Ab77735

Manufactured by Abcam

Ab77735 is a primary antibody product. It is designed for use in immunoassay applications. Further details about the specific function or intended use are not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab155091, by Abcam (3 mentions) Ab183727, by Abcam (3 mentions) Ab3329, by Abcam (3 mentions) Ab4389, by Abcam (2 mentions) Ab3328, by Abcam (2 mentions) Sc 47197 n 17, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated 3f10, by Merck Group (2 mentions) Infrared imaging technology, by LI COR (2 mentions) Ab18197, by Abcam (1 mentions) Ang 2 a9525, by Merck Group (1 mentions) Tunel assay kit, by Promega (1 mentions) α sma, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Santa Cruz Biotechnology (1 mentions) Ab90867, by Abcam (1 mentions) Ab7291, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Mini protean transfer system, by Bio-Rad (1 mentions)

4 protocols using ab77735

Immunoblotting Analysis of Immune Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with PBS, lysed in sample buffer, boiled for 3 min, resolved by SDS-PAGE and analyzed by immunoblotting using primary antibodies specific for human MX2 (sc-47197 (N-17), Santa Cruz Biotechnology), human TRIM5α (ab4389, Abcam), human PSMB8 (ab3329, Abcam), human PSMB9 (ab3328, Abcam), human PSMB10 (ab77735, Abcam), human PA28A (ab155091, Abcam), human PA28B (ab183727, Abcam), FLAG (HRP-conjugated M2, Sigma), HA (HRP-conjugated 3F10, Sigma) or human α-tubulin (DM1A, Sigma), and detected using either horseradish peroxidase-conjugated secondary antibodies and chemiluminescence (ECL⁺ western blotting substrate, Pierce) or IRDye®-800CW-labelled secondary antibodies and the LI-COR infrared imaging technology (LI-COR UK LTD).

Jimenez-Guardeño J.M., Apolonia L., Betancor G, & Malim M.H. (2019). Immunoproteasome activation enables human TRIM5α restriction of HIV-1. Nature microbiology, 4(6), 933-940.

+ Open protocol

+ Expand

Immunofluorescent Analysis of Proteasome Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies to β2i (ab77735), β2 (ab22650), and α-smooth muscle actin (α-SMA) (ab5694) were purchased from Abcam Inc. Antibodies to Mac-2 (sc-20157), myocardin (sc-33766), GAPDH (sc-365062) and 4′,6-diamidino-2-phenylindole (DAPI) were from Santa Cruz Biotechnology. Antibodies to CD4 (NBP1-19371) were from Novus. All antibodies were used in a 1:200 dilution. For immunofluorescent staining, antibodies to β5i (ab3329), pCNA (ab18197) and α-SMA were from Abcam Inc. Anti-mouse or anti-rabbit conjugated antibodies were from Cell Signaling Technology. TUNEL assay kit was from Promega. For Flow Cytometry, antibodies to CD45 (557659) was from BD Bioscience Pharmingen. Proteasome inhibitor bortezomib (BZT, sc-217785A) was purchased from Santa Cruz Biotechnology. Ang II (A9525) was from Sigma.

Ren H., Li F., Tian C., Nie H., Wang L., Li H.H, & Zheng Y. (2015). Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E−/− Mice. Scientific Reports, 5, 15730.

+ Open protocol

+ Expand

Immunoblotting Analysis of Immune Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Jimenez-Guardeño J.M., Apolonia L., Betancor G, & Malim M.H. (2019). Immunoproteasome activation enables human TRIM5α restriction of HIV-1. Nature microbiology, 4(6), 933-940.

+ Open protocol

+ Expand

Proteasome Subunit Quantification by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were transferred to PVDF membranes using a Bio-Rad mini-protean transfer system containing a buffer of 25 mM Tris base, 192 mM glycine, 0.1% SDS. Proteins were transferred for 6h at 40V at 4°C. Membranes with transferred proteins were blocked with a 1:1 mixture of Li-COR blocking buffer and PBS. Membranes were then incubated in blocking solution for 1 h and primary Abs (1:1000) applied for 16 h at 4°C. Membranes were washed and incubated with secondary Abs (IR Dye® 800CW goat anti-mouse IgG (H+L) or IR Dye 680CW donkey anti-rabbit IgG (H+L), and imaged using an Odyssey Li-Cor platform. Antibodies used were rabbit anti-PSMB5 polyclonal (Abcam, ab90867), rabbit monoclonal to anti-PSME1 (EPR10967, Abcam, ab155091), rabbit monoclonal to anti-PSME2 (EPR14931, Abcam, ab183727), rabbit anti-LMP2 polyclonal (PSMB9, Abcam, ab42987), rabbit anti-PSMB10 polyclonal (Abcam, ab77735), rabbit anti-LMP7 monoclonal (EPR14482, PSMB8, Abcam, ab180606), rabbit anti-PSMA6 polyclonal (Cell Signaling 2459), rabbit anti-PSMB1 polyclonal (Abcam, ab196623), rabbit anti-PSMB2 polyclonal (Abcam, ab140426), mouse anti-Rpn5 monoclonal (PSMD12, clone H3, Santa Cruz Biotechnology, sc-398279), mouse anti-α-tubulin monoclonal (DM1a, Abcam, ab7291), and rabbit anti-histone 3 polyclonal (Abcam, ab1791).

Woodle E.S., Tremblay S., Brailey P., Girnita A., Alloway R.R., Aronow B., Dasgupta N., Ebstein F., Kloetzel P.M., Lee M.J., Kim K.B., Singh H, & Driscoll J.J. (2019). Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 20(2), 399-410.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!