P cd79a

Manufactured by Cell Signaling Technology

Sourced in United States

P-CD79A is an antibody that detects the phosphorylated form of the CD79A protein. CD79A is a component of the B-cell antigen receptor complex and plays a role in B-cell activation and signaling.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

CD79A (3 citations)

2 protocols using p cd79a

Western Blot Analysis of BCR Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luxeptinib was provided by Aptose Biosciences. Ibrutinib (#HY-10997) was purchased from MedChem Express. Goat anti-human IgM (#109-005-043) was purchased from Jackson ImmunoResearch. Human lymphoma cell lines SU-DHL-6 (#CRL-2959), JeKo-1 (#CRL-3006), RL (#CRL-2261) and Fetal Bovine Serum (#30–2020) were obtained from ATCC. SU-DHL-6 is a human lymphoblast-like cell line, JeKo-1 is a mantle cell lymphoma cell line and RL is a human non-Hodgkin’s lymphoma B cell line. RPMI-1640 medium (#11875–093) and penicillin/streptomycin (#15070063) were purchased from Thermo Fisher Scientific. Antibodies against p-BTK (Y223) (#87141), BTK (#56044), p-PLCγ2 (#3871), p-SYK (#2710), SYK (#80460), p-BLNK (#3601), BLNK (#36438), p-CD79A (#5173), p-LYN/LCK/HCK/BLK (#70926), p-LYN (Y507) (#2731), LYN (#2796, #4576), pSrc family (#6943) and GAPDH (#2118) were purchased from Cell Signaling Technology. Phospho-BTK (Y551) (#ab40770) was purchased from Abcam. PLCγ2 (#sc-5283) and CD79A (#sc-20064) were purchased from Santa Cruz Biotechnology. All other reagents and chemicals used were of analytical grade and were obtained from Sigma-Aldrich or Thermo Fisher Scientific.

Sonowal H., Rice W.G, & Howell S.B. (2023). Luxeptinib interferes with LYN-mediated activation of SYK and modulates BCR signaling in lymphoma. PLOS ONE, 18(3), e0277003.

+ Open protocol

+ Expand

Phosphoflow Analysis of B Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

For phosphoflow experiments, 0.5 × 10⁶ cells were cultured in RPMI‐2% FCS at 37°C for 1 min for pPI3K p85 (Cell Signaling Technologies), 3 min for pSHP‐1 (Cell Signaling Technologies), 5 min for pSrc (Abcam), pLyn (Abwiz Bio, San Diego, CA, USA), pCD79a (Cell Signaling Technologies), pSyk, pSLP‐65, pPLCγ2, and pErk (all BD Biosciences) or for 3 h for pS6 and pAkt (both Cell Signaling Technologies) with 25 μg/mL anti‐IgM or antigen‐binding F(ab’)₂‐Igκ fragments (anti‐IgK; Jackson Immunoresearch). After fixation with the eBioscience FoxP3 staining kit Fix/Perm solution, cells were washed twice with the accompanying Perm/Wash solution (Invitrogen). Next, cells were incubated with varying combinations of monoclonal antibodies (Supporting information Table S1) and stained according to previously described procedures [31 (link)]. After staining for the phosphoproteins, samples were measured in MACS buffer on an LSR‐II (BD Biosciences) and analyzed using FlowJo v10 (BD Biosciences).

Rip J., de Bruijn M.J., Neys S.F., Singh S.P., Willar J., van Hulst J.A., Hendriks R.W, & Corneth O.B. (2021). Bruton's tyrosine kinase inhibition induces rewiring of proximal and distal B‐cell receptor signaling in mice. European Journal of Immunology, 51(9), 2251-2265.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!