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3 protocols using atrazine

1

Analytical Methods for Environmental Samples

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Amoxicillin trihydrate, triclosan (99%), and estrone (99+%) were purchased from Alfa Aesar (Ward Hill, MA, USA), while acetaminophen (≥98%) and atrazine (≥97%) were procured from Tokyo Chemical Industry (TCI, Chuo-ku, Tokyo, Japan). Liquid phenol, sodium nitroprusside dihydrate (≥98%), sodium hydroxide, sodium hypochlorite and ethanol were purchased from VWR (Radnor, PA, USA) and used as received with no further purification. HPLC grade acetonitrile from EMD Millipore (Bedford, MA, USA) and deionized (DI) water (Milli-Q, 18.2 MΩ cm) were employed as the mobile phase for HPLC analysis. Chemical oxygen demand (COD) kits were purchased from CHEMetrics (Midland, VA, USA) with a range of 0–1500 ppm. Nitrate nitrogen (NO3-N) detection kits were purchased from HACH (Loveland, CO, USA).
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Atrazine Degradation Metabolite Analysis

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Atrazine (≥97%) was purchased from Tokyo Chemical Industry (TCI) (Chuo-ku, Tokyo). Liquid phenol, sodium nitroprusside dihydrate (≥98%), sodium hydroxide, sodium hypochlorite, sodium bicarbonate, ammonium acetate, magnesium sulphate, calcium chloride, ammonium nitrate, and ethanol were purchased from VWR (Radnor, PA, USA) and used as received with no further purification. Monopotassium phosphate, dipotassium phosphate, zinc sulfate, manganese sulfate, magnesium sulfate, ferric chloride, and yeast extract were purchased from Sigma-Aldrich (St Louis, MO, USA). HPLC-grade acetonitrile from EMD Millipore (Bedford, MA, USA) and de-ionized (DI) water (Milli-Q, 18.2 MΩ cm) were employed as the mobile phase for HPLC analysis. Chemical oxygen demand (COD) kits were purchased from CHEMetrics (Midland, VA, USA) with a range of 0–1500 ppm. Total ammonia nitrogen (TAN) was determined using an established method [23 (link)] with ±0.02 mg/L accuracy. Nitrate nitrogen (NO3-N) detection kits were purchased from HACH (Loveland, CO, USA).
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3

Fungal Biodegradation of Pesticides

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The brown-rot fungus Gloeophyllum trabeum KU-41 (NBRC 111644), the white-rot fungi P. chrysosporium ME-446 (ATCC 34541), Phanerochaete sordida YK-624 (ATCC 90872), and new isolates Ceriporia lacerata K-70 and Trametes versicolor K-41, which were isolated from natural decayed wood samples obtained from a mixed forest in Shizuoka, Japan, were used. These isolates were identified based on their 18S rRNA gene ITS and sequences (accession numbers: LC312412 and LC312413 for C. lacerate K-70; LC312414 and LC312415 for T. versicolor K-41).
Diuron was purchased from Tokyo Chemical Industry Co., Ltd., Japan. DCPU and 3,4-DCA were purchased from Wako Pure Chemical Industries, Ltd., Japan. All reagents had > 98% purity, and were used without further purification. Atrazine (Tokyo Chemical Industry) was used as an internal standard.
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