Total internal reflection fluorescence (TIRF) microscopy experiments in this study were performed on an Axio Observer Z1 microscope (Carl Zeiss Microscopy GmbH, Jena, Germany), equipped with 488 nm/532 nm / 642 nm laser lines (Omicron-Laserage, Rodgau-Dudenhofen, Germany) and an iXon X3 EMCCD (Andor Technology, Belfast, UK). Image data were acquired using an alpha Plan-Apochromat 63x/1.46 Oil objective (Zeiss) with a 1.6-fold auxiliary magnification - achieving a final pixel size of 159 ± 3 nm. For temperature control, the objective was equipped with a water-supplied heating-ring connected to a thermostatic circulator (F-25-MC, Julabo, Seelbach, Germany).
F 25 mc
Manufactured by Julabo
Sourced in Germany
The Julabo F-25-MC is a laboratory recirculating cooler designed to provide precise temperature control. It features a digital temperature display, adjustable temperature range, and a durable stainless steel bath. The core function of the F-25-MC is to accurately regulate the temperature of liquids or samples within a laboratory environment.
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Lab products found in correlation
2 protocols using f 25 mc
1
TIRF Microscopy for Live-Cell Imaging
2
Single-Molecule TIRF Microscopy Experiments
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TIRF microscopy experiments in this study were performed on an Axio Observer Z1 microscope (Carl Zeiss Microscopy GmbH, Jena, Germany) equipped with 488/532/642 nm laser lines (Omicron-Laserage, Rodgau-Dudenhofen, Germany) and an iXon X3 EMCCD (Andor Technology, Belfast, UK). Image data were acquired using an alpha Plan-Apochromat 63×/1.46 Oil objective (Zeiss) with a 1.6-fold auxiliary magnification—achieving a final pixel size of 159 ± 3 nm. For temperature control, the objective was equipped with a water-supplied heating ring connected to a thermostatic circulator (F-25-MC; Julabo, Seelbach, Germany).
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