Lsm 710 axioobserver inverted 34 channel confocal

Manufactured by Zeiss

The LSM 710 AxioObserver Inverted 34-Channel Confocal is a high-performance microscope system designed for advanced imaging applications. It features a 34-channel detection system, allowing for simultaneous acquisition of multiple fluorescent signals. The system is optimized for live-cell imaging and provides exceptional spatial and temporal resolution. Its inverted design enables convenient sample handling and observation.

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Lab products found in correlation

Cryostat, by Leica (4 mentions) Tissue tek oct compound, by Sakura Finetek (3 mentions) Nanozoomer, by Hamamatsu Photonics (2 mentions) Bz x700, by Keyence (1 mentions) A11008, by Thermo Fisher Scientific (1 mentions) A11122, by Thermo Fisher Scientific (1 mentions) Goat serum, by Merck Group (1 mentions) Nanozoomer 2.0 rs, by Hamamatsu Photonics (1 mentions)

Spelling variants of this product

LSM 710 (5147 citations) LSM 710 confocal (102 citations) LSM 710 AxioObserver (33 citations) LSM 710 inverted (10 citations) Confocal 710 (8 citations)

4 protocols using lsm 710 axioobserver inverted 34 channel confocal

Immunofluorescence Staining of Mouse Brain

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Mice were deeply anesthetized and transcardially perfused using PBS followed by 4% paraformaldehyde in PBS. Brains were post-fixed in fixative and stored in 30% sucrose in PBS overnight for cryoprotection. Brains were embedded and mounted with Tissue-Tek OCT compound (Sakura finetek) and 20 μm sections were cut using a cryostat (Leica). Brain slices were washed using PBS, permeabilized using PBST (0.3% Triton X-100 in PBS) for 30 min and then incubated with blocking solution (5% normal goat serum or normal donkey serum in PBST) for 1 hr followed by primary antibody incubation overnight at 4 °C using following antibodies:
The next day, slices were washed with PBS and incubated with appropriate secondary antibodies for 2 hrs (1:500, All from Invitrogen):
The slices were washed with PBS followed by counterstaining with DAPI or Hoechst and coverslipped. Fluorescence images were taken using a confocal microscope (LSM 710 AxioObserver Inverted 34-Channel Confocal, Zeiss) or Nanozoomer (Hamamatsu).

Chung S., Weber F., Zhong P., Tan C.L., Nguyen T., Beier K.T., Hörmann N., Chang W.C., Zhang Z., Do J.P., Yao S., Krashes M.J., Tasic B., Cetin A., Zeng H., Knight Z.A., Luo L, & Dan Y. (2017). Identification of Preoptic Sleep Neurons Using Retrograde Labeling and Gene Profiling. Nature, 545(7655), 477-481.

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Immunofluorescence Staining of Mouse Brain

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Detecting GAD2 mRNA Expression by FISH

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FISH was performed to detect GAD2 mRNA. Brains were embedded and mounted with Tissue-Tek OCT compound (Sakura finetek), and 30 μm sections were cut using a cryostat (Leica). FISH was performed using an RNAscope assay according to the manufacturer’s instructions (RNAscope® Fluorescent Multiplex Reagent Kit, Cat. # 320850, Advanced cell Diagnostics). Sections were hybridized with a GAD2 probe (Mm-Gad2, 439371) and eGFP probe (EGFP-C3, 400281-C3), and amplification steps were carried out followed by Hoechst staining. Fluorescence images were taken using a confocal microscope (LSM 710 AxioObserver Inverted 34-Channel Confocal, Zeiss) or a fluorescence microscope (BZ-X700, Keyence).

Weber F., Hoang Do J.P., Chung S., Beier K.T., Bikov M., Saffari Doost M, & Dan Y. (2018). Regulation of REM and Non-REM Sleep by Periaqueductal GABAergic Neurons. Nature Communications, 9, 354.

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Immunohistochemical Analysis of ChR2-Expressing Neurons

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Mice were deeply anesthetized and transcardially perfused with 0.1 M phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in PBS. After removal, brains stayed overnight in 4% paraformaldehyde. For cryoprotection, brains were stored in 30% sucrose (w/v) in PBS solution for at least 1 night. Brains were sliced in 30 or 40 μm sections using a cryostat (Leica). For immunohistochemistry, non-specific binding sites were blocked by incubating the brain sections in 2% goat serum (Millipore) in PBST (0.3% Triton X-100 in PBS).
To amplify the fluorescence of axon fibers expressing ChR2-eYFP or eYFP, we applied antibodies for GFP (A11122, Life Technologies, 1:1000). Brain sections were incubated with the primary antibody diluted in blocking solution for 2 nights. A species-specific secondary antibody conjugated with green Alexa fluorophore (A11008, Life Technologies, 1:1000; goat anti-rabbit) was diluted in PBS and applied for 2 h. Fluorescence images were taken using a confocal microscope (LSM 710 AxioObserver Inverted 34-Channel Confocal, Zeiss), fluorescence microscope (Keyence, BZ-X710), and Nanozoomer 2.0 RS (Hamamatsu).

Weber F., Hoang Do J.P., Chung S., Beier K.T., Bikov M., Saffari Doost M, & Dan Y. (2018). Regulation of REM and Non-REM Sleep by Periaqueductal GABAergic Neurons. Nature Communications, 9, 354.

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