Placenta homogenates were centrifuged for 5 min at 12000 g at 4°C and the supernatant was transferred (taking care to avoid the pellet and any fat) to a fresh Eppendorf tube and stored at −20°C until analysis. Supernatants were thawed on ice, centrifuged again at 4°C to remove any remaining particulates and the supernatant collected. Cytokine/chemokine levels were measured using the Bio-Plex-Pro Mouse Cytokine 23-Plex Panel multiplex cytokine bead kit (Bio-Rad), which quantifies 23 different cytokines/chemokines. The assays were performed according to manufacturer instructions, except using 10-fold less standard and half the number of coupled beads and detection antibodies indicated in the protocol.
Bio plex pro mouse cytokine 23 plex panel multiplex cytokine bead kit
Manufactured by Bio-Rad
The Bio-Plex Pro Mouse Cytokine 23-Plex Panel is a multiplex cytokine bead kit that allows for the simultaneous quantification of 23 different mouse cytokines in a single sample. The kit utilizes Luminex xMAP technology to enable the detection and measurement of these cytokines.
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Lab products found in correlation
3 protocols using bio plex pro mouse cytokine 23 plex panel multiplex cytokine bead kit
1
Placental Cytokine Profiling Protocol
2
Multiplex Cytokine Profiling in Tissue Homogenates
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue homogenate supernatants collected as described above were thawed on ice, centrifuged again at 4°C to remove any remaining particulates and then cytokine expression was measured using the Bio-Plex-Pro Mouse Cytokine 23-Plex Panel multiplex cytokine bead kit (Bio-Rad), which quantifies 23 different cytokines/chemokines. Assay was performed according to manufacturer instructions, except using 10-fold less standard and half the amount of coupled beads and detection antibodies indicated in the protocol.
3
Multiplex Cytokine Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue homogenate supernatants collected as described above were thawed on ice, centrifuged again at 4°C to remove any remaining particulates. Cytokine content was measured using the Bio-Plex-Pro Mouse Cytokine 23-Plex Panel multiplex cytokine bead kit (Bio-Rad), which quantifies 23 different cytokines and chemokines. The assay was performed according to manufacturer instructions, except using tenfold less standard and half the amount of coupled beads and detection antibodies indicated in the protocol.
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