Cells (5 × 105) were seeded in 6-well plates after transfection, and then,0.3 μg/ml DOX was added to the medium. After incubation for 48 h, the adherent cells were collected and washed twice with cold PBS. Then, the cells were fixed in ice cold 70% ethanol. The cell cycle distribution was analyzed using the Cell Cycle Analysis Kit (MultiSciences, China). The percentage of cells in different phases of each cell cycle was sorted using a ModFit 5.2 computer program.
Cell cycle analysis kit
Manufactured by MultiSciences Biotech
Sourced in China
The Cell Cycle Analysis Kit is a laboratory tool designed to study the phases of the cell cycle. It provides the necessary reagents and protocols to analyze the distribution of cells in different stages of the cell cycle, such as G1, S, and G2/M phases.
Automatically generated - may contain errors
Lab products found in correlation
Facscan flow cytometer, by BD (3 mentions)
Annexin 5 pe 7 aad apoptosis detection kit 1, by BD (2 mentions)
5 ethynyl 2 deoxyuridine edu kit, by RiboBio (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Flow cytometry, by BD (1 mentions)
Accuri c6 plus flow cytometer, by BD (1 mentions)
Flow cytometry, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc pi apoptosis kit, by MultiSciences Biotech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Dreamtaq green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Qiaamp dna mini kit, by Qiagen (1 mentions)
Epitect bisulfite kit, by Qiagen (1 mentions)
Cell counting kit 8 cck 8, by MultiSciences Biotech (1 mentions)
Annexin 5 pi apoptosis detection kit 1, by BD (1 mentions)
Facscalibur system, by BD (1 mentions)
Facs caliber system, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
21 protocols using cell cycle analysis kit
1
Cell Cycle Analysis of Dox-Treated Cells
2
Evaluating TNBC Cell Growth
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According to the manufacturer's instructions, we applied the cell counting kit-8 (CCK8, Beyotime, Shanghai, China), clone formation, and 5-ethynyl-2′-deoxyuridine (EdU) kit (RiboBio, Guangzhou, China) to demonstrate the growth ability of TNBC.
For cell cycle analysis, chosen cells were collected and washed, then fixed in 70% ethanol at −20°C overnight before FCM. Cell cycle analysis kit (MultiSciences, Hangzhou, China) was used to dye for 15 min. Data were analyzed by a FACScan flow cytometer.
For cell cycle analysis, chosen cells were collected and washed, then fixed in 70% ethanol at −20°C overnight before FCM. Cell cycle analysis kit (MultiSciences, Hangzhou, China) was used to dye for 15 min. Data were analyzed by a FACScan flow cytometer.
3
Cell Cycle Analysis in Transfected Cells
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Transfected cells were harvested and washed with cold PBS twice follow by fixed in cold 70% ethanol. The Cell Cycle Analysis Kit (MultiSciences, China) was used to analyze the cell cycle distribution. The software ModFit 5.2 was used to sort and calculate the cells in different phases of cell cycle.
4
Cell Cycle Analysis by Flow Cytometry
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Cells were collected, washed and fixed under the condition of −20 °C for one day. The cells were washed again, stained using propidium iodide, and treated with a cell cycle analysis kit (MultiSciences, Hangzhou, China) according to the manufacturer's instructions. The cell cycle characteristics were then analyzed using a FACScan flow cytometer (BD Bioscience, Franklin Lakes, NJ, USA). The gray is the G1 phase, the purple is the S phase, the blue is the G2 phase.
5
Cell Cycle Analysis via Flow Cytometry
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Cells (5 × 105 cells) were collected in 75% alcohol at −20 °C overnight. The cell suspension was then mixed with propidium iodide (PI) solution in the Cell Cycle Analysis Kit (Multisciences) and incubated at room temperature for 30 min. Flow cytometry was used to determine the proportion of cells in different cell-cycle phases. The results were analyzed using the FlowJo V10 software.
6
Cell Cycle Analysis of Erastin-Treated Cells
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The control group cells and experimental group cells treated with 5 µM erastin treatment for 24 h were prepared in a 6-well plate. Approximately 1 × 106 cells were washed with PBS and centrifuged to obtain the cells. After the cells were incubated with the mixture in the Cell Cycle Analysis Kit (MultiSciences, China), the flow cytometry (BD company, USA) and ModFit LT software were used to analyze the cell cycle distribution.
7
Cell Cycle Analysis of SCARNA12 Knockdown
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T24-WT, T24 SCARNA12-KD, UMUC3-WT, and UMUC3 SCARNA12-KD cells (2 × 105) were plated in 6-well plates and incubated for 48 h. Subsequently, cells were harvested, fixed in 70% ethanol at −20 °C for 24 h, and subjected to cell cycle analysis using the Cell Cycle Analysis Kit (MultiSciences, Hangzhou, China). Flow cytometry was performed on an Accuri C6 Plus flow cytometer (BD Biosciences, San Jose, CA, USA), and ModFit software 5.2 (Verity Software House, Topsham, ME, USA) was employed to calculate the proportion of cells in different phases of this analysis.
8
Cell Cycle Quantification Protocol
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The cell cycle was quantified using the manufacturer’s instructions for the cell cycle analysis kit (Multi Science, Hangzhou, China). A single-cell suspension of H460 and H157 cells was prepared by trypsinization and washed once with phosphate-buffered saline (PBS). Subsequently, the cells were centrifuged (1000× g rpm, 5 min) and resuspended in 300 μL PI containing 3 μL immobilization in a dark place for 30 min. The samples were immediately analyzed by flow cytometry (Thermo Fisher Scientific, Waltham, MA, USA).
9
Cell cycle and apoptosis analysis
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Cells were seeded at a density of 5 × 105 cells per well in 6-well plates. After treatment, cells were fixed in ice-cold 70 % ethanol. Cell cycle distribution was analyzed using the Cell Cycle Analysis Kit (MultiSciences, China) and apoptosis was detected using the Annexin V-PE/7-AAD Apoptosis Detection Kit I (BD BioSciences, USA) according to the manufacturer’s instruction. Stained cells were examined by FACSCalibur flow cytometry.
10
Cell Cycle Analysis by Flow Cytometry
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Cells were collected and washed carefully with PBS and fixed with 75% ethanol at −20 °C. After 24 h fixation, the cells were washed with PBS again and stained with propidium iodide using the cell cycle analysis kit (MultiSciences, Hangzhou, China) for 15 min. Cell cycle features were analysed using a FACScan flow cytometer (BD Bioscience, Franklin Lakes, NJ, USA).
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