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Ia6 2

Manufactured by BioLegend
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IA6-2 is a lab equipment product from BioLegend. It is a monoclonal antibody that binds to the integrin alpha 6 subunit.

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Lab products found in correlation

Pd 1 eh12.2h7, by BioLegend (3 mentions) Cd14 m5e2, by BioLegend (2 mentions) Cd4 okt4, by BioLegend (2 mentions) Hcd56, by BioLegend (2 mentions) Cd57 hnk 1, by BioLegend (2 mentions) Cd20 2h7, by BioLegend (2 mentions) Cd11c 3, by Thermo Fisher Scientific (2 mentions) Klrg1 sa231a2, by BioLegend (2 mentions) Cd86 it2, by BioLegend (2 mentions) Cd8b 2st8.5h7, by Beckman Coulter (2 mentions) Cd123 6h6, by BioLegend (2 mentions) Cd16 3g8, by BioLegend (2 mentions) Hla dr l243, by BioLegend (2 mentions) Cd19 hib19, by BioLegend (2 mentions) Attune nxt flow cytometer, by Thermo Fisher Scientific (2 mentions) Cd141, by BioLegend (1 mentions) Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (1 mentions) Cd27 m t271, by BioLegend (1 mentions) Sa231a2, by BioLegend (1 mentions) Ccr7 g043h7, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-human IgD-PerCP (IA6-2, IgD PerCP-Cy5.5 (IA6-2, IgD (clone IA6-2; IgD (IA6-2), PerCP/Cy5.5 anti-human IgD (IA6-2,

4 protocols using ia6 2

1

Multicolor Flow Cytometric Analysis of Immune Cell Subsets

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LIVE/DEAD™ Fixable Aqua Dead Cell Stain (L34957, Thermo Fisher) was used to exclude dead cells. CD1c (Biolegend), CD141 (Biolegend) were used for DCs analysis by flow cytometry. Fluorochrome-labeled anti-human mAbs specific for CD3 (UCHT1, Biolegend), CD4 (OKT4, Biolegend), CD45RA (HI100, Biolegend), CXCR5 (J252D4, Biolegend), CCR6 (G034E3, Biolegend), CXCR3 (G025H7, Biolegend), PD-1 (EH12.2H7, Biolegend) and ICOS (C398.4A, Biolegend) were used for analysis of Tfh cell population by flow cytometry. Fluorescein-labeled anti-human antibodies specific for CD3 (UCHT1, Biolegend), CD19 (HIB19, Biolegend), CD21 (Bu32, Biolegend), CD27 (O323, Biolegend), CD38 (HIT2, Biolegend) and IgD (IA6-2, Biolegend) were used for analysis of B cell population by flow cytometry.
Jiang Z., Lin H., Zhang H., Shi N., Zheng Z., Dong L., Yang Y, & Xia Q. (2022). An Integrative Analysis of the Immune Features of Inactivated SARS-CoV-2 Vaccine (CoronaVac). Vaccines, 10(6), 878.
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2

Flow Cytometric Profiling of PBMCs

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Frozen PBMCs were thawed, washed in FACS buffer (2% FBS, 1mM EDTA in PBS), and counted on TC20 (Biorad) before surface staining using two independent flow panels. For the innate panel, the following antibodies were used: CD3 (SP34, BD Pharmingen) and CD20 (2H7, BioLegend) for the exclusion of T & B lymphocytes, respectively. We further stained for CD56 (HCD56, Biolegend), CD57 (HNK-1, BioLegend), KLRG1 (SA231A2, BioLegend) CD16 (3G8, BioLegend), CD14 (M5E2, BioLegend), HLA-DR (L243, BioLegend), CD11c (3.9, ThermoFisher Scientific), CD123 (6H6, BioLegend) and CD86 (IT2.2, BioLegend). For the adaptive panel, the following antibodies were used: CD4 (OKT4, BioLegend), CD8b (2ST8.5H7, Beckman Coulter), CD45RA (HI100, TONBO Biosciences), CCR7 (G043H7, BD Biosciences), CD19 (HIB19, BioLegend), IgD (IA6–2, BioLegend), CD27 (M-T271, BioLegend), KLRG1 (SA231A2, BioLegend) and PD-1 (Eh12.2h7, BioLegend). Cells were stained with Ghost Dye viability dye (TONBO biosciences) for 30 minutes at 4C per manufacturer’s instructions, washed, surface stained with either innate or adaptive panels for 30 minutes at 4C. Samples were then washed and analyzed on Attune NxT Flow Cytometer (ThermoFisher Scientific, Waltham MA).
Sureshchandra S., Zulu M.Z., Doratt B., Jankeel A., Tifrea D., Edwards R., Rincon M., Marshall N.E, & Messaoudi I. (2021). Deep immune profiling of the maternal-fetal interface with mild SARS-CoV-2 infection. bioRxiv.
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3

Immunophenotyping of Frozen PBMCs

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Frozen PBMCs were thawed, washed in FACS buffer (2% FBS, 1 mM EDTA in PBS), and counted on TC20 (Biorad) before surface staining using two independent flow panels. For the innate panel, the following antibodies were used: CD3 (SP34, BD Pharmingen) and CD20 (2H7, BioLegend) for the exclusion of T & B lymphocytes, respectively. We further stained for CD56 (HCD56, Biolegend), CD57 (HNK-1, BioLegend), KLRG1 (SA231A2, BioLegend) CD16 (3G8, BioLegend), CD14 (M5E2, BioLegend), HLA-DR (L243, BioLegend), CD11c (3.9, ThermoFisher Scientific), CD123 (6H6, BioLegend) and CD86 (IT2.2, BioLegend). For the adaptive panel, the following antibodies were used: CD4 (OKT4, BioLegend), CD8b (2ST8.5H7, Beckman Coulter), CD45RA (HI100, TONBO Biosciences), CCR7 (G043H7, Biolegend), CD19 (HIB19, BioLegend), IgD (IA6-2, BioLegend), CD27 (M-T271, BioLegend), KLRG1 (SA231A2, BioLegend) and PD-1 (Eh12.2h7, BioLegend). Cells were stained with Ghost Dye viability dye (TONBO biosciences) for 30 min at 4C per manufacturer’s instructions, washed, surface stained with either innate or adaptive panels for 30 min at 4C. Samples were then washed and analyzed on Attune NxT Flow Cytometer (ThermoFisher Scientific, Waltham MA).
Sureshchandra S., Zulu M.Z., Doratt B.M., Jankeel A., Tifrea D., Edwards R., Rincon M., Marshall N.E, & Messaoudi I. (2022). Single-cell RNA sequencing reveals immunological rewiring at the maternal-fetal interface following asymptomatic/mild SARS-CoV-2 infection. Cell Reports, 39(11), 110938.
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4

Comprehensive B Cell Immunophenotyping

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PBMCs were first stained with fluorophore-labeled antibodies against cell surface markers CD3 (OKT3, BioLegend), CD19 (SJ25-C1, Thermo Fisher), CD20 (SI Appendix, Table S2), and CD27 (O323, BioLegend), fixed (Lysing Solution, BD Biosciences), permeabilized (Permeabilizing Solution 2, BD Biosciences), and stained with fluorophore-labeled antibodies against IgG (G18-145, BD Biosciences), IgA (8E10, Miltenyi Biotec), IgD (IA6-2, BioLegend), and IgM (MHM-88, BioLegend). The stained cells were acquired on a FACS Canto II flow cytometer (BD Biosciences) and analyzed in FlowJo software v9.9.6 (BD Biosciences).
Kardava L., Rachmaninoff N., Lau W.W., Buckner C.M., Trihemasava K., Blazkova J., Lopes de Assis F., Wang W., Zhang X., Wang Y., Chiang C.I., Narpala S., McCormack G.E., Liu C., Seamon C.A., Sneller M.C., O’Connell S., Li Y., McDermott A.B., Chun T.W., Fauci A.S., Tsang J.S, & Moir S. (2022). Early human B cell signatures of the primary antibody response to mRNA vaccination. Proceedings of the National Academy of Sciences of the United States of America, 119(28), e2204607119.
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