14c camp

G418, Lipofectamine and Optimem were obtained from Invitrogen (Paisley, UK). FCS was obtained from PAA Laboratories (Wokingham, UK), L-glutamine from Lonza (Basel, Switzerland) and Fugene HD from Promega (Southampton, UK). Pertussis toxin (PTx) was purchased from Merck Chemicals (Nottingham, UK). NECA [5-(N-ethylcarboxamido)adenosine] and HEMADO [2-(1-hexynyl)-N-methyladenosine] were obtained from Tocris Bioscience (Bristol, UK). The AlphaScreen SureFire p-ERK assay kit was obtained from PerkinElmer (Waltham, MA, USA). [³H]-adenine and [¹⁴C]-cAMP were from Perkin-Elmer (Buckinghamshire, UK). CA200645 was purchased from Cellaura Technologies Ltd (Nottingham, UK). All other chemicals and reagents were purchased from Sigma-Aldrich (Gillingham, UK).

[³H]-adenine, [³H]-CGP 12177, [¹⁴C]-cAMP, Microscint-20 and Ultima Gold Scintillation fluid were from Perkin Elmer (Coventry, West Midlands, UK). CGP 12177, CGP 20712A, cimaterol and ICI 118551 were from Tocris Bioscience (Bristol, UK). Decyl-β-D-maltopyranoside was from Anatrace (Berkshire, UK). Purified β₁-m23-StaR [8] (link) was provided by Heptares Therapeutics. The following antibodies were used: goat-anti-mouse-Rhodamine secondary antibody (Molecular Probes, Life Technologies, Paisley, UK), horse radish peroxidase-conjugated secondary antibody (Cell Signalling Technology, Leiden, The Netherlands). BODIPY-TMR-CGP (CGP-12177-TMR) was purchased from Molecular Probes (Eugene, OR, USA). Fugene HD transfection reagent and furimazine were from Promega (Southampton, UK). Purified turkey and human ECL2 peptides were obtained from Cambridge Research Biochemicals (Cambridge, UK). All other reagents were from Sigma-Aldrich (Gillingham, UK).