Antibody against ha

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Antibody against HA is a laboratory reagent used for the detection and analysis of proteins tagged with the hemagglutinin (HA) epitope. This antibody specifically binds to the HA tag, allowing for the identification and characterization of HA-tagged proteins in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Protein a g sepharose beads, by GE Healthcare (1 mentions) Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Antibody against idh1, by R&D Systems (1 mentions) Goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Beauvericin, by Merck Group (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Raw264, by American Type Culture Collection (1 mentions)

3 protocols using antibody against ha

Immunoprecipitation of HA-tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

R-H460 cells were transfected with vectors under the indicated experimental conditions for 48 h. The cells were washed twice with PBS, harvested, and lysed for 30 min in NP-40 buffer [50 mM Tris-HCl (pH 8), 150 mM NaCl, 1% NP-40, and 100X protease and phosphatase inhibitor cocktail]. Samples were diluted to 500 µg of protein in 800 µl of buffer and pre-cleared for 1 h at 4 °C with 50 µl of a 50% slurry of protein A/G-Sepharose beads (GE Healthcare). After brief centrifugation to remove pre-cleared beads, 1 µg of antibody against HA (Santa Cruz Biotechnology) was added to each sample and incubated on a rocking platform at 4 °C overnight. The immune complex was precipitated by incubation with 40 µl of protein A/G-Sepharose beads at 4 °C for 4 h. The beads were washed thrice with immunoprecipitation buffer and then boiled with sample buffer [0.1 M Tris-HCl (pH 6.8), 4% SDS, 40 mM EDTA, 20% glycerol, and β-mercaptoethanol].

Baek J.H., Yun H.S., Kwon G.T., Lee J., Kim J.Y., Jo Y., Cho J.M., Lee C.W., Song J.Y., Ahn J., Kim J.S., Kim E.H, & Hwang S.G. (2019). PLOD3 suppression exerts an anti-tumor effect on human lung cancer cells by modulating the PKC-delta signaling pathway. Cell Death & Disease, 10(3), 156.

+ Open protocol

+ Expand

Antibody Characterization for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against DYKDDDDK (FLAG) tag, p-Tyrosine (p-Tyr-100), Jak2, p-Jak2 (Tyr1007/1008), di-methyl-histone H3 (Lys9), histone H3, FLT3 and Src were from Cell Signaling Technology (CST). Antibodies against β-actin and p-Src (Tyr418) were from Sigma-Aldrich. Antibody against HA was from Santa Cruz Biotechnology. Antibody against trimethyl (Lys9) was from Abcam. Antibodies against IDH1-R132H and Trimethyl-Histone H3 (Lys4) were from EMD Millipore. Antibody against IDH1 was from R&D SYSTEMS. Antibody against PE-Cy^™5 Mouse Anti-Human CD235a was from BD Pharmingen^™. Goat anti-Mouse IgG (H+L) secondary antibody and goat anti-rabbit IgG (H+L) secondary antibody were from Thermo Fisher Scientific. Antibodies against p-IDH1 Y42 and p-IDH1 Y391 were custom-made by SHANGHAI GENOMICS, INC. Antibodies against Ki67 and IDH1 for IHC staining studies were from Abcam.

Chen D., Xia S., Wang M., Lin R., Li Y., Mao H., Aguiar M., Famulare C.A., Shih A.H., Brennan C.W., Gao X., Pan Y., Liu S., Fan J., Jin L., Song L., Zhou A., Mukherjee J., Pieper R.O., Mishra A., Peng J., Arellano M., Blum W.G., Lonial S., Boggon T.J., Levine R.L, & Chen J. (2019). Mutant and wild-type isocitrate dehydrogenase 1 share enhancing mechanisms involving distinct tyrosine kinase cascades in cancer. Cancer discovery, 9(6), 756-777.

+ Open protocol

+ Expand

Investigating Anti-inflammatory Mechanisms

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Beauvericin (purity: 97%), 5-diphenyltetrazolium bromide (MTT), and lipopolysaccharide (LPS; E. coli 0111:B4) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). A luciferase construct that contained promoters sensitive to NF-κB was used as reported previously [20 (link)]. Fetal bovine serum (FBS), antibiotics (penicillin and streptomycin), phosphate-buffered saline (PBS), DMEM, RPMI 1640, and trypsin were obtained from Gibco (Grand Island, NY, USA). The murine macrophage cell line RAW264.7 and human embryonic kidney cells were purchased from the American Type Culture Collection (Rockville, CA, USA). PP2 and piceatannol were obtained from Calbiochem (La Jolla, CA, USA). Hoechst staining solution and antibodies (phospho-specific and/or total protein) against p65, p50, lamin A/C, p85, AKT, inhibitor of κBα (IκBα), Src, Syk, Myc, and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against HA was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rhodamine phalloidin was purchased from Life Technologies (Waltham, MA, USA).

Yoo S., Kim M.Y, & Cho J.Y. (2017). Beauvericin, a cyclic peptide, inhibits inflammatory responses in macrophages by inhibiting the NF-κB pathway. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 21(4), 449-456.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!