The serum concentration of insulin was measured by the ADVIA Centaur Immunoassay system (Siemens, USA), using a chemiluminescent immunoassay. Serum adipokines were determined by sandwich ELISA assays on the automated ELISA analyser Triturus (Grifols, USA), with the kits produced by Mediagnost, Germany (Adiponectin E09, sensitive leptin E077, resistin E50). Visfatin was determined by Visfatin C-Terminal Human, EIA Kit (Phoenix).
Triturus
Manufactured by Grifols
Sourced in Spain
Triturus is a laboratory equipment designed for automated ELISA (Enzyme-Linked Immunosorbent Assay) processing. It is capable of performing various liquid handling tasks, including sample and reagent dispensing, incubation, and washing steps, to facilitate the ELISA workflow.
Automatically generated - may contain errors
Lab products found in correlation
Advia centaur immunoassay system, by Siemens (1 mentions)
Architect i1000, by Abbott (1 mentions)
Sars cov 2 igg, by Abbott (1 mentions)
Innovance vwf ac, by Siemens (1 mentions)
Beckman access 2 immunoassay system, by Beckman Coulter (1 mentions)
Alinity hq, by Abbott (1 mentions)
Legend max human mrp8 14 elisa kit, by BioLegend (1 mentions)
Architect i2000sr, by Abbott (1 mentions)
6 protocols using triturus
1
Serum Biomarker Measurement Protocol
2
SARS-CoV-2 IgG Antibody Detection
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CLIA and/or ELISA-based tests have been applied to determine whether children have had SARS-CoV-2 infection. CLIA-based SARS-CoV-2 IgG (Abbott, Illinois-USA) assay was performed on Architect i1000 (Abbott, Illinois-USA) device. ELISA-based SARS-CoV-2 IgG (Vircell, Granada-Spain) assay was performed on Triturus (Grifols, Barcelona-Spain) device. In the CLIA-based test, index values above 1.4 S/C are positive, while values below 1.4 S/C are negative. In the ELISA-based test, antibody index values above 6 were determined as positive, values below 4 determined as negative, and values between them were determined as equivocal.
3
Venous Blood Sampling and Biomarker Analysis
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Within the following 24 h after admission, venous blood samples were drawn from all patients and centrifuged at 1200 × g for 15 min within 2 h of collection and subsequently stored at −80°C for further analysis. VWF activity (Innovance VWFAc, Siemens, Spain), MMP-10 levels (R&D Systems, USA), and TAFI activity (TAFIa, STA STACHROM TAFI, Stago, France) were measured with an automated ELISA analyzer TRITURUS (Grifols, Spain) in citrated plasma samples after being thawed on ice and thoroughly vortexed. The detection limit of the assays was 2.2%, 15.1 pg/ml, and 5% for VWFAc, MMP-10, and TAFIa, respectively. All experiments were performed and analyzed in a blinded manner.
4
Biomarkers of Bone and Mineral Metabolism
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Blood samples were taken from all subjects and routine analyses performed which included full blood count, blood urea, glucose, glycosylated haemoglobin (HbA1c), serum creatinine, albumin, calcium, phosphate and lipid profiles. Other samples for specified analysis including parathyroid hormone (PTH), osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL) and matrix Gla protein (MGP) were isolated in Z serum sep clot activator VACUTTE® by centrifugation at 2000 g for 15 min at 4°C and stored at −80°C until analysis. Serum levels of PTH were measured using the Beckman Access® 2 immunoassay system (Beckman Coulter, High Wycombe, U.K.). Assays for OPG and RANKL were performed using the automated enzyme-linked immunosorbent assay (ELISA) analyzer, Triturus® (Grifols, Cambridge, U.K.). MGP was measured using the BIOMEDICA GRUPEE Kit (BI-20062). All analyses were performed in duplicate.
5
Biomarkers in Hemodialysis and Peritoneal Dialysis
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Demographic data (e.g. gender, age, start date of dialysis treatment, comorbidities, systolic and diastolic blood pressure and body mass index) and laboratory data (creatinine, C-Reactive Protein -CRP, albumin -A, calcium -Ca, phosphorus -P, Parathyroid hormone -PTH, total cholesterol, low-density lipoprotein -LDL, haemoglobin -Hb) of both groups of patients were analyzed. In order to determine the levels of inflammatory biomarkers (FGF-23, soluble alpha klotho, indoxyl sulphate, osteocalcin, sclerostin, PINP, beta-CrossLaps), the blood samples of hemodialysis patients were collected before the second hemodialysis during a mid-week session, and the blood samples of peritoneal dialysis patients were collected before morning changes. These blood samples were stored at -80°C. Human FGF-23, sclerostin, osteocalcin, indoxyl sulfate, Procollagen 1 N Terminal Propeptide, soluble alpha klotho and Beta-CrossLaps ELISA kits were used to determine the molecular levels (supplied by Hangzhou Eastbiopharm Co., Ltd. -PRC, China). Due to the manufacturer instructions, 40 U/L of blood sample were collected from each volunteer and tested with micro-ELISA method. Results were identified with Triturus (Grifols) ELISA instrument with a 450-nm wavelength.
6
Biomarkers in Emergency Department
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Venous blood samples were drawn from all patients in the emergency department before the initiation of any treatment as standard-of-care and neutrophils, lymphocytes (Alinity hq, Abbott, USA), and serum glucose (Architect i2000SR, Abbott, USA) were measured by standard laboratory techniques. After receiving or not treatment, new blood samples were taken within the following 24h and C-reactive protein (CRP) was measured with an autoanalyzer (Architect i2000SR, Abbott, USA). These second samples were centrifuged at 1200 xg for 15 minutes, 4 ºC within 2 hours of collection and stored at -80°C for further analysis. Citrated plasma samples were thawed on ice and thoroughly vortexed before measuring calprotectin levels (LEGEND MAX Human MRP8/14 ELISA Kit, BioLegend, USA) with an automated ELISA analyzer TRITURUS (Grifols, Spain). The inter-and intra-assay variability averages were 7.4% and 2.8% respectively. All experiments were performed in a blinded manner following manufacturer´s instructions.
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