Cell extracts were separated on SDS polyacrylamide gel and blotted on nitrocellulose membranes (Millipore, St. Louis, MO, United States). Membranes were incubated with primer antibody overnight in 4°C, and hybridized with fluorescence secondary antibody for 1 h in room temperature. The list of antibodies used are as follows: anti-phospho-p38 MAPK (Thr180/Tyr182) (D3F9), anti-p38 MAPK (D13E1), anti-S6K, anti-phospho-S6K (Thr389), anti-S6, anti-phospho-S6 (Ser235/236), anti-4EBP1, anti-phospho-4EBP1 (Thr37/46) (Cell Signal Technology, Danvers, MA, United States), anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, United States), anti-Rps15 (EPR11104) (Abcam, Cambridge, United Kingdom), anti-β-actin (ab3280) (Abcam, Cambridge, United Kingdom), anti-HA (26D11) (Abmart, Shanghai, China), anti-Puromycin (12D10) (Sigma, St. Louis, MO, United States), anti-mouse 680 and anti-rabbit 800 secondary antibodies (LI-COR, Lincoln, NE, United States). ImageJ was used for optical density quantification of western blot analysis.
Anti β actin ab3280
Manufactured by Abcam
Sourced in United Kingdom
Anti-β-actin (ab3280) is a primary antibody that detects the beta-actin protein. Beta-actin is a commonly used housekeeping gene and its expression is often used as a control for normalizing sample loading in Western blot experiments.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose membrane, by Merck Group (1 mentions)
Anti phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti puromycin 12d10, by Merck Group (1 mentions)
Nf κb p65, by Cell Signaling Technology (1 mentions)
P tbk1 ser172, by Cell Signaling Technology (1 mentions)
Anti α tubulin ab52866, by Abcam (1 mentions)
Anti myc ta150121, by OriGene (1 mentions)
P nf κb p65, by Cell Signaling Technology (1 mentions)
P irf3 ser396, by Cell Signaling Technology (1 mentions)
2 protocols using anti β actin ab3280
1
Protein Expression Profiling by Western Blot
2
Signaling Pathway Antibody Protocol
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Antibodies specific for p-IKKε (Ser172) (#8766), IKKε (#3416), p-IRF3 (Ser396) (#4947), p-NF-κB p65 (#3033), NF-κB p65 (#8242), p-TBK1 (Ser172) (#5483), TBK1 (#3504), p-ERK (#4370), ERK (#4695), p-JNK (#4668), JNK (#9285), p-p38 (#9215), p38 (#8690), and MAVS (#4983) and HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-IRF3 (sc-15991) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-DDK (Flag) (TA50011-1), and anti-Myc (TA150121) were obtained from Origene (Rockville, MD, USA). Anti-TAOK1 (ab92601), anti-DYNC (ab23905), anti-α-tubulin (ab52866), and anti-β-actin (ab3280) were purchased from Abcam Biotechnology (Cambridge, UK). Anti-HA (51064-2-AP), anti-DDX58 (RIG-I) (20566-1-AP), and anti-MAP4 (11229-1-AP) were purchased from Proteintech (Wuhan, Hubei, China). Anti-p-MAP4 (Ser696) (#PA5-64526) was purchased from Invitrogen (Thermo Fisher Scientific, Scotts Valley, CA, USA).
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