Unless otherwise stated, all reagents were of analytical grade and purchased from Sigma-Aldrich, Indonesia. α-MG was purchased from Aktin Chemicals, Inc., Chengdu, China (batch #AM-170622, and ≥98% purity with HPLC method), whereas streptozotocin (STZ) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA (catalog #sc-200719A)).
Streptozotocin (stz)
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Streptozotocin (STZ) is a chemical compound used in research applications. It is a cytotoxic agent that primarily targets and destroys insulin-producing beta cells in the pancreas. STZ is commonly used in animal models to induce experimental diabetes for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Donepezil hydrochloride, by Merck Group (2 mentions)
Streptozotocin (stz), by Merck Group (2 mentions)
Ethanol, by Daejung Chemicals (1 mentions)
Hplc grade methanol, by Duksan Pure Chemicals (1 mentions)
Melatonin mel, by Merck Group (1 mentions)
Metformin, by Santa Cruz Biotechnology (1 mentions)
Glibenclamide, by Santa Cruz Biotechnology (1 mentions)
Losartan potassium, by Santa Cruz Biotechnology (1 mentions)
Metformin hydrochloride, by Merck Group (1 mentions)
Thymoquinone, by Santa Cruz Biotechnology (1 mentions)
Roswell park memorial institute (rpmi), by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Peptone, by HiMedia (1 mentions)
Sabouraud dextrose agar, by HiMedia (1 mentions)
Yeast extract, by HiMedia (1 mentions)
Sodium citrate, by Merck Group (1 mentions)
Progesterone, by Tokyo Chemical Industry (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Glucagon, by Merck Group (1 mentions)
Sodium pyruvate, by Merck Group (1 mentions)
29 protocols using streptozotocin (stz)
1
Diabetic Rat Model Induction Protocol
2
Induction of Experimental Diabetes in Mice
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Diabetic mice were established by intraperitoneal injection of 100 mg/kg streptozotocin (STZ) (Santa Cruz Biotechnology, Inc.) dissolved in 5 mM sodium citrate buffer (pH 4.5) after one night starvation. After 14 days, the blood glucose levels were higher than 250 mg/dL, and the mice were considered to have diabetes.
3
Quantification of Rhynchophylline Alkaloids
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Donepezil hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO, USA). Streptozotocin (STZ, purity ≥ 98%) was purchased from Santa Cruz Biotechnology (Dallas, USA). Ethanol was obtained from DAEJUNG Chemicals (Gyeonggi-do, Korea). MEthanol (HPLC grade) was bought from Duksan Pure Chemicals (Gyeonggi-do, Korea). Triethylamine (HPLC grade) was purchased from Scharlau (Barcelona, Spain). Standard substances (rhynchophylline, isorhynchophylline, corynoxeine and isocorynoxeine, purity ≥ 98%) were purchased from Chengdu Mansite Pharmacetical Co. Ltd (Chengdu, China). All other chemicals and reagents used were of analytical grade.
4
Streptozotocin Solution Preparation
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Streptozotocin (STZ) (Santa Cruz Biotechnology, CA, USA) was purchased from Gamma Scientific Biolab Company. The STZ solution is prepared by dissolving in sodium citrate buffer 100 mM (pH 4.5).
5
Streptozotocin-Induced Diabetes Model
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Streptozotocin (STZ, Santa Cruz Biotechnology, Santa Cruz, CA, USA) 1 mg/kg and AZ1 formulation (Instanth® NEO, Prodiet Medical Nutrition, PR, Brazil). AZ1 formulation was administered by gavage (1 g/kg) once a day for 30 days and its composition is in Table 1 .
6
Streptozotocin and Melatonin in Neuroprotection
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The dose and hour of administration of melatonin (dose of 10 mg/kg) were based on previous studies that described its neuroprotective effects (Luengo et al., 2019 ; Rong et al., 2020 ; Omeiza et al., 2021 ). It was dissolved in propylene glycol and diluted in saline, resulting in a final ratio of 5:95 propylene glycol/saline. The final concentration of suspended melatonin was 10 mg/ml, which was infused intraperitoneally (i.p). The control groups received 1 ml/kg of a 5% propylene glycol saline solution.
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streptozotocin (STZ; Santa Cruz Biotechnology Inc,. Califórnia, EUA).
Melatonin (MEL; Sigma-Aldrich, St. Louis, MO, USA).
The dose and hour of administration of melatonin (dose of 10 mg/kg) were based on previous studies that described its neuroprotective effects (Luengo et al., 2019 ; Rong et al., 2020 ; Omeiza et al., 2021 ). It was dissolved in propylene glycol and diluted in saline, resulting in a final ratio of 5:95 propylene glycol/saline. The final concentration of suspended melatonin was 10 mg/ml, which was infused intraperitoneally (i.p). The control groups received 1 ml/kg of a 5% propylene glycol saline solution.
7
Streptozotocin-Induced Diabetic Model
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Streptozotocin (STZ) (sc-200719; Santa Cruz Biotechnology, Dallas, TX, USA), deoxycorticosterone acetate (DOCA) (sc-239659; Santa Cruz Biotechnology), losartan potassium (sc-204796A; Santa Cruz Biotechnology), metformin (sc-202000B; Santa Cruz Biotechnology), glibenclamide (sc-200982A; Santa Cruz Biotechnology) and sodium chloride (NaCl) (LOBA Chemie PVT Ltd, India) were used in this study.
8
Streptozotocin-Induced Diabetes Protocol
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Streptozotocin (STZ) (Cat no: SC-200719) was purchased from Santa Cruz Biotechnology (Aspire Biosains PLT, Malaysia). In the fasting condition, the animals were injected with STZ at 45 mg/kg/day (i.p) in 0.5 mL of 10 mM citrate buffer (pH 5.5) for three days continuously. The blood glucose was measured using a blood glucometer (Gluco Dr, South Korea) from the withdrawn tail blood vein. Animals were considered as hyperglycaemia or diabetic state if the blood glucose level at or more than 250 mg/dL (Gholamhosseinian et al. 2020 (link)).
9
Streptozotocin-Induced Oxidative Stress
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Streptozotocin (STZ) was acquired from Santa Cruz Biotechnology USA. The ELISA kit used to detect TNF-α was acquired from Bioassay Technology Laboratory China (No. E0764Ra). The Total SOD Activity (No. E-BC-K019-S) and MDA Colorimetric (No. E-BC-K025-S) Assay Kit were obtained from Elabscience Biotechnology China. The polyclonal antibodies against JNK (No. bs-2900R) and Caspase3 (No. bs-0081R) were acquired from Bioss USA.
10
Streptozotocin-Induced Diabetes Mellitus in Rats
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After acclimatization of rats with regular chow diet for one week and, streptozotocin (STZ) (Santa Cruz Biotechnology, Heidelberg-Germany) was dissolved in sodium citrate buffer (0.1 M, pH: 4.5) and a dose of 55 mg per kg of body weight was intraperitoneally injected to the rats to induce type 1 diabetes mellitus (T1DM) in diabetic groups (D, D + Cur50, D + Cur150, D + Met300, and D + Met500). Seventy-two hours later, the level of fasting blood sugar (FBS) was measured using a glucometer to confirm induction of diabetes. FBS level higher than 250 mg/dl was considered as diagnostic off point of diabetes.16 (link) After induction of diabetes (day zero; D0) treatment of the rats with curcumin or metformin was started and continued for 42 days.14 (link) Twenty-four hours after the last treatment, the rats were anesthetized with ketamine–xylazine (50mg/kg ketamine and 12mg/kg xylazine) and sacrificed. Blood samples were collected from jugular vein and sera were separated and stored at -20°C until analysis of biochemical parameters. Heart tissue samples were dissected and used to determine oxide-redox status and to evaluate gene expression levels. Portions of tissue samples were also fixed in 10% neutral-buffered formalin for histopathological evaluation.
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