The protein levels were measured after different time points of incubation by flow cytometry in a BD FACS Canto II Flow Cytometer and analysed with FlowJo software (FlowJo, LLC). Cells were rinsed twice with PBS (1X) and resuspended with PBS and Ghost Dye Red 780 (1:1000 dilution, Tonbo Biosciences) for 30 min on ice. After that, cells were rinsed once with PBS-0.2% Bovine Serum Albumin (BSA) (PanReac AppliChem) and resuspended in 50 μl of this buffer with the PE anti-human CD279 (PD-1) (EH12.2H7, 1:100 dilution, BioLegend) antibody for 30 min on ice. The analysis strategy was to compare the mean fluorescence intensity between treated and untreated live cells for oxLDL experiments. For anti-CD69 monoclonal antibody engagement experiments, samples were incubated with mouse serum (1:100 dilution, Sigma-Aldrich) for 15 min before adding the same volume with flow cytometry antibodies. Percentage of positive cells for PD-1 were represented at different times.
Pe anti human cd279 pd 1 eh12.2h7
Manufactured by BioLegend
PE anti-human CD279 (PD-1) (EH12.2H7) is a fluorescently-labeled antibody that binds to the human CD279 (PD-1) protein. CD279, also known as programmed cell death protein 1 (PD-1), is a cell surface receptor that plays a role in the regulation of the immune response.
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Lab products found in correlation
2 protocols using pe anti human cd279 pd 1 eh12.2h7
1
Flow Cytometry Analysis of PD-1 Expression
2
PD-1 Expression Quantification in Cells
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The protein levels were measured after different time points of incubation by ow cytometry in a FACS Canto cytometer and analyzed with FlowJo software (FlowJo, LLC). Cells were rinsed twice with PBS (1X) and resuspended with PBS and Ghost Dye Red 780 (1:1000 dilution, Tonbo Biosciences) for 30 min on ice. After that, cells were rinsed once with PBS-0.2% Bovine Serum Albumin (BSA) (PanReac AppliChem) and resuspended in 50 µl of this buffer with the PE anti-human CD279 (PD1) (EH12.2H7, 1:100 dilution, BioLegend) antibody for 30 min on ice. The analysis strategy was to compare the mean uorescence intensity between treated and untreated live cells for oxLDL experiments. For anti-CD69 monoclonal antibody engagement experiments, samples were incubated with mouse serum (1:100 dilution, Sigma-Aldrich) for 15 min before adding the same volume with ow cytometry antibodies. Percentage of positive cells for PD-1 were represented at different times.
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