Air mp

Manufactured by Nikon

Sourced in Japan

The Nikon AIR-MP is a compact and lightweight microscope power supply designed for laboratory use. It provides a stable and adjustable voltage output to power various microscope accessories and components. The AIR-MP is built with high-quality components to ensure reliable performance and long-term durability.

Automatically generated - may contain errors

Lab products found in correlation

Cm1950, by Leica camera (2 mentions) Ab16645, by Abcam (1 mentions) M8440, by Merck Group (1 mentions) G6642, by Merck Group (1 mentions) S21374, by Thermo Fisher Scientific (1 mentions) Tecnai g2 f20, by Thermo Fisher Scientific (1 mentions) Ag ic 50kn, by Shimadzu (1 mentions) Vs120, by Olympus (1 mentions) Mab377, by Merck Group (1 mentions) Alexa 488, by Abcam (1 mentions) Mouse anti neun, by Merck Group (1 mentions) Abe457, by Merck Group (1 mentions) Ab152, by Merck Group (1 mentions) R37117, by Thermo Fisher Scientific (1 mentions)

10 protocols using air mp

Multifaceted Neurochemical Profiling of Mouse Brain

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Mice were deeply anesthetized and transcardially perfused with phosphate-buffered saline (PBS), followed by 4% paraformaldehyde in PBS. For fixation, the brains were kept overnight in 4% paraformaldehyde. Each brain sample was placed in 30% sucrose in PBS for 48 h. After embedding and freezing, each brain was cut into 30 µm coronal slices using a cryostat (CM1950, Leica, Heidelberger, Germany).
For immunostaining, the brain slices were washed three times with PBS and incubated with primary antibodies (Foxp1: rabbit anti-Foxp1, 1:20,000, ab16645, Abcam, USA; GABA: rabbit anti-GABA, 1:1000; PA5-32241, Invitrogen, USA; Orexin: mouse anti-orexin-A,1:600, sc-80263, Santa Cruz Biotechnology, USA; MCH: rabbit anti-melanin-concentrating-hormone, 1:1000, M8440, Sigma-Aldrich, USA; Glutamate: rabbit anti-glutamate, 1:1000, G6642, Sigma, USA; and Biocytin: 1:1000, S21374, Invitrogen, USA) dissolved in PBST (0.3% Triton X-100 in PBS) overnight at 4 °C. The next day, slices were washed with PBS and incubated with secondary antibodies (Donkey anti-rabbit/goat, 1:1000; Jackson ImmunoResearch, USA) for 2 h. Fluorescence images were captured using a confocal microscope (Nikon AIR-MP).

Zhao Y.N., Jiang J.B., Tao S.Y., Zhang Y., Chen Z.K., Qu W.M., Huang Z.L, & Yang S.R. (2022). GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression. Nature Communications, 13, 7552.

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Characterization of Nanofibrous Scaffold Structure

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The morphology of the nanofibrous scaffolds was observed by SEM. Transmission electron microscopy (TEM; Tecnai G2 F20, FEI, USA) was employed to observe the nanofibers' core-shell structure. To further determine the core and shell layer in the coaxial structure fibers, traces of fluorescent agents were mixed with the electrospun solution (Coumarin-6 in the shell layer and Rhodamine B in the core layer). Confocal laser scanning microscopy (CLSM; AIR MP⁺, Nikon, Japan) was used to collect fluorescence images of the fibers. An electronic universal testing machine (AG-IC 50 KN, SHIMADZU, Japan) was applied to test the mechanical properties. All samples for mechanical properties tests were cut into a dumbbell shape. The scaffold's wettability was measured using a contact angle measuring instrument (JY-82 B, Chengde Dingsheng, China).

Jin S., Gao J., Yang R., Yuan C., Wang R., Zou Q., Zuo Y., Zhu M., Li Y., Man Y, & Li J. (2021). A baicalin-loaded coaxial nanofiber scaffold regulated inflammation and osteoclast differentiation for vascularized bone regeneration. Bioactive Materials, 8, 559-572.

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Cellular Uptake of CDs-PEI-AS1411 Nanoparticles

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Confocal laser scanning microscopy was employed to observe the cellular uptake of the CDs‐PEI‐AS1411. MCF‐7 and L929 cells were seeded in confocal microscope dishes at 2 × 10⁴ cells per well and incubated overnight. Then, the CDs‐PEI‐AS1411 were added to the above cells at the CDs concentration of 200 µg/mL. After different treatment time (6, 12, 24 and 48 hours), the supernatant was removed and the cells were fixed with 4% paraformaldehyde. Subsequently, the cell cytoskeleton was stained with FITC‐labelled phalloidin and the cells were imaged by a Nikon AIR‐MP confocal laser scanning microscope (Japan).

Kong T., Zhou R., Zhang Y., Hao L., Cai X, & Zhu B. (2019). AS1411 aptamer modified carbon dots via polyethylenimine‐assisted strategy for efficient targeted cancer cell imaging. Cell Proliferation, 53(1), e12713.

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Cellular Morphology Analysis of BMSCs

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To further investigate the impact of the diverse surface topological structure nanofiber matrix on BMSCs’ growth geometric, the cells cultured on the fibrous scaffolds were stained. First, 1 × 10⁴ BMSCs were cultured into every well in a 12-well plate, and the growth medium was discarded after incubation for 5 days. Second, 4% formaldehyde solution was added to fix the cells for 30 min and then they were cleaned with PBS to eliminate excessive formaldehyde. Third, cells’ F-actin and nucleus were labelled by Alexa Fluor^® 546 phalloidin and Hoechst 33342, respectively. In the last step, fluorescence images of the cellular morphology were obtained with a confocal laser scanning microscope (CLSM; AIR, MP+, Nikon, Tokyo, Japan). Image-Pro Plus software was used to quantify the cell nuclei orientation angle, nucleus area, and the cell nucleus aspect ratio. Mean values and standard deviations from 100 randomly chosen cells were calculated.

Zhang Y., Tang J., Fang W., Zhao Q., Lei X., Zhang J., Chen J., Li Y, & Zuo Y. (2023). Synergetic Effect of Electrical and Topographical Cues in Aniline Trimer-Based Polyurethane Fibrous Scaffolds on Tissue Regeneration. Journal of Functional Biomaterials, 14(4), 185.

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ICD Induction and CRT Exposure Analysis

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Immunofluorescent staining and an ATP assay kit were used to evaluate the ICD induced by mB4S. For observation of CRT exposure, 4T1 cells were plated in a confocal dish at a density of 3 × 10⁴ cells per dish for 12 h and treated with EPI, B4, B4S, and mB4S for 12 h at the same EPI concentration of 3 μg/mL. The treated 4T1 cells were washed with PBS twice and fixed in 4% paraformaldehyde for 15 min. Cells were blocked in a 5% BSA solution for 2 h and incubated with an anti-CRT antibody diluted at 1:5000 overnight at 4 °C and an APC-conjugated secondary antibody for 2 h. The cell nuclei were stained with DAPI for 8 min and washed gently twice with PBS for visualization via a confocal scanning laser microscope (CLSM, Nikon, AIRMP⁺, Tokyo, Japan, λ_ex = 633 nm, λ_em = 660 nm). To detect the ATP concentration in the cell culture supernatant, 4T1 cells were seeded in 6-well plates (5 × 10⁵ cells per well) and co-cultured with EPI, B4, B4S, and mB4S for 12 h. The supernatant was collected and centrifuged (800×g, 3 min) to remove cell debris, and the ATP content was detected according to the manufacturer's instructions.

Li Z., Zhang Q., Li Z., Ren L., Pan D., Gong Q., Gu Z., Cai H, & Luo K. (2024). Branched glycopolymer prodrug-derived nanoassembly combined with a STING agonist activates an immuno-supportive status to boost anti-PD-L1 antibody therapy. Acta Pharmaceutica Sinica. B, 14(5), 2194-2209.

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Whole-Brain Retrograde Tracing Analysis

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For whole-brain retrograde tracing, one out of every four sections was captured using a 20 × objective on a microscope (VS-120, Olympus, Tokyo, Japan). Other fluorescence images were captured using a confocal microscope (Nikon AIR-MP). Each slice was matched to the corresponding atlas level of the mouse brain atlas (Franklin and Paxinos, 2001 ). The dsRed-expressing neurons in individual nuclei within each whole brain were quantified semi-automatically using ImageJ software. First, the images were converted into 8-bit grayscale and adjusted using a threshold. Before automatic quantification with analysis of particles, cell size and circularity were set accordingly. Next, dsRed-labeled cells were manually checked. The proportion of input neurons from each of the 71 brain regions was calculated as the ratio of the total number of dsRed-labeled cells in each brain (including both sides with the injection site excluded). The neurons in each brain region, with a proportion of more than 0.1% of the total monosynaptic inputs, were calculated. Based on the proportion of dsRed-labeled cells in each nucleus, we defined the following four grades of afferent inputs: numerous inputs (over 6%), large inputs (3–6%), moderate inputs (1–3%), and few inputs (<1%), as shown in Figures 6, 7. All data are presented as mean ± standard error of the mean.

Zhao Y.N., Zhang Y., Tao S.Y., Huang Z.L., Qu W.M, & Yang S.R. (2022). Whole-Brain Monosynaptic Afferents to Rostromedial Tegmental Nucleus Gamma-Aminobutyric Acid-Releasing Neurons in Mice. Frontiers in Neuroscience, 16, 914300.

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Cellular Organelle Staining Protocol

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UM1 cells after incubation with S‐CDs were loaded with MitoTracker green (a mitochondria probe) and LysoTracker green (a lysosome probe), respectively. Samples were washed with PBS after 30 minutes incubation. Analysed photographs were taken from confocal laser scanning microscopy (AIR‐MP, Nikon).

Li Q., Zhou R., Xie Y., Li Y., Chen Y, & Cai X. (2020). Sulphur‐doped carbon dots as a highly efficient nano‐photodynamic agent against oral squamous cell carcinoma. Cell Proliferation, 53(4), e12786.

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Confocal Imaging of Neuronal Structures

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All images were acquired on a confocal microscope (Nikon, AIR-MP, Tokyo, Japan) with a 60× oil-immersion objective and at a resolution of 2048 × 2048 pixels using a z series of 15-20 images with a depth interval of 1 mm (0.5 mm for gephyrin-stained slices). A 10× air-immersion objective was then applied to obtain the images of brain slice regions captured before by the 60× objective with a depth interval of 5 μm to get the coordinates of PyNs (see "Image Analysis" for details).

Hu A., Zhao R., Ren B., Li Y., Lu J, & Tai Y. (2023). Projection-Specific Heterogeneity of the Axon Initial Segment of Pyramidal Neurons in the Prelimbic Cortex. Neuroscience bulletin, 39(7).

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Dual Immunohistochemistry for c-Fos and mCherry

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For dual immunostaining with c-Fos and mCherry, mice were deeply anesthetized with chloral hydrate (400 mg/kg) and were perfused with phosphate-buffered saline (PBS) followed by 4% PFA in 0.1-M phosphate buffer. The brain was then dissected and fixed in 4% PFA at 4°C overnight. Fixed samples were sectioned into 30-μm coronal sections using a freezing microtome (CM1950, Leica, Germany). For immunohistochemistry, the floating sections were washed in PBS and were then incubated in the following primary antibodies in PBS containing 0.3% Triton X-100 (PBST) at 4°C: anti-rabbit c-Fos (1:10000 for 48 h); primary antibody (Millipore); and anti-mouse NeuN (1:1000 for 12 h; MAB377, Millipore). Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally, the sections were mounted on glass slides, dried, dehydrated, and cover-slipped. Fluorescent images were collected with a confocal microscope (Nikon AIR-MP).

Chen C., Zhong Y., Jiang S., Xu W., Wang Z., Xiao L., Qu W., & Huang Z. (2021). Dysfunctions of the paraventricular hypothalamic nucleus induce hypersomnia in human and mice.

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Immunohistochemical Analyses of Mouse Brain

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Mice were anesthetized with chloral hydrate (intraperitoneal, 360 mg kg À1 ) and perfused transcardially with phosphate-buffered saline (PBS), followed by 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB). Brains were removed, post-fixed overnight, and incubated in PBS containing 20% sucrose until they sunk to the bottom. Coronal sections (30 mm) were cut on a microtome (Leica e2 Current Biology 29, 637-644.e1-e3, February 18, 2019 CM1900). The brain sections were washed five times for 3 min each in PBS and incubated with primary antibody in PBS containing 0.3% Triton X-100 (PBST) at 4 C for 24 h (anti-Fos, ABE457, 1:5,000, Millipore; anti-GABA, PA5-32241, 1:1,000, Thermo Fisher Scientific; anti-TH, AB152, 1:3,000, Millipore). Primary antibodies were washed five times with PBS before incubation with secondary antibodies (Alexa 594-conjugated goat anti-rabbit for Fos, GABA and TH, R37117, 1:1000, Thermo Fisher Scientific). Fluorescent images were collected with a confocal microscope (Nikon AIR-MP).

Zhang Z., Liu W.Y., Diao Y.P., Xu W., Zhong Y.H., Zhang J.Y., Lazarus M., Liu Y.Y., Qu W.M, & Huang Z.L. (2019). Superior Colliculus GABAergic Neurons Are Essential for Acute Dark Induction of Wakefulness in Mice. Current biology : CB, 29(4).

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