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Anti mouse secondary antibody conjugated to alexa 488

Manufactured by Thermo Fisher Scientific
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The Anti-mouse secondary antibody conjugated to Alexa-488 is a fluorescently-labeled antibody that binds to mouse primary antibodies. The Alexa-488 fluorophore attached to the secondary antibody emits green fluorescence when excited by light of the appropriate wavelength, allowing for the detection and visualization of target proteins or molecules in various bioanalytical applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (6 mentions) Gtx125928, by GeneTex (3 mentions) Anti rabbit secondary antibody conjugated to alexa 594, by Thermo Fisher Scientific (2 mentions) Mca400, by GeneTex (2 mentions) Mca400, by Bio-Rad (1 mentions) Zoe fluorescent cell imager, by Bio-Rad (1 mentions) Sp5 2 spectral confocalmicroscope, by Leica (1 mentions)

3 protocols using anti mouse secondary antibody conjugated to alexa 488

1

Influenza A Virus NP and M1 Immunostaining

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Influenza A/WSN/33 (H1N1) infected cells were fixed with 4% formaldehyde for 10 min followed by permeabilization with 0.2% Triton X-100 for another 10 min. After blocking with 10% bovine serum, cells were stained with mouse anti-NP (1:200 dilution; Bio-Rad: MCA400, Hercules, CA, USA) and rabbit anti-M1 (1:200 dilution; Gene Tex: GTX125928) antibodies, followed by incubation with anti-mouse secondary antibody conjugated to Alexa-488 and anti-rabbit secondary antibody conjugated to Alexa-594 (Thermo Fisher Scientific, Waltham, MA, USA). Nuclei were stained with 300 nM DAPI (Thermo Scientific) after secondary antibody incubation. Fluorescent images were acquired using a ZOE Fluorescent Cell Imager (Bio-Rad).
Hu Y., Zhang J., Musharrafieh R., Hau R., Ma C, & Wang J. (2017). Chemical Genomics Approach Leads to the Identification of Hesperadin, an Aurora B Kinase Inhibitor, as a Broad-Spectrum Influenza Antiviral. International Journal of Molecular Sciences, 18(9), 1929.
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2

Influenza A Viral Localization

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Influenza A/WSN/33 (H1N1)–infected cells were fixed with 4% formaldehyde for 10 min followed by permeabilization with 0.2% Triton X-100 for another 10 min. After blocking with 10% bovine serum, cells were sequentially stained with mouse anti-NP antibody (Bio-Rad: MCA400), rabbit anti-M1 antibody (Genetex: GTX125928), and anti-mouse secondary antibody conjugated to Alexa-488 (Thermo Scientific). Nuclei were stained with 300 nM DAPI (Thermo Scientific) after secondary antibody incubation. Fluorescent images were acquired using a Leica SP5-II spectral confocal microscope in Cancer center of the University of Arizona.
Hu Y., Zhang J., Musharrafieh R.G., Ma C., Hau R, & Wang J. (2017). Discovery of dapivirine, a Nonnucleoside HIV-1 Reverse Transcriptase Inhibitor, as a Broad-Spectrum Antiviral against Both Influenza A and B Viruses. Antiviral research, 145, 103-113.
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3

Influenza A Virus NP and M1 Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Influenza A/WSN/33 (H1N1) infected cells were fixed with 4% formaldehyde for 10 min followed by permeabilization with 0.2% Triton X-100 for another 10 min. After blocking with 10% bovine serum, cells were stained with mouse anti-NP antibody (Bio-Rad: MCA400) and rabbit anti-M1 (GeneTex: GTX125928) and followed by staining with anti-mouse secondary antibody conjugated to Alexa-488 and anti-rabbit secondary antibody conjugated to Alexa-594 (Thermo Scientific). Nucleus were stained with 300 nM DAPI (Thermo Scientific) after secondary antibodies incubation. Fluorescent images were acquired using a Leica SP5-II spectral Confocal Microscope (Leica).
Zhang J., Hu Y., Hau R., Musharrafieh R., Ma C., Zhou X., Chen Y, & Wang J. (2019). Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 133, 86-94.
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