The mouse neuroblastoma cell line Neuro-2a (N2a) was purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). The N2a cells were cultured in Dulbecco’s modified Eagle’s medium nutrient mixture F-12 (DMEM-F12, Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Irvine, CA, USA) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin), and then incubated in a humidified atmosphere with 5% CO2 at 37°C. The high-salt N2a cell model was established by treating them with NaCl medium (containing an additional 40 mM NaCl) for 24 h [16 (link)] (NaCl group). Non-treated N2a cells were placed into a control (CON) group.
Neuro 2a
Manufactured by Procell
Sourced in China
The Neuro-2a is a cell line derived from a mouse neuroblastoma. It is a commonly used in vitro model for studying neurological processes and functions.
Automatically generated - may contain errors
4 protocols using neuro 2a
1
Establishing a High-Salt Mouse Neuroblastoma Model
2
Differentiation and Lipid Metabolism in Neuro-2a Cells
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Neuro-2a (N2a; mouse neuroblastoma cells) was provided by Procell Life Science&Technology Co., Ltd. N2a cells were cultured in Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F12, Gibco, Carlsbad, CA, USA) and supplemented with 10% fetal bovine serum (FBS, Invigentech, Irvine, CA, USA) and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin). The culture was incubated in a humidified atmosphere with 5% CO2 at 37°C. After 24 h of differentiation, the cells were disposed of normal medium, palmitic acid (200 μM for 24 h) [21 (link)] (Kunchuang Biotechnology, Xi'an, China) and palmitic acid + Kaem (kaemferol, 50 μM for 24 h) (HY-14590, MCE).
3
Cell Culture Protocols for Neuroscience Research
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The PC12, BV2, SH-SY5Y, and Neuro-2a cell lines were bought from Procell Life Science & Technology Co., Ltd. The bEnd.3 cell line was provided by the American Type Culture Collection (ATCC). The HT-22 cell line was obtained from Sunncell Biotech Inc. The PC12 cell line and Neuro-2a cell line were respectively cultured in the RPMI 1640 medium and MEM medium. The BV2, SH-SY5Y, HT-22, and bEnd.3 cell lines were grown in the DMEM medium.
4
Investigating HS Diet Impact on Neuro-2a Cells
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To investigate the impact of an HS diet on neuronal cells, we established a high-salt cell model using the Neuro-2a (N2a) cell line, which was provided by Procell Life Science & Technology Co., Ltd. (Wuhan, China) The N2a cells were maintained in a humidified incubator at 37 °C and 5% CO2 and were cultured in Dulbecco's modified Eagle's medium nutrient mixture F-12 (DMEM-F12, Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Invitrogen, Irvine, CA, USA) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin)18 (link). N2a cells were treated with the medium supplemented with NaCl (adding 80 mM NaCl) for 48 h to establish the HS N2a cell model23 (link), and no additional treatment was performed in the ND group. The cells were subsequently treated with liraglutide (200 nM; HY-P0014, MCE) + NaCl (80 mM) for 48 h to study the role of GLP-1R.
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