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2 protocols using ki67 brilliant violet 711 ki 67

1

Flow Cytometric Analysis of Immune Cells

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The following antibodies (clone, company) were used for flow cytometric analysis at concentrations recommended by the manufacturer: CD4‐Alexa Fluor 488 (OKT4; eBioscience), Phospho Syk (Tyr348)‐PE (moch1ct; eBioscience), Galectin1‐Alexa Fluor 647 (GAL1/1831; Novus Biologicals), Phospho Lck (Tyr 394)‐PE (A18002D; BioLegend), Phospho Lck (Tyr505)‐PE (A17013A; BioLegend), Phospho BTK (Tyr223)‐PE (A16128B; BioLegend), Phospho ERK1/2 (Thr202, Tyr204)‐PerCP‐eFluor™ 710 (MILAN8R; Invitrogen), CD5‐PerCP/Cyanine5.5 (L17F12; BioLegend), CD43‐PE/Cy7 (CD4310G7; BioLegend), Ki67‐Brilliant Violet 711 (Ki‐67; BioLegend), CD19‐PE/Cy7 (HIB19; BioLegend), CD43‐PE (CD4310G7; BioLegend).
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2

Multiparametric Flow Cytometry Profiling

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The following antibodies (clone, company) were used for flow cytometric analysis at concentrations recommended by the manufacturer: CD4-Alexa Fluor 488 (OKT4, eBioscience), Phospho Syk (Tyr348)-PE (moch1ct, eBioscience), Galectin1-Alexa Fluor 647 (GAL1/1831, Novus Biologicals), Phospho Lck (Tyr 394)-PE (A18002D, BioLegend), Phospho Lck (Tyr505)-PE (A17013A, BioLegend), Phospho BTK (Tyr223)-PE (A16128B, BioLegend), Phospho ERK1/2 (Thr202, Tyr204)-PerCP-eFluor™ 710 (MILAN8R, Invitrogen), CD5-PerCP/Cyanine5.5 (L17F12, BioLegend), CD43-PE/Cy7 (CD4310G7, BioLegend), Ki67-Brilliant Violet 711 (Ki-67, BioLegend), CD19-PE/Cy7 (HIB19, BioLegend), CD43-PE (CD4310G7, BioLegend).
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