A modified Eagle’s minimum essential medium (mEMEM) was prepared using the following components (per L): 10 mL 100X non-essential amino acids (Sigma M7145), 20 mL 50X essential amino acids (Sigma 5550), 10 mL 100X vitamin mix (Thermo Fisher 1112052), 10 mL 100X sodium pyruvate (Sigma 5280), 10 mL 100X phenol red sodium salt (Sigma 3532), 292 mg L-glutamine, 200 mg CaCl2, 97 mg MgSO4, 400 mg KCl, 1.5 g NaHCO3, 140 mg NaH2PO4·H2O, and 6.8 g NaCl. 12C D-glucose (Sigma) or 13C D-glucose (Cambridge Isotope Labs, CLM-1396–5) was added to a final concentration of 1.0g/L or 1.33 g/L, respectively. The medium was then vacuum-filtered through a 0.22-μm filter, followed by the addition of 10% FBS (Thermo), penicillin G (100 U/mL) and streptomycin (100 μg/mL), prior to storage at 4°C.
Phenol red sodium salt
Manufactured by Merck Group
Sourced in United States
Phenol red sodium salt is a pH indicator commonly used in biological and laboratory applications. It changes color in response to changes in pH, making it useful for monitoring and measuring pH levels in various solutions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Non essential amino acids (neaa), by Merck Group (1 mentions)
13c d glucose, by Cambridge Isotopes (1 mentions)
Potassium hydroxide, by Resonac (1 mentions)
Sodium acetate, by Vetec (1 mentions)
Dopamine hydrochloride da hcl, by Thermo Fisher Scientific (1 mentions)
2 protocols using phenol red sodium salt
1
Modified Eagle's Minimum Essential Medium
2
Synthesis and Characterization of Metal-Organic Compounds
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Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), PEI (branched, MW 800 Da), chloramine-T hydrate, and phenol red sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dopamine hydrochloride (DA-HCl) was purchased from Acros Organic (Geel, Belgium). Potassium hydroxide, hydrochloride, sodium thiosulfate, and potassium bromide were purchased from SHOWA (Gunma, Japan). Ethylenediamine (En), anhydrous cobalt chloride, silver nitrite, and anhydrous copper chloride were purchased from Alfa Aesar (Tewksbury, MA, USA). Sodium chloride was purchased from Fluka (Munich, Germany). Sodium acetate was purchased from Vetec (St. Louis, MO, USA). All these chemicals were of analytical grade and were used without further purification. The PPMM (catalog number: 201PP-47-045-50; diameter of 47 mm; pore size of 450 nm; thickness of 200 µm) was purchased from Rone Scientific Inc. (New Taipei City, Taiwan).
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