M 270 epoxy beads

Manufactured by Thermo Fisher Scientific

Sourced in United States

The M-270 Epoxy beads are non-porous, uniform polymer beads designed for use in a variety of affinity-based applications. The beads have an average diameter of 2.8 micrometers and are coated with an epoxy functional group, allowing for covalent coupling of ligands. The M-270 Epoxy beads provide a stable and versatile platform for the immobilization of proteins, peptides, oligonucleotides, and other molecules.

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Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Dynabeads M-270 Epoxy beads (17 citations) Dynabeads M-270 Epoxy magnetic beads (4 citations) M-270 Epoxy-coated magnetic beads (2 citations)

2 protocols using m 270 epoxy beads

Magnetic Bead-Antibody Conjugation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Batches of magnetic bead-antibody conjugates were made from Dynabeads® (M-270 Epoxy beads, Thermofisher) according to the manufacturer’s instructions at a ratio of 50:1 beads:antibody. The bead-antibody conjugate batches (“Ab-beads”) were quality control tested and stored in PBS at a concentration of beads at 10 mg/mL at 2–8 °C until use. Long term stability testing indicates a shelf life of at least 5 months at 2–8 °C (data not shown).

Bringans S., Ito J., Casey T., Thomas S., Peters K., Crossett B., Coleman O., Ebhardt H.A., Pennington S.R, & Lipscombe R. (2020). A robust multiplex immunoaffinity mass spectrometry assay (PromarkerD) for clinical prediction of diabetic kidney disease. Clinical Proteomics, 17, 37.

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Magnetic Bead-Antibody Conjugate Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Batches of magnetic bead-antibody conjugates were made from Dynabeads^® (M-270 Epoxy beads, Thermofisher, Waltham, MA, USA) according to the manufacturer’s instructions at a ratio of 50:1 beads:antibody. The bead-antibody conjugate batches (“Ab-beads”) were quality control tested and stored in PBS at a concentration of beads at 10 mg/mL at 2–8 °C until use. Long term stability testing indicates a shelf life of at least 5 months at 2–8 °C (data not shown).

Bringans S., Peters K., Casey T., Ito J, & Lipscombe R. (2020). The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease. Proteomes, 8(4), 31.

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