Concentrations of HMGB1 in the culture supernatant of GB cells were determined using a Human ELISA Kit (#ARG81185, Arigo) according to the manufacturer’s instructions. IL-33, IL-1α, IL-37, IL-18, FGF1, FGF2, Galectin-3, MIF, Annexin A1, S100A8, IL-6 and CCL2 were measured by ELISA Kit (#EK0929, #EK0389, #EK1363, #EK0864, #EK0339, #EK0342, #EK0764, #EK0813, #EK1745, #EK1558, #EK0410, #EK0441, BOSTER). HMGB1 in GB patient sera was measured using an ELISA Kit (#6010, Chondrex). TNF-α, IL-8 and IFN-γ (#EL10019, #EL10008 and #EL10024, Anogen) and IL-1β (#DLB50, R&D) were measured by ELISA.
Dlb50
Manufactured by R&D Systems
Sourced in United States
The DLB50 is a compact and versatile laboratory centrifuge designed for a wide range of applications. It features a fixed-angle rotor with a maximum speed of 50,000 RPM and accommodates sample volumes up to 50 mL. The DLB50 is a reliable and efficient tool for researchers and laboratory professionals.
Automatically generated - may contain errors
Lab products found in correlation
D6050, by R&D Systems (13 mentions)
Dta00d, by R&D Systems (12 mentions)
Mlb00c, by R&D Systems (5 mentions)
Dla50, by R&D Systems (3 mentions)
Mta00b, by R&D Systems (3 mentions)
Db100b, by R&D Systems (2 mentions)
Dta00c, by R&D Systems (2 mentions)
Pi612, by Beyotime (1 mentions)
Ab219634, by Abcam (1 mentions)
Dif50c, by R&D Systems (1 mentions)
Mif00, by R&D Systems (1 mentions)
Difnb0, by R&D Systems (1 mentions)
Dcf300, by R&D Systems (1 mentions)
Mbs723281, by MyBioSource (1 mentions)
Catalyst dx chemistry analyzer, by IDEXX (1 mentions)
Ca 7000, by Sysmex (1 mentions)
Procyte dx hematology analyzer, by IDEXX (1 mentions)
Ab214091, by Abcam (1 mentions)
Ab196269, by Abcam (1 mentions)
G2000, by RayBiotech (1 mentions)
30 protocols using dlb50
1
Quantification of Cytokines in Glioblastoma
2
Quantifying Nasal Inflammatory Factors
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The concentrations of inflammatory factors IFN-γ, IL-1β, and IL-6 in nasal mucosal tissues were assessed using ELISA assays. A total of 1 mL 0.9% NaCl was added to each 0.1 g sample, and all samples were homogenized at 1000 rpm for 5 min and centrifuged at 1500 × g for 10 min at 4°C to collect the supernatant. Concentrations of inflammatory factors IFN-γ (DIF50C, R&D Systems, Minneapolis, MN, USA), IL-1β (DLB50, R&D Systems), IL-6 (D6050, R&D Systems), IL-4 (PI618, Beyotime), and IL-13 (ab47353, Abcam, Cambridge, MA, USA) in nasal mucosal tissues of patients with CRSsNP and controls were measured using ELISA kits.
The concentrations of inflammatory factors IFN-γ (MIF00, R&D Systems), IL-1β (MLB00C, R&D Systems), IL-6 (M6000B, R&D Systems), IL-4 (PI612, Beyotime), and IL-13 (ab219634, Abcam) in nasal mucosal tissues of mice in the CRSsNP and control groups were assessed using ELISA kits as well. All operations were performed strictly in accordance with the ELISA kit instructions.
The concentrations of inflammatory factors IFN-γ (MIF00, R&D Systems), IL-1β (MLB00C, R&D Systems), IL-6 (M6000B, R&D Systems), IL-4 (PI612, Beyotime), and IL-13 (ab219634, Abcam) in nasal mucosal tissues of mice in the CRSsNP and control groups were assessed using ELISA kits as well. All operations were performed strictly in accordance with the ELISA kit instructions.
3
Biomarker Quantification in Plasma
4
Cytokine Array and ELISA Analysis
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CM (1% FBS) from NCF or ILβ-treated NCF or IL1β-treated NCF plus neutralizing IL1β antibody was used for hybridization on the glass-slide human cytokine array G2000 from RayBiotech (Norcross, GA, USA), which detects 174 human cytokines. Samples were processed and analyzed by Tebu-Bio (Le Perray-en-Yvelines, France).
IL6 and IL1β were determined in supernatants obtained from cocultures CM by means of ELISA (R&D cat number D6050 and DLB50, respectively).
IL6 and IL1β were determined in supernatants obtained from cocultures CM by means of ELISA (R&D cat number D6050 and DLB50, respectively).
5
Evaluating Anti-Inflammatory Effects of Dapagliflozin in HK-2 Cells
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HK-2 cells were cultured in K-SFM serum-free medium in six-well plates. When the cells were 70–80% confluent, cells were divided into 3 groups: control (Cont), HG (30 mM), and HG (30 mM) with dapagliflozin (DAPA, 20 μM). After 48 h, supernatants of cultured medium were collected. Levels of pro-inflammatory cytokines in the cultured medium were detected by using human ELISA kits targeting IL-1β (DLB50, R&D, Minneapolis, MN, USA), IL-6 (D6050, R&D, Minneapolis, MN, USA), and TNFα (DTA00D, R&D, Minneapolis, MN, USA), according to the manufacturer’s instructions. The optical densities (O.D.) of samples were measured and corrected by subtracting the readings at 570 nm from the readings at 450 nm using an Emax Plus Microplate Reader (Molecular Devices, San Jose, CA, USA). The concentrations of pro-inflammatory cytokines in cultured medium were calculated by the standard curve and the O.D. of samples.
6
Quantification of Inflammatory Markers
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Quantification of IL-6, IL-1β, TNF-α and CCL2s in serum samples was performed by ELISA, employing kits from R&D Systems (D6050, DLB50, DTA00D and DCP00, respectively). Highly purified recombinant proteins were used to generate six-point standard curves (concentration range between 100 pg/ml and 3.13 pg/ml for IL-6; between 125 pg/ml and 3.9 pg/ml for IL-1β; between 1000 pg/ml and 15.6 pg/ml for TNF-α and between 1000 pg/ml and 31.3 pg/ml for CCL2). When specified in the manufacturer's protocol, serum samples were diluted 2-fold with the appropriate Calibrator Diluent. For each ELISA, 200 μl of sample or calibrator were loaded in each well of the provided 96-well plate. The optical density in each well was determined at 450 nm, using a SpectraMax Plus spectrophotometer (Molecular Devices), with wavelength correction set at 570 nm. A standard curve was generated for each protein using a four-parameter logistics (4-PL) curve-fit and, for each sample, the obtained concentrations of each cytokine/chemokine were multiplied by the appropriate dilution factor. Results are presented in pg/ml of serum.
7
Measuring Cytokine Levels in Cells and Plasma
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Supernatants from BMDMs were measured for mouse IL-1β (MLB00C, R&D systems), mouse IL-18 (7625, R&D systems), mouse TNF-α (MTA00B, R&D systems) according to the manufacturer’s instructions. Plasma in human subjects was measured for human IL-1β (DLB50, R&D systems) according to the manufacturer’s instructions.
8
Quantification of Protein Biomarkers
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Enzyme‐linked immunosorbent assay (ELISA) kits were used to detect the concentrations of human FGF19 (DF1900, R&D Systems), IL‐1α (DLA50, R&D Systems), IL‐1β (DLB50, R&D Systems) and complement C5a (ab193695, Abcam) in culture supernatants or serum as described.[36 ] The absorbance values could be read on a microplate reader (Molecular Devices) at a wavelength of 450 nm, within 30 min. The standard curve was used to convert absorbance values to protein concentrations.
9
Serum Biomarker Profiling in Preeclampsia
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Serum samples were collected from patients with sPE or a gestational age-matched normal pregnancy to detect the secreted IL-32 level using an enzyme-linked immunosorbent assay (ELISA) kit purchased from Sino Biological (IL-32 ELISA Kit, Human; Cat: KIT11064). The levels of IL-1β (DLB50, R&D Systems), TNFα (DTA00D, R&D Systems), sICAM-1 (ELH-ICAM1, RayBiotech, Peachtree Corners, GA, USA) and VCAM-1 (ELH-VCAM1, RayBiotech) in culture medium were measured with ELISA kits and conducted according to the standard procedure.
10
ELISA Kits for Cytokine Quantification
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Commercial ELISA kits (cat. nos. DTA00D, DLB50, D6050 and D1700; R&D Systems Inc.) were purchased to detect the serum levels of TNF-α, IL-1β, IL-6 and IL-17. All procedures, including sample and reagent preparation, assay procedure and calculation of the results were implemented according to the protocols of the kits.
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