FT-IR spectra were obtained from the KBr slice with a Nicolet iS10 FT-IR spectrophotometer (Thermo Fisher Scientific, Shanghai). X-ray diffraction (XRD) patterns were recorded on a Bruker D8 Advance diffractometer (Germany) with a Cu Kα (1.5406 Å) radiation source. All pH measurements were performed with a Sartorius PB-10 digital pH meter (Shanghai, China). Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were performed with a HITACHI model HT7700 instrument operating at 80 kV accelerating voltage and a ZEISS Gemini 300 with OXFORD Xplore, respectively. The ultraviolet-visible (UV-Vis) absorption spectra were recorded by using a U3900 spectrophotometer (Hitachi, Japan). All fluorescence measurements were performed on a Hitachi F-7000 fluorescence spectrometer. The excitation wavelength was set at 496 nm, and the emission spectra from 550 to 750 nm were observed. The fluorescence intensity at 610 nm was used to evaluate the performance of the proposed strategy.
Pb 10 digital ph meter
Manufactured by Sartorius
Sourced in China
The PB-10 digital pH meter is a compact and portable instrument designed for accurate pH measurement. It features a large, easy-to-read LCD display and provides pH readings with a resolution of 0.01 pH. The device is equipped with automatic temperature compensation to ensure reliable measurements across a wide range of temperatures. The PB-10 is powered by a rechargeable battery, making it suitable for use in various laboratory or field applications.
Automatically generated - may contain errors
Lab products found in correlation
Na2hpo4, by Tianjin Guangfu (3 mentions)
U 3900 spectrophotometer, by Hitachi (2 mentions)
Mercury yh 400 nmr spectrometer, by Agilent Technologies (2 mentions)
Nicolet is10 ftir spectrophotometer, by Thermo Fisher Scientific (1 mentions)
F 7000 fluorescence spectrometer, by Hitachi (1 mentions)
Ferrous chloride tetrahydrate, by Merck Group (1 mentions)
Vertex 70, by Shimadzu (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)
Nanosem 450, by Thermo Fisher Scientific (1 mentions)
Glucose, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Glucose oxidase, by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
Uv 2550 spectrophotometer, by Shimadzu (1 mentions)
Q trap 2000, by Thermo Fisher Scientific (1 mentions)
Nah2po4, by Beijing Chemical Works (1 mentions)
F 2700 fluorescence spectrometer, by Hitachi (1 mentions)
Agilent 1290 microtof qii, by Bruker (1 mentions)
Toluene, by Sinopharm (1 mentions)
Microflex lrf 20, by Bruker (1 mentions)
7 protocols using pb 10 digital ph meter
1
Multifunctional Material Characterization
2
Colorimetric Assay for Antibacterial Activity
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Ferrous chloride tetrahydrate (FeCl2·4H2O), gallic acid (GA), glucose oxidase (GOx), glucose, 3,3′,5,5′-tetramethylbenzidine (TMB), acetic acid (CH3COOH), H3PO4, and NaH2PO4 were purchased from Sigma-Aldrich; sucrose, fructose, lactose, sodium chloride (NaCl), potassium chloride (KCl), and sodium bicarbonate (NaHCO3) were purchased from Aladdin Chemistry Company. Hydrogen peroxidase (H2O2) was purchased from Alfa Aesar and the apple juice was bought from HuiYuan. The chemicals were all of analytical grade and were used in the original manner without further purification.
Scanning electron microscopy (SEM) was NanoSEM-450 (FEI, Brno, Czech Republic). Transmission electron microscopy (TEM) was JEM-1230 (Japanese Electronics, Tokyo, Japan), which adopts the accelerating voltage of 40–120 kV. The Fourier–transform infrared spectrometer (FITR) was purchased from Germany with the model Vertex70, the visible ultraviolet spectrophotometer were used a UV-2550 spectrophotometer (Shimadzu, Kyoto, Japan). The PB-10 digital pH meter (Sartorius, Goettingen, Germany) was used to test the pH values of the solution.
Strains: Escherichia coli (ATCC25922); Staphylococcus aureus (ATCC25923); Salmonella typhimurium (ATCC50115); Listeria monocytogenes (CMCC54004); and Enterobacter sakazalii (ATCC29544).
Scanning electron microscopy (SEM) was NanoSEM-450 (FEI, Brno, Czech Republic). Transmission electron microscopy (TEM) was JEM-1230 (Japanese Electronics, Tokyo, Japan), which adopts the accelerating voltage of 40–120 kV. The Fourier–transform infrared spectrometer (FITR) was purchased from Germany with the model Vertex70, the visible ultraviolet spectrophotometer were used a UV-2550 spectrophotometer (Shimadzu, Kyoto, Japan). The PB-10 digital pH meter (Sartorius, Goettingen, Germany) was used to test the pH values of the solution.
Strains: Escherichia coli (ATCC25922); Staphylococcus aureus (ATCC25923); Salmonella typhimurium (ATCC50115); Listeria monocytogenes (CMCC54004); and Enterobacter sakazalii (ATCC29544).
3
Characterization of Compounds by Analytical Techniques
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The nuclear magnetic resonance spectroscopy (NMR) of compounds were identified by 1 H NMR and 13 C NMR (Varian Mercury YH-400 NMR spectrometer), using tetramethylsilane (TMS) as an internal standard. ESI mass spectra were obtained using a Q-Trap 2000 (Applied Biosystems Corporation, USA) without using the liquid phase part. MOLDI-TOF mass spectra were obtained using a Autoflex speed TOF by Brucker. The average molecular weight and the molecular weight distribution was determined by GPC (Agilent 1260). All fluorescence measurements were carried out in a 1-cm path length quartz cuvette with a Hitachi F-2700 spectrometer (Shimadzu Corporation, Japan). Measurements of fluorescence quantum yields were carried out with an FLS920 steady state and transient state fluorescence spectrometer (Edinburgh Instrument). All pH measurements were tested with a Sartorius PB-10 digital pH meter. All the optical measurements were carried out at room temperature (298 K) under ambient conditions.
4
Fluorescent Detection of Metal Cations
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8-Aminoquinoline and phenylacetylene were purchased from Saan Chemical Technology (Shanghai) Co., Ltd. 2-Methoxybenzaldehyde was purchased from Shanghai Bi De Pharmaceutical Technology Co., Ltd. Toluene was obtained from Sinopharm Chemical Reagent Co., Ltd. TA-Au (Triazole Au(I)) catalyst was synthesized with Ref. 29. NaH2PO4 was produced at Beijing Chemical Plant. Na2HPO4 was produced by Tianjin Guangfu Fine Chemical Research Institute. Cationic interfering ion standard solutions (1 mmol/L) used in the experiment (Ag + , Pd 2+ , Pt 2+ , Co 2+ , Cr 6+ , Zn 2+ , Ba 2+ , Mg 2+ , Hg 2+ , Al 3+ , Ca 2+ , Ni 2+ , Mn 2+ , Cu 2+ , Na + , Be 2+ , Cd 2+ , Cs + , K + , Fe 3+ , V 3+ , Sr 2+ , Li + , Rb + , Pd 2+ and Au 3+ ) were obtained by diluting their nitrates with distilled water.
All fluorescence tests were performed on a F-2700 fluorescence spectrometer (Hitachi, Japan).
The nuclear magnetic resonance spectroscopy (NMR) of compounds was identified by 1 H NMR (Varian Mercury YH-400 NMR spectrometer), using tetramethylsilane (TMS) as an internal standard. ESI mass spectra were obtained using an Agilent1290-micrOTOF Q II (Bruker, Germany) without using the liquidphase part. MALDI-TOF mass spectra were obtained using a Bruker Microflex LRF20. All pH measurements were tested with a Sartorius PB-10 digital pH meter.
All fluorescence tests were performed on a F-2700 fluorescence spectrometer (Hitachi, Japan).
The nuclear magnetic resonance spectroscopy (NMR) of compounds was identified by 1 H NMR (Varian Mercury YH-400 NMR spectrometer), using tetramethylsilane (TMS) as an internal standard. ESI mass spectra were obtained using an Agilent1290-micrOTOF Q II (Bruker, Germany) without using the liquidphase part. MALDI-TOF mass spectra were obtained using a Bruker Microflex LRF20. All pH measurements were tested with a Sartorius PB-10 digital pH meter.
5
Fluorescence Spectroscopy Analysis
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Fluorescence tests were performed on a Gangdong technology F-380 fluorescence spectrometer (Tianjin, China) equipped with 1.0 cm micro quartz cells. The excitation wavelength was set at 340 nm and the fluorescence spectra were collected from 400 to 625 nm. A fluorescence intensity of 490 nm was used to evaluate the performance of the sensing system. The pH of solutions was adjusted by a PB-10 digital pH meter (Sartorius, Shanghai, China). The ultraviolet-visible absorption spectra were collected at room temperature on a U3900 spectrophotometer (Hitachi, Tokyo, Japan) equipped with 1.0 cm micro quartz cells.
6
UV-Vis Spectroscopy of Sample Absorbance
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The UV–vis absorption spectra were recorded on a U-3900H spectrophotometer (Hitachi, Japan) at room temperature. The pH measurements were carried out on a model PB-10 digital pH-meter (Sartorius Scientific Instruments Co., Ltd., Beijing, China). The value of absorbance (A) of the UV–vis absorption spectra was the average of five repeats and then used in this study without baseline correction, curve fitting, or normalization.
7
Soil Physicochemical Characterization Protocol
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The soil pH was measured in a 1:2.5 mixture of soil and deionized water using a Sartorius PB-10 digital pH meter. The soil moisture was calculated on 5 g fresh subsamples that had been dried in a 105 °C oven for 12 hr. The soil TOC was measured using a TOC analyzer (TOC-VCPH, Shimadzu, Japan), and the soil total nitrogen (TN) was detected using the Kjeldahl method with some modifications (Bremner, 1960) . The soil water-soluble organic carbon (WSOC) and nitrogen (WSON) were extracted using the methods developed by Jones and Willett (2006) and determined using a Shimadzu TOC-TN analyzer. The soil NH 4 + -N, NO 3 --N and NO 2 --N were determined using an autoanalyzer (Auto Analyzer 3, Bran Luebbe, Germany)
with extracts containing 0.05 M K 2 SO 4 (1: 4, soil: extractant).
with extracts containing 0.05 M K 2 SO 4 (1: 4, soil: extractant).
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