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Spectra system uv6000lp

Manufactured by Thermo Fisher Scientific
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The Spectra System UV6000LP is a single-beam ultraviolet-visible (UV-Vis) spectrophotometer designed for routine laboratory analysis. It features a wavelength range of 190 to 1100 nanometers and a spectral bandwidth of 1.0 nanometer. The device is capable of performing photometric measurements with a photometric range of -0.3 to 3.0 Absorbance.

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Lab products found in correlation

Spectra system p2000, by Thermo Fisher Scientific (2 mentions) Supelcosil lc 18 column, by Merck Group (1 mentions) Millipore cellulose acetate filters, by Merck Group (1 mentions) Spectra system scm1000, by Thermo Fisher Scientific (1 mentions) Excalibur v 2, by Thermo Fisher Scientific (1 mentions) Centrifuge 5804 r, by Eppendorf (1 mentions) Rotavapor r 210, by Büchi (1 mentions) L 2000, by Hitachi (1 mentions) Toc 5 series, by Shimadzu (1 mentions)

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Spectra System (14 citations) Spectra System UV6000LP Diode Array Detector (2 citations)

5 protocols using spectra system uv6000lp

1

GFP Renaturation Reaction Analysis

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Samples of the GFP renaturation reaction mixture (containing 1 μM Hsp104 D484K and optionally 2 μM Ssa1 and 0.5 μM Ydj1) were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) according to (Smolenski et al., 1990 (link)) on GBC 1150 HPLC Pump, Spectra System AS3000 autosampler, Thermo Finnigan Spectra System UV6000LP. The separation was performed on 50 x 4,6 mm HyperClone 3u BDS C18, 130 A column (Phenomenex).
Kłosowska A., Chamera T, & Liberek K. (2016). Adenosine diphosphate restricts the protein remodeling activity of the Hsp104 chaperone to Hsp70 assisted disaggregation. eLife, 5, e15159.
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2

Quantification of Ginger and Curcumin in Oils

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The 6-gingerol, 6-shogaol and curcumin were HPLC-quantified in the phenolic extracts of oil samples through a Thermo Finnigan SpectraSystem UV6000LP HPLC system (Thermo Finnigan, San Jose, CA, USA) with diode-array detector and a Supelcosil LC-18 column (Sigma-Aldrich). The samples were injected into the column after filtering with 0.22 µm Millipore cellulose acetate filters (Merck Millipore, Billerica, MA, USA).
The 6-gingerol and 6-shogaol were HPLC-detected as follows [31] (link) C; chromatographic run time of 70 min. The content of each compound was expressed as milligram per gram of oil (mg/g).
Tinello F., Zannoni S., & Lante A. (2020). Antioxidant Properties of Soybean Oil Supplemented with Ginger and Turmeric Powders. Applied Sciences, 10(23), 8438-8438.
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3

HPLC Parabens Quantification Protocol

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The method has been described and validated by Tartaglia et al. [4 (link)]. Briefly, analyses were performed using an HPLC Thermo Fisher Scientific liquid chromatography system (Model: Spectra System P2000) coupled to a photodiode array detector (PDA) Model: Spectra System UV6000LP. Mobile phase was directly on-line degassed by using a Spectra System SCM1000 (Thermo Fisher Scientific, Waltham, MA, USA). Excalibur v.2.0 software (Thermo Fisher Scientific, Waltham, MA, USA) was used to collect and analyze data. Spherisorb C18 (15 cm × 4.6 mm, 5 µm) was used to resolve all parabens; the column was thermostated at 27 °C (± 1 °C) using a Jetstream2 Plus column oven during the analysis. The chromatographic separation was conducted in isocratic elution using phosphate buffer (28 mM, pH = 2.5) as solvent A and methanol as solvent B in volume percentages of 55 and 45, respectively. The flow rate was set at 1 mL/min. All the compounds were detected at the maximum wavelengths of 257 nm with retention time of 3.97, 6.00, 8.83, 10.43, 18.37, 19.75, and 22.33 min (for MPB, EPB, iPPB, PPB, iBPB, BPB, and BzPB, respectively).
Tartaglia A., Kabir A., Ulusoy S., Ulusoy H.I., Merone G.M., Savini F., D’Ovidio C., de Grazia U., Gabrielli S., Maroni F., Bruni P., Croce F., Melucci D., Furton K.G, & Locatelli M. (2019). Novel MIPs-Parabens based SPE Stationary Phases Characterization and Application. Molecules, 24(18), 3334.
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4

Analytical Methods for Nanomaterial Characterization

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The Vötsch Industrietechnik heating chamber, VC2033 mit TC-Steuerung; Centrifuge 5804 R from Eppendorf (Hamburg, Germany); Rotavapor R-210 from Buchi (Meierseggstrasse, Switzerland; magnetic stirrer (HTS 1003, LMS, Tokyo, Japan); UV-vis spectrophotometer, Hitachi L-2000 (Hitachi High Technology, Tokyo, Japan); Millipore® water system (18.2 Ω cm at 25 °C) was purchased from Millipore—Direct Q3 UV System equipment (Molsheim, France); Delsa Nano C (Coulter, CA, USA); scanning electron microscopy (SEM 5200LV, JEOL, Tokyo, Japan) to evaluate morphology. A HPLC-DAD system (SpectraSystem, Thermo, Darmstadt, Germany) equipped with a binary gradient pump (SpectraSystem P2000, Thermo, Darmstadt, Germany), automatic sampler (SpectraSystem AS1000, Thermo, Darmstadt, Germany), diode array detector (SpectraSystem UV6000LP, Thermo, Darmstadt, Germany), UV controller (SpectraSystem SN4000, Thermo, Darmstadt, Germany) and software Xcalibur™ 2.0.6 (Thermo Fisher Scientific Corporation, Waltham, MA, USA) was used as well as an UHPLC AcquityTM (Waters, Milford MA, USA) equipped with a binary pump, an auto-sampler binary solvent manager, a column thermostatting system and a diode array detector (DAD).
Direito R., Reis C., Roque L., Gonçalves M., Sanches-Silva A., Gaspar M.M., Pinto R., Rocha J., Sepodes B., Rosário Bronze M, & Eduardo Figueira M. (2019). Phytosomes with Persimmon (Diospyros kaki L.) Extract: Preparation and Preliminary Demonstration of In Vivo Tolerability. Pharmaceutics, 11(6), 296.
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5

Detailed Emissions Measurement Protocol

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Gaseous and particulate emissions were measured in the chimneys according to the European standard EN-304. At laboratory scale, O2 and CO were measured using paramagnetic and infrared cells (Rosemount™ NGA 2000,Emersion, St. Louis, USA). Concentrations of total volatile organic compounds (TVOC) were determined with a Flame Ionization Detector (FID, Cosma graphite 55, Igny, France) and are reported as carbon equivalent (Table 1). The Water Soluble Organic Compound (WSOC) fraction was extracted from the PM using pure water and then quantified using a total organic carbon (TOC) analyzer (TOC-V Series, Shimadzu, Columbia, USA). To quantify the 16 PAHs recommended for analysis by the U.S. Environmental Protection Agency (EPA), we used an Accelerated Solvent Extractor (ASE 300, Dionex, Sunnyvale, USA) followed by High Performance Liquid Chromatography (HPLC) coupled with two detectors: a Diode Array Detector (DAD, Thermo Finnigan, Spectra System UV6000LP, Waltham, USA) and a fluorescence detector (Thermo Scientific, Finnigan Surveyor FL Plus, USA), following an analytical method (HPLC-FL-DAD) that was recently published (Liaud et al., 2015 ).
Arif A.T., Maschowski C., Garra P., Garcia-Käufer M., Petithory T., Trouvé G., Dieterlen A., Mersch-Sundermann V., Khanaqa P., Nazarenko I., Gminski R, & Gieré R. (2017). Cytotoxic and genotoxic responses of human lung cells to combustion smoke particles of Miscanthus straw, softwood and beech wood chips. Atmospheric environment (Oxford, England : 1994), 163, 138-154.
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