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Mouse monoclonal anti gfp antibody

Manufactured by Fujifilm
Sourced in United States

The Mouse monoclonal anti‐GFP antibody is a laboratory reagent used to detect and visualize green fluorescent protein (GFP) in various experimental systems. It is a purified antibody produced in mice that specifically binds to GFP, enabling researchers to identify and track GFP-labeled proteins or cells.

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2 protocols using mouse monoclonal anti gfp antibody

1

Periostin Isoform Detection Assay

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Growth factors were obtained from the following companies: TGF‐β1 (#240‐B‐010/CF), HGF (#294‐HG‐005/CF), and PDGF (#220‐BB‐010, R&D Systems, Inc.); basic FGF2 (#064–04541, FUJIFILM Wako Pure Chemical Corp.); and EGF (#100–47, Pepro Tech). We generated a polyclonal anti‐periostin antibody for detection exon 21 by using specific peptides (GHLFEDEEIKRLLQGC). For recognizing all periostin isoforms, we used an anti‐periostin antibody against N‐terminal of periostin (ab14041, Abcam). A mouse monoclonal anti‐GFP antibody and a mouse monoclonal β‐actin antibody were obtained from Wako and Sigma, respectively.
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2

Antibody Sources for Protein Analysis

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Antibodies were obtained from the following sources: mouse monoclonal anti‐GFP antibody from Wako; mouse anti‐β‐actin monoclonal antibody from Sigma; mouse anti‐Smad2/3 antibody from BD Transduction Laboratories (San Jose, CA, USA); rabbit polyclonal anti‐Smad4 and anti‐ALK5 antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti‐cleaved caspase‐3 antibody from Cell Signaling Technology (Danvers, MA USA); and rabbit polyclonal anti‐Ki‐67 antibody from Leica (Newcastle, UK). Rabbit polyclonal anti‐phosphorylated Smad2 (PS2) and anti‐Smad2 antibodies were prepared in‐house.12
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