Triple toftm 5600 system ms ms

Manufactured by AB Sciex

Sourced in United States

The AB Sciex Triple TOFTM 5600 system-MS/MS is a high-resolution mass spectrometry instrument. It is capable of performing tandem mass spectrometry (MS/MS) analysis.

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Lab products found in correlation

Zorbax sb c18 column, by Agilent Technologies (3 mentions) Uflc system, by Shimadzu (2 mentions) Agilent 7890a 5975c gc ms, by Agilent Technologies (1 mentions) Drx 500 spectrometer, by Bruker (1 mentions)

Close spelling variants of this product

Triple TOF 5600+ MS/MS system Triple TOF 5600 System Triple TOFTM 5600+ system Triple TOFTM 5600

5 protocols using triple toftm 5600 system ms ms

UFLC-MS/MS Analysis of Compounds

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A UFLC system (Shimadzu, Kyoto, Japan) was used for sample analysis. The separation was conducted by an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) at 30 °C. The mobile phase consisted of methanol (A) and 0.1% formic acid in water (B) with the following gradient elution: 0–2 min (5–30% A), 2–25 min (30–55% A), 25–30 min (55–5% A) and 30–32 min (5–5% A). The column temperature was 30 ℃. The detection wavelength was 360 nm. The injection volume was 10 μL and the flow rate was 0.8 mL/min.
MS data were recorded using an AB Sciex Triple TOF^TM 5600 system-MS/MS (AB SCIEX, Framingham, MA, USA), equipped with an electrospray ionization (ESI) source. The optimized MS conditions in negative ion mode were set as follows: ion source temperature (TEM), 600 °C; flow rate of curtain gas (CUR), 40 psi; flow rate of nebulization gas (GS1) and flow rate of auxiliary gas (GS2), 60 psi; ion spray voltage floating (ISVF), 4500 V; collision energy, −10 V; declustering potential, −100 V. Data were acquired for each sample from 50 to 1500 Da. The data acquisition time was 32 min.

Zhou Y., Chen H., Xue J., Yuan J., Cai Z., Wu N., Zou L., Yin S., Yang W., Liu X., Chen J, & Liu F. (2022). Qualitative Analysis and Componential Differences of Chemical Constituents in Lysimachiae Herba from Different Habitats (Sichuan Basin) by UFLC-Triple TOF-MS/MS. Molecules, 27(14), 4600.

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Rapid LC-MS/MS Quantification Analysis

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An LC system (Shimadzu, Kyoto, Japan) was used for sample analysis. The separation was conducted by an ODS C18 analytical column (4.6 mm × 250 mm, 5 µm; GL sciences, Inc., Tokyo, Japan) at 30 °C. The mobile phase consisted of 0.1% formic acid in water (A) and methanol (B) with the following gradient elution: 0–5 min (10–14% B), 6–22 min (14–30% B), 22–27 min (30–52% B), 27–55 min (52–80% B), 55–65 min (80–100% B), and 65–72 min (100% B). The column temperature was 30 °C. The injection volume was 10 µL and the flow rate was 1 mL/min. MS data were recorded using an AB Sciex Triple TOF^TM 5600 system-MS/MS (AB SCIEX, Framingham, MA, USA) equipped with an electrospray ionization (ESI) source. The optimized MS conditions in positive-ion mode were set as follows: ion source temperature (TEM), 600 °C; flow rate of curtain gas (CUR), 40 psi; flow rate of nebulization gas (GS1) and flow rate of auxiliary gas (GS2), 60 psi; ion-spray voltage floating (ISVF), 5500 eV; collision energy (CE), 40 V. Data were acquired for each sample from 50 to 1500 Da. The data acquisition time was 72 min.

Qiu H., Shan C., Fei C., Xue P., Zhou Y., Yuan J, & Liu X. (2023). Geoherbalism Metabolomic Analysis of Atractylodes lancea (Thunb.) DC. by LC-Triple TOF-MS/MS and GC-MS. Molecules, 28(16), 5974.

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UFLC-MS/MS Analysis of Compounds

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The UFLC system (Shimadzu., Kyoto, Japan) was used for sample analysis. The separation was conducted by an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) at 30 °C. The mobile phase was composed of methanol-acetonitrile (1:1) (A) and 0.4% formic acid water (B) with the gradient elution: 0–5 min, 2–6% A; 5–6 min, 6–10% A; 6–8 min, 10–15% A; 8–12 min, 15–18% A; 12–18 min, 18–21% A; 18–21min, 21–23% A; 21–26 min, 23–25% A; 26–30 min, 25–27% A; 30–33 min, 27–40% A; 33–38 min, 40–50% A; 38–40 min, 50–2% A; 40–45 min, 2–2% A. The injection volume was 10 μL and the flow rate was 1.0 mL/min.
Mass spectrometry (MS) detection was performed by AB Sciex Triple TOF TM 5600 system-MS/MS (AB SCIEX, Framingham, MA, USA), equipped with an electrospray ionization (ESI) source in negative ion mode. The optimized MS conditions were set as follows: the ion source temperature (TEM): 600 °C; the flow rate of curtain gas (CUR): 40 psi; the flow rate of nebulization gas (GS1): 60 psi; the flow rate of auxiliary gas (GS2): 60 psi; the ion spray voltage floating (ISVF): 4500 V; the collision energy: −10 V; the declustering potential: −100 V. TOF MS and TOF MS/MS were scanned with the mass range of m/z 100~2000 and 50~1500, respectively.

Yuan J., Li L., Cai Z., Wu N., Chen C., Yin S., Liu S., Wang W., Mei Y., Wei L., Liu X., Zou L, & Chen H. (2021). Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS. Molecules, 26(21), 6373.

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NMR, MS, and Chromatography Methods

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NMR spectra were recorded on a Bruker DRX-500 spectrometer (Bruker, Karlsruhe, Germany), using CDCl₃ as solvents. ESI-MS data were obtained on an AB Sciex Triple TOF^TM 5600 system-MS/MS (AB SCIEX, Framingham, MA, USA) mass spectrometer. EI-MS data were obtained on an Agilent 7890A/5975C GC-MS (Agilent, J&W Scientific, Folsom, CA, USA) mass spectrometer. Column chromatography was performed on silica gel (100–200 mesh and 200–300 mesh, Qingdao Marine Chemical Factory, Qingdao, China). TLC was performed on precoated silica gel plates (G and GF254, Qingdao Marine Chemical Factory).

Qiu H., Shan C., Fei C., Xue P., Zhou Y., Yuan J, & Liu X. (2023). Geoherbalism Metabolomic Analysis of Atractylodes lancea (Thunb.) DC. by LC-Triple TOF-MS/MS and GC-MS. Molecules, 28(16), 5974.

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High-Resolution Liquid Chromatography-Mass Spectrometry

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The separation was performed using the Agilent ZORBAX SB C18 column (Agilent, Palo Alto, CA, USA). The mobile phase was composed of 0.1% aqueous formic acid (A) and acetonitrile with 0.1% formic acid (B) at the flow rate of 0.3 mL/min. 1 μL injection volume and column temperature 35°C. The gradient elution in the following separation program: 0–4 min (2% B); 4–5 min (2–10% B); 5–25 min (10–18% B); 25–29 min (18–25% B); 29–30 min (25–44% B); 30–33 min (44–48% B); 33–38 min (48–72% B); 38–45 min (72–95% B).
The Triple-TOF-MS was operated on AB SCIEX Triple TOF TM 5600 System-MS/MS (AB SCIEX, Framingham, MA, USA) equipped with an electronic spray ionization (ESI) source in positive and negative ion mode. The full scan mass range was set to m/z 100–2000 to acquire TOF-MS data, the scanning range of m/z 50 to 1500 to acquire TOF-MS/MS. The dynamic range enhancement was used throughout the MS experiment to ensure accurate mass measurements. The optimized MS analysis conditions were set as follows: nebulizer gas (GS 1), 55 psi; heater gas (GS 2), 55 psi; curtain gas (CUR), 40 psi; ion spray voltage floating (ISVF), 4500 V; turbo spray temperature (TEM), 550°C; declustering potential, -100 V; collision energy, -40 V.

Cai Z., Chen H., Chen J., Yang R., Zou L., Wang C., Chen J., Tan M., Mei Y., Wei L., Yin S, & Liu X. (2020). Metabolomics characterizes the metabolic changes of Lonicerae Japonicae Flos under different salt stresses. PLoS ONE, 15(12), e0243111.

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