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Caspase 1 p20 casper 1

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Caspase-1 p20 [Casper-1] is a recombinant protein that represents the p20 subunit of the human Caspase-1 enzyme. Caspase-1 is a cysteine protease involved in the inflammatory response and the processing of pro-inflammatory cytokines.

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Lab products found in correlation

Protease and phosphatase inhibitor, by Thermo Fisher Scientific (2 mentions) β actin antibody, by Cell Signaling Technology (2 mentions) C fos, by Cell Signaling Technology (2 mentions) 0.2 μm nitrocellulose membrane, by Bio-Rad (2 mentions) Nlrp3 d4d8t, by Cell Signaling Technology (2 mentions) Il 1β 3a6, by Cell Signaling Technology (2 mentions) Chemidoc mp imaging system, by Bio-Rad (2 mentions) Clarity western ecl substrate, by Bio-Rad (2 mentions) β actin 13e5, by Cell Signaling Technology (1 mentions) Anti il 1β 3a6, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Caspase-1 p20 Casper-1 Caspase-1

3 protocols using caspase 1 p20 casper 1

1

ATP-Induced Inflammasome Activation

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LPS-primed BMDM were stimulated with ATP for 90 min. Proteins in total cell lysates (prepared by RIPA buffer) and supernatants were detected by following mAbs: caspase-1 p20 (Casper-1, AdipoGen Life Science), anti-IL-1β (3A6, Cell signalling Technology), β-actin (13E5, Cell Signalling Technology).
Li X.Y., Moesta A.K., Xiao C., Nakamura K., Casey M., Zhang H., Madore J., Lepletier A., Aguilera A.R., Sundarrajan A., Jacoberger-Foissac C., Wong C., Cruz T.D., Welch M., Lerner A.G., Spatola B.N., Soros V.B., Corbin J., Anderson A.C., Effern M., Hölzel M., Robson S.C., Johnston R.L., Waddell N., Smith C., Bald T., Geetha N., Beers C., Teng M.W, & Smyth M.J. (2019). Targeting CD39 in cancer reveals an extracellular ATP and inflammasome driven tumor immunity. Cancer discovery, 9(12), 1754-1773.
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2

Protein Expression and Immunoblotting

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Whole cell lysates were suspended in RIPA buffer containing protease and phosphatase inhibitors (Thermo Scientific). Lysates were separated in SDS-PAGE and transferred to a nitrocellulose membrane, 0.2 μm (Bio-Rad Laboratories). The membrane was incubated with the following primary antibodies: phospho-MPK1/MPK2 polyclonal [Ser296, Ser318] (Thermo Scientific) and c-Fos (Cell Signaling), IL-1β [3A6] (Cell Signaling), Caspase-1 p20 [Casper-1] (Adipogen Life Sciences) (Thermo Scientific), NLRP3 [D4D8T] (Cell Signaling). Normalization was performed by probing the membrane with β-Actin antibody (Cell Signaling). Chemiluminescence detection was performed with Clarity™ Western ECL Substrate (Bio-Rad Laboratories), using the ChemiDoc™ MP Imaging System (Bio-Rad).
Drummond R.A., Swamydas M., Oikonomou V., Zhai B., Dambuza I.M., Schaefer B.C., Bohrer A.C., Mayer-Barber K.D., Lira S.A., Iwakura Y., Filler S.G., Brown G.D., Hube B., Naglik J.R., Hohl T.M, & Lionakis M.S. (2019). CARD9+ Microglia Promote Antifungal Immunity via IL-1β and CXCL1-mediated Neutrophil Recruitment. Nature immunology, 20(5), 559-570.
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3

Protein Expression and Immunoblotting

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Whole cell lysates were suspended in RIPA buffer containing protease and phosphatase inhibitors (Thermo Scientific). Lysates were separated in SDS-PAGE and transferred to a nitrocellulose membrane, 0.2 μm (Bio-Rad Laboratories). The membrane was incubated with the following primary antibodies: phospho-MPK1/MPK2 polyclonal [Ser296, Ser318] (Thermo Scientific) and c-Fos (Cell Signaling), IL-1β [3A6] (Cell Signaling), Caspase-1 p20 [Casper-1] (Adipogen Life Sciences) (Thermo Scientific), NLRP3 [D4D8T] (Cell Signaling). Normalization was performed by probing the membrane with β-Actin antibody (Cell Signaling). Chemiluminescence detection was performed with Clarity™ Western ECL Substrate (Bio-Rad Laboratories), using the ChemiDoc™ MP Imaging System (Bio-Rad).
Drummond R.A., Swamydas M., Oikonomou V., Zhai B., Dambuza I.M., Schaefer B.C., Bohrer A.C., Mayer-Barber K.D., Lira S.A., Iwakura Y., Filler S.G., Brown G.D., Hube B., Naglik J.R., Hohl T.M, & Lionakis M.S. (2019). CARD9+ Microglia Promote Antifungal Immunity via IL-1β and CXCL1-mediated Neutrophil Recruitment. Nature immunology, 20(5), 559-570.
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