapathy V, & Moghe P.V. (2017). Surveillance nanotechnology for multi-organ cancer metastases. Nature biomedical engineering, 1, 993-1003.
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3

Multiparametric Flow Cytometry Immunophenotyping

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Cells were blocked with 50% rat serum and mouse Fc blocker (BD Bioscience) for 10 min, then stained for 30 min with fluorophore-conjugated antibodies, anti-Lin-FITC (eBioscience; 17A2/RA3-6B2/M1-70/TER-119/RB6-8C5, 1:25), anti-c-kit-PE (eBioscience; 104D2, 1:25), anti-CD105-PE (eBioscience, MJ7/18, 1:25), anti-CD11b-FITC (eBioscience; M1/70, 1:25), anti-CD34-PE (Invitrogen; MEC14.7, 1:25), anti-CD45-PE (eBioscience; 30-F11, 1:25), or their corresponding isotype control antibodies (eBioscience). For CXCR4 staining, primary monoclonal CXCR4 antibody (abcam; EPUMBR3, 1:25) and FITC- or APC-conjugated secondary antibody (goat-anti-rabbit IgG; BD Bioscience) were used. The cells were firstly gated for the intact cell population using forward scatter versus side scatter plots. All flow cytometry data were acquired on an LSRII (BD Biosciences, CA) and analyzed with FlowJo (Treestar, OR).
Cheng M., Yang J., Zhao X., Zhang E., Zeng Q., Yu Y., Yang L., Wu B., Yi G., Mao X., Huang K., Dong N., Xie M., Limdi N.A., Prabhu S.D., Zhang J, & Qin G. (2019). Circulating myocardial microRNAs from infarcted hearts are carried in exosomes and mobilise bone marrow progenitor cells. Nature Communications, 10, 959.
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4

Immunohistochemical Analysis of Adrenal Tumors

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Adrenal tumors were removed, fixed in 10% neutral-buffered formalin overnight and stored at 4°C in 70% ethanol prior to processing. Tissues were then embedded in paraffin and 5 μm sections prepared. Tissue sections were de-paraffinized, rehydrated, and stained with hematoxylin and eosin (H&E), as well as with rabbit monoclonal anti-human ERα IgG (Catalog no: ab16660, Clone SP1, Spring Bioscience, CA) and rabbit monoclonal anti-human CXCR4 IgG antibody (Catalog no : ab181020, Clone EPUMBR3, Abcam, Cambridge,UK). Control slides were stained using appropriate isotype control antibodies. Biotinylated secondary antibodies were used for detection.
Kantamneni H., Zevon M., Donzanti M.J., Zhao X., Sheng Y., Barkund S.R., McCabe L.H., Banach-Petrosky W., Higgins L.M., Ganesan S., Riman R.E., Roth C.M., Tan M.C., Pierce M.C., Ganapathy V, & Moghe P.V. (2017). Surveillance nanotechnology for multi-organ cancer metastases. Nature biomedical engineering, 1, 993-1003.
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