Samples were kept at 4 °C throughout the sample preparation process. Apo PolθH was diluted to 0.9 mg/mL in SEC buffer, PolQHel-DNA complex was diluted to 1.25 mg/mL in low-salt SEC buffer, and samples were subject to centrifugation at 15,000 × g for 10 min. Quantifoil 300 mesh R 1.2/1.3 Holey Carbon Films were glow discharged under vacuum for 30 s at 15 mA in a Pelco easiGlow 91000 Glow Discharge Cleaning System (Ted Pella, Inc.). The sample (3 μL) was applied to the surface of the grid, blotted with Whatman 1 filter paper until 2 s after the liquid spot on the filter paper stopped spreading, and the grid was then immediately plunged into a liquid ethane pool cooled by liquid nitrogen using a manual plunge freezer in a 4 °C cold room with >95% humidity. Grids were then clipped under liquid nitrogen in preparation for imaging.
Pelco easiglow 91000 glow discharge cleaning system
Manufactured by Ted Pella
The Pelco easiGlow 91000 Glow Discharge Cleaning System is a laboratory equipment designed for cleaning and preparing surfaces prior to subsequent analysis or processing. It utilizes a glow discharge method to clean and activate surfaces, preparing them for various applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pelco easiglow 91000 glow discharge cleaning system
1
Cryo-EM Sample Preparation Protocol
2
Cryo-EM Imaging of Macromolecular Viral Assemblies
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!