Confocal software 2
Leica Confocal Software 2.5 is a software package designed to operate Leica confocal microscopes. It provides the core functionality required to control the microscope hardware, acquire and process confocal images.
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5 protocols using confocal software 2
Fluorescence Microscopy of NTHi Cells
Confocal microscopy of bacterial biofilms
Samples were washed three times to remove nonspecific staining, and fluorescence was observed by confocal laser microscopy. Images of the double-labeled sections were acquired with a Leica TCS-SL filter-free spectral confocal laser scanning microscope (Leica Microsystems, Inc.) with a 488-nm argon laser, 543- and 633-nm He/Ne lasers (Centres Científics i Tecnològics, Campus de Bellvitge, Universitat de Barcelona, Barcelona, Spain), a 63× oil immersion objective (1.4 numerical aperture), and an image resolution of 1,024 by 1,024 pixels. The images were acquired randomly from the cover glass surface and analyzed with the Leica Confocal Software 2.5 (Leica Microsystems, Inc.).
Microscopic Imaging of GUS, GFP, and Esculin in Plants
Microscopic Analysis of GUS Plants
Images of protoplasts and GFP-reporter plants were taken on a confocal laser scanning microscope (Leica TCS SPII; Leica Microsystems) using a 488nm argon laser for excitation and processed with Leica Confocal Software 2.5. Detection windows ranged from 497 to 526nm for GFP and from 682 to 730nm for chlorophyll autofluorescence.
Confocal Microscopy of Fluorescent Biological Samples
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