Gr159897

Manufactured by Bio-Techne

Sourced in United Kingdom

GR159897 is a lab equipment product offered by Bio-Techne. It is a compact and versatile device designed for precise and efficient sample preparation and processing in various laboratory applications. The core function of GR159897 is to provide a reliable and consistent means of handling and processing samples, ensuring accurate and reproducible results.

Automatically generated - may contain errors

Lab products found in correlation

Guanethidine monosulphate, by Merck Group (3 mentions) Sb218795, by Bio-Techne (3 mentions) L name, by Bio-Techne (2 mentions) Atropine sulphate, by Merck Group (2 mentions) L 732 138, by Bio-Techne (2 mentions) Biotinylated anti mouse igg antibody, by Vector Laboratories (1 mentions) Atropine sulfate, by Merck Group (1 mentions) Substance p, by Bio-Techne (1 mentions) 6 hydroxydopamine, by Merck Group (1 mentions) Nω nitro l arginine methyl ester, by Merck Group (1 mentions) Tetrodotoxin, by Bio-Techne (1 mentions) Mouse anti th antibody, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Cp99994, by Bio-Techne (1 mentions) Carbachol cch, by Merck Group (1 mentions) Mrs 2500 tetraammonium salt, by Bio-Techne (1 mentions) Cp 96345, by Bio-Techne (1 mentions) Poly 1 c hmw, by InvivoGen (1 mentions) Anti mouse cd11c n418, by BioLegend (1 mentions) 7 aminoactinomycin d 7 aad, by Beckman Coulter (1 mentions)

12 protocols using gr159897

Neurochemical Profiling of Dopamine Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Atropine sulfate, guanethidine monosulphate, N^ω-nitro-L-argininemethylester, 6-hydroxy dopamine, and ascorbic acid were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Tetrodotoxin, Substance P, N-acetyl-L-tryptophan 3,5-bis (trifluoromethyl) benzyl ester, GR159897, and SB218795 were purchased from Tocris (Bristol, UK). Mouse anti-TH antibody (1:2000) was purchased from Chemicon International (Temecula, CA, USA). Biotinylated anti-mouse IgG antibody (1:1000) and nickel-intensified 3,3′-diaminobenzidine tetrahydrochloride (DAB Substrate Kit for peroxidase) were purchased from Vector Laboratories (Burlingame, CA, USA). Neutral-buffered formaldehyde (NBF) and xylene were purchased from Carlo Erba (Milan, Italy).

Pellegrini C., Fornai M., Colucci R., Tirotta E., Blandini F., Levandis G., Cerri S., Segnani C., Ippolito C., Bernardini N., Cseri K., Blandizzi C., Haskó G, & Antonioli L. (2016). Alteration of colonic excitatory tachykininergic motility and enteric inflammation following dopaminergic nigrostriatal neurodegeneration. Journal of Neuroinflammation, 13, 146.

+ Open protocol

+ Expand

DNBS-induced colitis in mice

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acute colitis was induced in male and female mice under isoflurane anesthesia by an enema of 0.1 mL of a solution containing 5 mg dinitrobenzene sulfonic acid (DNBS) dissolved in 50% ethanol.²⁶ (link) Control animals were given enemas of saline. Subsets of mice received daily injections (intraperitoneally) of either 0.5 mg/kg GR 159897 (Tocris, Minneapolis, MN) in 0.1% dimethyl sulfoxide or 0.1% dimethyl sulfoxide over 1, 3, 7, or 21 days beginning 24 hours before the induction of colitis. Mice were monitored closely and weight loss was recorded daily for the first week and once every other day for the following 2 weeks. Mice were killed at 24 hours, 48 hours, 7 days, or 3 weeks after induction of colitis. Macroscopic damage was recorded with an established scoring system.²⁷ (link)

Delvalle N.M., Dharshika C., Morales-Soto W., Fried D.E., Gaudette L, & Gulbransen B.D. (2018). Communication Between Enteric Neurons, Glia, and Nociceptors Underlies the Effects of Tachykinins on Neuroinflammation. Cellular and Molecular Gastroenterology and Hepatology, 6(3), 321-344.

+ Open protocol

+ Expand

Chemical Reagents and Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Atropine was purchased from Wako Pure Chemical (Osaka, Japan). Carbachol (CCh) and pyr3
were purchased from Sigma-Aldrich (St. Louis, MO, USA). CP99994 and GR159897 were
purchased from Tocris Bioscience (Bristol, UK). Neurokinin (NK) A and substance P (SP)
were purchased from Peptide Institute (Osaka, Japan).

AZUMA Y.T., SUZUKI S., NISHIYAMA K, & YAMAGUCHI T. (2021). Gastrointestinal motility modulation by stress is associated with reduced smooth muscle contraction through specific transient receptor potential channel. The Journal of Veterinary Medical Science, 83(4), 622-629.

+ Open protocol

+ Expand

Investigating Neurotransmitter Receptor Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless otherwise stated, all chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO). GR 159897 (5-fluoro-3-[2-(4-methoxy-4-[([R]-phenylsulphinyl)methyl]-1-piperidinyl)ethyl]-1H-indole), MRS 2500 tetraammonium salt ([1R*,2S*]-4-[2-lodo-6-(methylamino)-9H-purin-9-yl]-2-[phosphonooxyl]bicyclo[3.1.0]hexane-1-methanol dihydrogen phosphate ester tetraammonium salt), and CP 96345 ([2S,3S]-N-[2-methoxyphenyl]methyl-2-diphenylmethyl-1-azabicyclo[2.2.2]octan-2-amine) were purchased from Tocris. NKA was purchased from Abcam (Cambridge, MA). The Cx43 mimetic peptide, 43Gap 26, was purchased from Anaspec (Fremont, CA). Di-nitro benzenesulfonic acid (2,4-dinitrobenzenesulfonic acid dihydrate) was purchased from MP Biomedicals (Solon, OH).

+ Open protocol

+ Expand

Characterizing NK Cell Responses Using Antibodies and Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescent dye‐conjugated anti‐CD45 (30‐F11) and anti‐mouse CD11c (N418) were obtained from BioLegend or BD Biosciences. 7‐amino‐actinomycin D (7AAD) was purchased from Beckman Coulter. Rabbit polyclonal antibody against NK2R (M‐48) was purchased from Santa Cruz Biotechnology, Inc. Recombinant murine IFN‐α15 (752802) and human IFN‐α2 (592702) were purchased from BioLegend and murine IFN‐β (8234‐MB) was from R&D systems Inc. Recombinant human IFN‐β (092–06061) and neurokinin A agonist (140–07171) were purchased from Wako Pure Chemical Industries. An NK2R‐selective antagonist (GR 159897) was purchased from TOCRIS Bioscience. Poly I:C (HMW) was purchased from InvivoGen. A selective JAK1/2 inhibitor (ruxolitinib) was purchased from abcam.

Xiang H., Toyoshima Y., Shen W., Wang X., Okada N., Kii S., Sugiyama K., Nagato T., Kobayashi H., Ikeo K., Hashimoto S., Tanino M., Taketomi A, & Kitamura H. (2022). IFN‐α/β‐mediated NK2R expression is related to the malignancy of colon cancer cells. Cancer Science, 113(8), 2513-2525.

+ Open protocol

+ Expand

Biochemical Assays for Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aβ was purchased by Biochem (Germany). Atropine sulphate, SP, guanethidine monosulphate, nigericin, Ac-YVAD-cmk, bacterial LPS (Escherichia coli 026:B6) and DMSO, sucrose, Tris, EGTA, Triton X-100, Tris-buffer, DTNB, acetil-coenzyme A, oxaloacetate and anti-β-actin-HRP (A3854) were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Foetal bovine serum (FBS) was obtained from PAA Laboratories. TTX, GR159897, SB218795, L-NAME were obtained from Tocris (Bristol, UK). Anti-IL-1β (anti-human) was purchased by R&D, rabbit anti-ASC (SC-22514-R) by Santa Cruz. Secondary antibody HRP conjugates were from DAKO. Alexa Fluor 594-conjugated donkey anti-rabbit antibody (A-21207) was purchased by Invitrogen.

Pellegrini C., Daniele S., Antonioli L., Benvenuti L., D’Antongiovanni V., Piccarducci R., Pietrobono D., Citi V., Piragine E., Flori L., Ippolito C., Segnani C., Palazon-Riquelme P., Lopez-Castejon G., Martelli A., Colucci R., Bernardini N., Trincavelli M.L., Calderone V., Martini C., Blandizzi C, & Fornai M. (2020). Prodromal Intestinal Events in Alzheimer’s Disease (AD): Colonic Dysmotility and Inflammation Are Associated with Enteric AD-Related Protein Deposition. International Journal of Molecular Sciences, 21(10), 3523.

+ Open protocol

+ Expand

Recombinant NK Receptor Expression in CHO Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human recombinant NK2 and NK1 receptors were stably expressed in CHO cells using the standard FuGENE protocol and vectors from Genecopeia (Rockville, USA). Stock solutions (10 mM) of GR159897 (Tocris, Bristol, UK), aprepitant (Selleck Chemicals, Houston, USA), GR64349 (Tocris, Minneapolis, USA), NKA and substance P (Sigma-Aldrich, St Luis, USA) were prepared in DMSO and frozen at −20°C until use. Further dilutions were made in DMSO to span a final concentration range of 0.01 nM to 10 μM.

Perdona E., Cavallini P., Sava A., Griffante C., Ricca D.J., Thor K.B., Rupniak N.M, & Corsi M. (2019). Potency, Efficacy, and Selectivity of GR64349 at Human Recombinant Neurokinin NK2 and NK1 Receptors. Neuroscience letters, 711, 134456.

+ Open protocol

+ Expand

Neuroinflammation Modulation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aβ, atropine sulphate, carbachol, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), guanethidine monosulphate, lipopolysaccharide (LPS), PEA and PBS were purchased from Sigma Aldrich (St. Louis, MO, United States). L-NAME, L-732,138, GR159897, SB218795, SP and TTX were purchased from Tocris (Bristol, United Kingdom).

D’Antongiovanni V., Pellegrini C., Antonioli L., Benvenuti L., Di Salvo C., Flori L., Piccarducci R., Daniele S., Martelli A., Calderone V., Martini C, & Fornai M. (2021). Palmitoylethanolamide Counteracts Enteric Inflammation and Bowel Motor Dysfunctions in a Mouse Model of Alzheimer’s Disease. Frontiers in Pharmacology, 12, 748021.

+ Open protocol

+ Expand

Neuromodulatory Agents in Cell Cultures

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

5-HT hydrochloride powder (Sigma) and octopamine hydrochloride (OA; Sigma) were dissolved in 0.1 M HCl. Both 5-HT and OA were freshly added to plates, and used at a final concentration of 5 mM (ref. 1 (link)). GR159897 (Tocris) was used at the final concentrations of 0.04, 0.016 and 0.008 mM in 10% dimethyl sulfoxide. The doses were chosen after ensuring that there were no developmental delays, or other overt effects on growth.

Palamiuc L., Noble T., Witham E., Ratanpal H., Vaughan M, & Srinivasan S. (2017). A tachykinin-like neuroendocrine signalling axis couples central serotonin action and nutrient sensing with peripheral lipid metabolism. Nature Communications, 8, 14237.

+ Open protocol

+ Expand

Synthesis and Characterization of LMN-NKA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gelatin type B and glycine (ACS agent, 98.5%) were purchased from Fisher Scientific (Pittsburg, PA, USA). Sorbitol Solution USP (70%) was obtained from Spectrum Chemical MFG Corp (Gardena, CA, USA). HPLC grade trifluoroacetic acid and acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO, USA). LMN-NKA was synthesized for Dignify Therapeutics (Durham, NC, USA) by Bachem America Inc (Torrance, CA, USA). GR159897 (NK₂ receptor antagonist; Tocris, Bristol, United Kingdom) was solubilized at 50 mM in DMSO and diluted with saline to a concentration of 1 mg/mL.

Bae J., Johnston T.A., Chaiittianan R., Sutthanut K., Jay M, & Marson L. (2017). Characterization and in vivo efficacy of a heptapeptide ODT formulation for the treatment of neurogenic bladder dysfunction. International journal of pharmaceutics, 536(1), 397-404.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!