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Pp1g antibodies

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PP1g antibodies are specific monoclonal antibodies that recognize the protein phosphatase 1 gamma (PP1g) enzyme. PP1g is a serine/threonine-specific protein phosphatase that plays a crucial role in various cellular processes. The PP1g antibodies can be used to detect and study the expression and localization of PP1g in biological samples.

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Lab products found in correlation

Hrp conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Gfp antibody, by Proteintech (2 mentions) Aspp2, by Abcam (2 mentions) Quickhange mutagenesis, by Agilent Technologies (2 mentions) Repoman, by Abcam (2 mentions)

2 protocols using pp1g antibodies

1

GFP-tagged Protein Phosphatase Constructs

Cited 1 time
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The pEGFP(C1)-PP1α and pEGFP(C1)-PP1γ plasmids were previously described14 (link) and are available through Addgene (plasmids #44224 and #44225). The C-terminal truncation mutant pEGFP(C1)-PP1γΔ299–323 was derived from the full-length construct by PCR-based insertion of a premature STOP codon. The C-terminal chimera pEGFP(C1)-PP1γ(1–298)PP1α(299–330) was derived by PCR-based insertion of a SalI restriction site into the full-length construct at the relevant site, followed by digestion and ligation of the PP1α C-terminal coding sequence. The pEGFP(C1)-RRP1B and pEGFP(C1)-RRP1BRAxA (V684A/F686A) plasmids were previously described.15 (link) PP1 and RRP1B point mutants were derived using Quickhange mutagenesis (Agilent Technologies) and sequence confirmed. Antibodies that recognize RRP1B, RepoMan and ASPP2 were obtained from Abcam, PP1g antibodies were from Santa Cruz and GFP-antibodies from proteintech®. HRP-conjugated secondary antibodies were from ThermoFisher.
Srivastava G., Bajaj R., Kumar G.S., Gaudreau-Lapierre A., Nicolas H., Chamousset D., Kreitler D., Peti W., Trinkle-Mulcahy L, & Page R. (2022). The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs. Cell reports, 41(9), 111726.
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2

GFP-tagged Protein Phosphatase Constructs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The pEGFP(C1)-PP1α and pEGFP(C1)-PP1γ plasmids were previously described14 (link) and are available through Addgene (plasmids #44224 and #44225). The C-terminal truncation mutant pEGFP(C1)-PP1γΔ299–323 was derived from the full-length construct by PCR-based insertion of a premature STOP codon. The C-terminal chimera pEGFP(C1)-PP1γ(1–298)PP1α(299–330) was derived by PCR-based insertion of a SalI restriction site into the full-length construct at the relevant site, followed by digestion and ligation of the PP1α C-terminal coding sequence. The pEGFP(C1)-RRP1B and pEGFP(C1)-RRP1BRAxA (V684A/F686A) plasmids were previously described.15 (link) PP1 and RRP1B point mutants were derived using Quickhange mutagenesis (Agilent Technologies) and sequence confirmed. Antibodies that recognize RRP1B, RepoMan and ASPP2 were obtained from Abcam, PP1g antibodies were from Santa Cruz and GFP-antibodies from proteintech®. HRP-conjugated secondary antibodies were from ThermoFisher.
Srivastava G., Bajaj R., Kumar G.S., Gaudreau-Lapierre A., Nicolas H., Chamousset D., Kreitler D., Peti W., Trinkle-Mulcahy L, & Page R. (2022). The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs. Cell reports, 41(9), 111726.
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