NK cells were incubated as indicated with entinostat (1.0 μM), VPA (0.1 mM), RGFP966 (0, 2.5, 5.0, 10 and 20 uM) for 24 hrs and then were lysed with 50 mM Tris-Cl (pH 6.8), 100 mM dithiothreitol, 2% SDS, and 10% glycerol. Samples were quantitated using BCA protein reagent assay kit (Yeasen, Shanghai) and analyzed by 8~10% of SDS-PAGE, followed by immunoblotting using Enhanced Chemiluminescence Substrate (MerckMillipore, USA) according to the manufacturer’s instructions. Bands were visualized using a chemiluminescent detection system (ProteinSimple, USA).
Bca protein reagent assay kit
Manufactured by Yeasen
Sourced in China
The BCA protein reagent assay kit is a laboratory tool used to quantify the total protein concentration in a sample. It is a colorimetric detection method that relies on the reduction of copper ions by proteins in an alkaline medium, resulting in a purple-colored complex that can be measured spectrophotometrically. The kit provides the necessary reagents and protocols to perform this assay.
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Lab products found in correlation
3 protocols using bca protein reagent assay kit
1
Immunoblotting of NK cell proteins
2
Protein Expression Analysis in RAW264.7 Cells
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RAW264.7 cells were seeded into 6-well plates at a density of 5 × 105 per well, and treated with different concentrations of YPF for 24 h. Then the cells were lysed with RIPA lysis buffer, and quantitated using BCA protein reagent assay kit (YEASEN, China) and analyzed by 8% of SDS-PAGE, followed by immunoblotting using enhanced chemiluminescence substrate (Merck Millipore) according to the manufacturer’s instructions. Bands were visualized using a chemiluminescent detection system (Bio-Rad). The expression of β-actin protein was used as a standard.
3
Aila Dose-Dependent Protein Expression
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A549, H1299 and H1975 cells were respectively seeded in 10-cm plates and incubated with different concentrations (0, 0.625, 1.25, 2.5 and 5.0 μM ) of Aila for 24 h. The cells were then lysed with RIPA lysis buffer, and protein contents were quantitated using BCA protein reagent assay kit (Yeasen, Shanghai, China) and analysed by 8–10% of SDS–PAGE, followed by immunoblotting using enhanced chemiluminescence substrate (Merck Millipore) according to the manufacturer’s instructions. Bands were visualised using a chemiluminescent detection system (ProteinSimple, San Jose, CA, USA).
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