HUVEC cultured medium was collected after 30 minutes or 24 hours and TGF-β content was measured using TGF-beta 1 Emax ImmunoAssay System according to manufacturer´s instructions (Promega, Madison, WI). All the samples were not submitted to acidification step in order to detect just active TGFβ1. Proliferation assay was performed by BrdU incorporation using ELISA colorimetric assay kit according to manufacturer’s instructions (Roche Applied Science, Germany). Expression of critical proteins was determined by immunocytochemistry and Western blot.
Tgf beta 1 emax immunoassay system
Manufactured by Promega
Sourced in United States
The TGF-beta 1 Emax ImmunoAssay System is a laboratory equipment product designed to measure TGF-beta 1 levels in biological samples. It uses an enzyme-linked immunosorbent assay (ELISA) format to detect and quantify the presence of TGF-beta 1 in the samples.
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Lab products found in correlation
2 protocols using tgf beta 1 emax immunoassay system
1
TGF-β Quantification and Proliferation Assay
2
Analyzing Nuclear and Cytoplasmic IDO1 Signaling
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Nuclear and cytoplasmic extracts from Ido1−/− pDCs, transfected with WT and ITIM‐mutated Ido1 mRNA and incubated for 48 hr at 37°C, were prepared according the manufacturer's protocol for the TransAM Flexi NF‐κB Family Kit (Active Motif). Analysis of p52 and RelB expression was performed on normalized samples of pDCs using rabbit polyclonal anti‐p100/p52 (Cell Signaling Technology, MA, USA), anti‐RelB (Santa Cruz Biotechnology) antibodies. Anti–β‐tubulin and anti‐Lamin B1 (Thermo Fisher Scientific, Massachusetts, USA) were used as normalizers of the cytoplasmic and nuclear extracts respectively. TGF‐β production in culture supernatants from those cells was measured by means of TGF beta 1 Emax Immuno Assay System (Promega, Madison, WI 53711 USA).
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