Cell viability was measured using the CellTiter-Blue® Cell Viability Assay, which is a fluorometric method for estimating the number of viable cells based on the metabolic capacity of cells. On the day of analyzing cell viability, CellTiter-Blue reagent was added into culture wells at a ratio of 1:5 to the medium, and the plates were incubated for 2 to 4 h at 37°C in dark. After the incubation, fluorescence was measured using the Synergy 2 Microplate Reader (BioTek) and Gen5 3.03 Microplate Reader and Imager Software with an excitation wavelength of 530 nm and an emission wavelength of 590 nm.
Gen5 3.03 microplate reader and imager software
Manufactured by Agilent Technologies
Sourced in United States
Gen5 3.03 Microplate Reader and Imager Software is a software application designed to control Agilent's microplate reader and imager hardware. It provides functionalities for data acquisition, analysis, and reporting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gen5 3.03 microplate reader and imager software
1
Fluorometric Cell Viability Assay
2
Quantification of soluble PD-L1 in plasma
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Peripheral blood samples were collected before initiation of immunotherapy or chemotherapy. Blood samples were collected in tubes containing ethylenediaminetetraacetic acid (EDTA). The samples were then centrifuged for 10 min at 2,000 rpm. The obtained plasma was collected and stored at − 80 °C until the assay was performed. sPD-L1 concentration was determined using a Human PD-L1 ELISA Kit (Cat #BMS2327, ThermoFisher, Waltham, USA). The ELISA was performed according to the manufacturer’s instructions. Absorbance was measured at 450 nm using a BioTek ELx800 Absorbance Microplate Reader (BioTek, Winooski, VT, USA). A standard curve was generated, from which the concentrations were calculated. Analysis was performed using the Gen5 3.03 Microplate Reader and Imager Software (BioTek, Winooski, VT, USA).
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