Pe fitc mouse igg1 kappa isotype control

Ursolic acid (UA, purity > 90%) was purchased from Xi'an Ocean Biological Engineering Co., China. Compound US597 was synthesized as previously reported [22 (link)]. UA and US597 were dissolved in dimethyl sulfoxide (DMSO) and were used in all experiments. PE-labeled mouse anti-human ICAM-1, VCAM-1, integrin α5, α6 and integrin β1 mAbs, FITC-labeled mouse anti-human integrin α4 mAb, APC-labeled mouse anti-human E-selectin mAb, PE/FITC mouse IgG1 kappa isotype control and APC mouse IgG1 kappa isotype control antibody were all purchased from Becton Dickinson (BD) Pharmingen™. Human interleukin-1 beta (IL-1β) and mouse anti-human beta-actin (β-actin) antibody were purchased from Cell Signaling Technology. Fibronectin, matrigel and endothelial cell growth supplement (ECGS) were obtained from BD Biocoat™. The primary antibodies anti-ICAM-1, anti-integrin α6, anti-integrin β1 anti-E-selectin, anti-FAK, anti-Src, anti-paxillin and anti-PTEN were obtained from Abcam. The secondary antibody goat anti-mouse-IgG horseradish peroxidase was obtained from Promega. All other reagents used in this study were of the highest purity commercially available.

Ursolic acid (UA, purity > 90%) was purchased from Xi'an Ocean Biological Engineering Co. (Xian, China). Aspirin (Asp, purity > 90%) was purchased from Aladdin Reagent Company (Shanghai, China). UA, Asp and Asp-UA were dissolved in dimethyl sulfoxide (DMSO) and were used in all experiments. PE-labeled mouse anti-human E-cadherin, vimentin, EpCAM and integrin α1, α3, α5, α6 and β1 integrin mAbs, PE/FITC mouse IgG1 kappa isotype control and APC mouse IgG1 kappa isotype control antibody were all purchased from Becton Dickinson (BD) Pharmingen^TM (New Jersey, USA). Mouse anti-human beta-actin (β-actin) antibody was purchased from Cell Signaling Technology (Danfoss, USA). Matrigel were obtained from BD Biocoat^TM (New Jersey, USA). The primary anti-EGFR, anti-ERK, anti-MMP-2, anti-MMP-9, anti-COX-2, anti-E-cadherin, anti-β-catenin, anti-integrin α6, anti-integrin β1, anti-PTEN and anti-CD44 antibodies were obtained from Abcam Biotechnology (Cambridge, UK). The secondary antibody goat-anti-mouse-IgG or goat-anti-rabbit-IgG horseradish peroxidase was obtained from Promega (Madison, USA). All other reagents used in this study were of the highest purity commercially available.