THP-1 cells were transfected separately with TLR4-siRNA (30 nM; OriGene Technologies, Inc. MD, USA), scramble (control) siRNA (30 nM; OriGene Technologies, Inc. MD, USA, USA) and pmaxGFP (0.5μg; Amaxa Noclecfector Kit V for THP-1, Lonza). Transfection experiments were carried out using Amaxa Electroporation System (Amaxa Inc., Germany), according to the manufacturer’s protocol [13 (link)]. Effective suppression of constitutive TLR4 expression in THP-1 cells transfected with specific gene-targeted siRNA was confirmed by real time RT-PCR.
Scramble control sirna
Manufactured by OriGene
Sourced in United States
The Scramble (control) siRNA is a laboratory tool used in RNA interference (RNAi) experiments. It serves as a negative control to help researchers evaluate the specificity of their target gene silencing experiments. The Scramble siRNA sequence does not target any known gene and is designed to have minimal impact on cellular processes, providing a baseline for comparison against specific gene knockdown effects.
Automatically generated - may contain errors
Lab products found in correlation
Amaxa noclecfector kit 5 for thp 1, by Lonza (4 mentions)
Amaxa cell line nucleofector kit 5 for monocytic cells, by Lonza (3 mentions)
Sirna acsl1, by OriGene (2 mentions)
Amaxa noclecfector kit 5, by OriGene (2 mentions)
Viromer blue, by BioNTech (2 mentions)
Amaxa electroporation system, by Lonza (2 mentions)
Tlr4 sirna, by OriGene (1 mentions)
Pmaxgfp, by Lonza (1 mentions)
Amaxa nucleofector kit 5, by Lonza (1 mentions)
Sirna cerk, by OriGene (1 mentions)
Amaxa noclecfector kit 5, by Santa Cruz Biotechnology (1 mentions)
Sirna n smase, by Santa Cruz Biotechnology (1 mentions)
5 protocols using scramble control sirna
1
Silencing TLR4 in THP-1 Cells
2
ACSL1 Knockdown in MDA-MB-231 Cells
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MDA-MB-231 cells were washed and re-suspended in 100 μL of nucleofector solution provided with the Amaxa Nucleofector Kit V and transfected separately with siRNA-ACSL1 (30 nM; OriGene Technologies, Inc., Rockville, MD, USA), scramble (control) siRNA (30 nM; OriGene Technologies, Inc., Rockville, MD, USA), and pmaxGFP (0.5 μg; Amaxa Nucleofector Kit V for MDA-MB-231 cells, Lonza, Basel, Switzerland). All transfection experiments were performed with an Amaxa Cell Line Nucleofector Kit V for MDA cells (Lonza, Basel, Switzerland) using an Amaxa Electroporation System (Lonza, Basel, Switzerland) according to the manufacturer’s protocol [37 (link)]. After 36 h of transfection, cells were treated with TNFα for 24 h. Cells were harvested for RNA isolation and staining study. ACSL1 gene knockdown level was assessed by real-time PCR using ACSL1 gene specific primer probes.
3
Monocyte Transfection and CERK Knockdown
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Monocytes were washed and resuspended in 100 ul of nucleofector solution provided with the Amaxa Noclecfector Kit V and transfected separately with siRNA-CERK (30nM; OriGene Technologies, Inc. MD, USA), scramble (control) siRNA (30nM; OriGene Technologies, Inc. MD, USA, USA), and pmaxGFP (0.5 ug; Amaxa Noclecfector Kit V for THP-1, Lonza). All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza, Germany) by using Amaxa
Electroporation System (Amaxa Inc, Germany) according to the manufacturer's protocol 16 . After 36 hours of transfection, cells were treated with TNF-α for 2 hours. Cells were transfected with 20 nM of the siRNA using Viromer Blue (lipocalyx, Halle, Germany) as per manufacture's instruction. Cells were harvested for RNA isolation and staining study. CERK gene knock down level was assessed by Real Time-PCR using CERK gene specific primer (CERK: Assay ID: Hs00368483; ThermoFisher Sceintific).
Electroporation System (Amaxa Inc, Germany) according to the manufacturer's protocol 16 . After 36 hours of transfection, cells were treated with TNF-α for 2 hours. Cells were transfected with 20 nM of the siRNA using Viromer Blue (lipocalyx, Halle, Germany) as per manufacture's instruction. Cells were harvested for RNA isolation and staining study. CERK gene knock down level was assessed by Real Time-PCR using CERK gene specific primer (CERK: Assay ID: Hs00368483; ThermoFisher Sceintific).
4
Monocyte Transfection and nSMase2 Knockdown
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Monocytes were washed and resuspended in 100 ul of nucleofector solution provided with the Amaxa Noclecfector Kit V and transfected separately with siRNA-n-SMase (Santa Cruz Biotechnology, INK. SC-106277), scramble (control) siRNA (30nM; OriGene Technologies, Inc. MD, USA, USA), and pmaxGFP (0.5 ug; Amaxa Noclecfector Kit V for THP-1, Lonza). All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza, Germany) by using Amaxa
Electroporation System (Amaxa Inc, Germany) according to the manufacturer's protocol (13) . After 36 hours of transfection, cells were treated with TNF-α for 2 hours. For knock down of nSMase2 in human primary monocyte cultures, cells were transfected with 20 nM of the siRNA using Viromer Blue (lipocalyx, Halle, Germany) according to the manufacturer's instructions. Cells were harvested for RNA isolation and staining study. nSMase gene knock down level was assessed by Real Time-PCR using SMPD3 genespecific primer probes.
Electroporation System (Amaxa Inc, Germany) according to the manufacturer's protocol (13) . After 36 hours of transfection, cells were treated with TNF-α for 2 hours. For knock down of nSMase2 in human primary monocyte cultures, cells were transfected with 20 nM of the siRNA using Viromer Blue (lipocalyx, Halle, Germany) according to the manufacturer's instructions. Cells were harvested for RNA isolation and staining study. nSMase gene knock down level was assessed by Real Time-PCR using SMPD3 genespecific primer probes.
5
Monocyte ACSL1 Gene Silencing
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Monocytes were washed and resuspended in 100 ul of nucleofector solution provided with the Amaxa Noclecfector Kit V and transfected separately with siRNA-ACSL1 (30nM; OriGene Technologies, Inc. MD, USA), scramble (control) siRNA (30nM; OriGene Technologies, Inc. MD, USA, USA), and pmaxGFP (0.5 ug; Amaxa Noclecfector Kit V for THP-1, Lonza). All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza, Germany) by using Amaxa Electroporation System (Amaxa Inc, Germany) according to the manufacturer's protocol [11] . After 36 hours of transfection, cells were treated with TNF-α for 6-12 hours. Cells were harvested for RNA isolation and staining study. ACSL1 gene knock down level was assessed by Real Time-PCR using ACSL1 gene specific primer probes.
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