Streptozotocin (STZ) (S0130) was procured from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) and Atripla, a fixed-dose combination antiretroviral drug (cART) was purchased from Bristol-Myers Squibb and Gilead Sciences (Foster City, CA, USA). The primary antibodies interleukin-1beta (IL-1β) (ab2105), interleukin-6 (IL-6) (ab9324), tumor necrosis factor-alpha (TNF-α) (ab6671), inducible nitric oxide synthase (iNOS) (ab115819), malondialdehyde (MDA) (ab243066), 8-hydroxydeoxyguanosine (8-OHDG) (ab62623), caspase 3 (ab4051), and Ki-67 (ab15580) were purchased from Abcam (Cambridge, MA, USA). The biotinylated goat anti-rabbit (BA-1000) and goat anti-mouse (BA-9200) secondary antibodies, and Avidin–Biotin Complex kit (ABC) (PK-6100) were purchased from Vector Laboratories (Burlingame, CA, USA).
Ab243066
Manufactured by Abcam
Sourced in United States, United Kingdom
Ab243066 is a laboratory reagent intended for research use. It is a monoclonal antibody that binds to a specific target protein. The core function of this product is to facilitate detection and analysis of the target protein in various experimental setups.
Automatically generated - may contain errors
Lab products found in correlation
Ab2105, by Abcam (1 mentions)
Ab115819, by Abcam (1 mentions)
Ab6671, by Abcam (1 mentions)
Ab4051, by Abcam (1 mentions)
Ab9324, by Abcam (1 mentions)
Gb21301, by Wuhan Servicebio Technology (1 mentions)
A11012, by Thermo Fisher Scientific (1 mentions)
Eclipse ti u, by Nikon (1 mentions)
A11001, by Thermo Fisher Scientific (1 mentions)
Tcs sp8 sted, by Leica (1 mentions)
A11005, by Thermo Fisher Scientific (1 mentions)
A11034, by Thermo Fisher Scientific (1 mentions)
Ab45977, by Abcam (1 mentions)
Bs 5810r, by Bioss Antibodies (1 mentions)
3 protocols using ab243066
1
Streptozotocin-Induced Diabetes Protocol
2
Immunohistochemical Analysis of MDA Expression
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The xenograft tumors of the mouse model were fixed with formalin and embedded with paraffin. 4-μm sections were obtained from the paraffin-embedded tissues. After warmed at 55 °C for 30 min, the sections were dewaxed and rehydrated in xylene and ethanol, respectively. Then, the sections were boiled in sodium citrate-hydrochloric acid buffer solution for 10 min and blocked with 1% goat serum for 20 min at room temperature. The sections were incubated with mouse monoclonal antibody to MDA antibody (ab243066, Abcam, Cambridge, UK) at a dilution of 1:100 overnight at 4 °C, followed by incubation with a CY3-labeled goat anti-mouse IgG secondary antibody (GB21301, Servicebio) for 30 min at 37 °C. The cell nucleus was stained with DAPI.
3
Immunofluorescence Analysis of Neuroinflammation
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At 1- and 3-days following ICH, the immunofluorescence was performed as previously prescribed [6 ]. Briefly, the frozen coronal slices were put into antigen repair solution and permeabilized with 1% Triton X-100 for 15min. Brain sections were blocked in 5% goat serum and incubated with anti-rabbit MPO (1:500, ab45977, Abcam), anti-rabbit NeuN (1:500, CST), anti-MDA [11E3] (1:200, ab243066, Abcam), anti-rabbit Iba1 (019–19741, Wako), anti-mouse Ly6G (551459, BD Biosciences), anti-mouse CD11b-FITC (Clone:M1/70, 1:200, 4306305, eBioscience), anti-rabbit Ltf (1:200, bs-5810R, Bioss) primary antibody at 4 °C overnight. Slices then incubated with goat anti-rabbit 594 (A11012, Invitrogen), goat anti-rabbit 488 (A11034, Invitrogen), goat anti-mouse 594 (A11005, Invitrogen), goat anti-mouse 488 (A11001, Invitrogen). Nuclei were visualized via a mounting medium containing DAPI (ZG1202, Vectarshield). Images were analyzed with a confocal microscope (TCS SP8 STED, Leica) and fluorescence microscope (ECLIPSE Ti–U, NIKON). Mean fluorescence intensity (MFI) was analyzed with ImageJ software.
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