Aβ25 35 fragment

Manufactured by Merck Group

Sourced in United States

Aβ25–35 fragment is a synthetic peptide that corresponds to a specific region of the amyloid-beta (Aβ) protein. It is commonly used in research applications, particularly in the study of Alzheimer's disease and related neurodegenerative conditions.

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Lab products found in correlation

Ab150160, by Abcam (1 mentions) Nbp2 13075, by Novus Biologicals (1 mentions) Nb100 1028, by Novus Biologicals (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Cleaved poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Catalase, by Merck Group (1 mentions) Superoxide dismutase sod from bovine erythrocytes, by Merck Group (1 mentions) Diethylenetriaminepentaacetic acid dtpa, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Thioflavin t, by Merck Group (1 mentions)

3 protocols using aβ25 35 fragment

Immunofluorescence Localization of Aβ, ICAM-1, and Iba1

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The following main reagents were used: Aβ25–35 fragment (Sigma, United States), CEF (Roche, Switzerland), a mouse monoclonal antibody to Aβ (cat. # NBP2-13075, 1:1,000 dilution; Novus Biologicals, United States), a rat monoclonal antibody to CD54/ICAM-1 (cat. # 16-0542-81, 1:300 dilution; eBioscience, Thermo Fisher Scientific, United States), a goat polyclonal antibody to microglial marker AIF-1/IBA1 (cat. # NB100-1028, 1:200 dilution; Novus Biologicals, United States), an Alexa Fluor 568-conjugated goat anti-mouse IgG polyclonal antibody (cat. # ab175473, 1:400 dilution, Abcam, United Kingdom), an Alexa Fluor 594-conjugated goat anti-rat IgG polyclonal antibody (cat. # ab150160, 1:500 dilution; Abcam, United Kingdom), and an Alexa Fluor 488-conjugated donkey anti-goat IgG polyclonal antibody (cat. # ab150129, 1:200 dilution; Abcam, United Kingdom).

Tikhonova M.A., Amstislavskaya T.G., Ho Y.J., Akopyan A.A., Tenditnik M.V., Ovsyukova M.V., Bashirzade A.A., Dubrovina N.I, & Aftanas L.I. (2021). Neuroprotective Effects of Ceftriaxone Involve the Reduction of Aβ Burden and Neuroinflammatory Response in a Mouse Model of Alzheimer’s Disease. Frontiers in Neuroscience, 15, 736786.

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Aβ25–35 Induced Apoptosis and Signaling

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The Aβ_25–35 fragment and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical Co (St Louis, MO, USA), and the Annexin V-FITC Apoptosis Detection kit was obtained from BD Biosciences (San Jose, CA, USA). Antibodies for p-ATM, p-ATR, p-Chk1, p-Chk2, cleaved poly (ADP ribose) polymerase (PARP), and Caspase-3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against beta-site APP-cleaving enzyme 1 (BACE), and CD10 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for APP and γ-H2AX, were purchased from Millipore (Bedford, MA, USA), and low density lipoprotein receptor–related protein 1 (LRP1) and A disintegrin and metalloproteinase 10 (ADAM10) were purchased from Abcam (Cambridge, MA, USA).

Lee J.S., Kim J.Y., Kim H.J., Kim J.C., Lee J.S., Kim N, & Park M.J. (2016). Effects of combined radiofrequency field exposure on amyloid-beta–induced cytotoxicity in HT22 mouse hippocampal neurones. Journal of Radiation Research, 57(6), 620-626.

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Amyloid-beta Oxidative Damage Assay

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Aβ_25-35 fragment (NH₂- and COOH-terminal free peptide), superoxide dismutase (SOD) from bovine erythrocytes (EC 1.15.1.1), catalase, diethylenetriaminepentaacetic acid (DTPA), and Thioflavin-T were obtained from Sigma (St. Louis, MO, USA). l-methionine and hydrogen peroxide (30%) were purchased from Fluka (Buchs, Switzerland). All other chemicals were of the highest purity commercially available. H₂O₂ concentration was verified using UV absorption at 240 nm (ε = 43.6 M⁻¹cm⁻¹) [60 (link)]. All solutions and buffers were prepared with distilled water purified in a Millipore (Burlington, MA, USA) Milli-Q system and contained DTPA to avoid metal-dependent oxidative reactions.

Francioso A., Baseggio Conrado A., Blarzino C., Foppoli C., Montanari E., Dinarelli S., Giorgi A., Mosca L, & Fontana M. (2020). One- and Two-Electron Oxidations of β-Amyloid25-35 by Carbonate Radical Anion (CO3•−) and Peroxymonocarbonate (HCO4−): Role of Sulfur in Radical Reactions and Peptide Aggregation. Molecules, 25(4), 961.

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