Lambda 1050 uv vis nir spectrometer

Manufactured by PerkinElmer

Sourced in United States

The Lambda 1050 UV/Vis/NIR spectrometer is a high-performance analytical instrument designed for a wide range of spectroscopic applications. It features a dual-beam optical system and a wavelength range of 175 to 3,300 nanometers, covering the ultraviolet, visible, and near-infrared regions of the electromagnetic spectrum. The Lambda 1050 is capable of performing various spectroscopic measurements, such as absorbance, reflectance, and transmittance.

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17 protocols using lambda 1050 uv vis nir spectrometer

Characterizing Gold Nanoparticles by UV-Vis

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The formation of gold nanoparticles
was characterized by ultraviolet–visible spectroscopy (Perkin
Elmer UV/Vis/NIR Spectrometer Lambda 1050) using a wavelength window
of 200–800 nm in 10 mm-thick quartz cuvettes.

Abbas M., Susapto H.H, & Hauser C.A. (2022). Synthesis and Organization of Gold-Peptide Nanoparticles for Catalytic Activities. ACS Omega, 7(2), 2082-2090.

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Catalytic Reduction of p-Nitrophenol

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The catalytic reduction
of p-nitrophenol to p-aminophenol
by GPNP composites was conducted in a solution
containing 100 μL of 0.1 mM aqueous p-nitrophenol,
100 μL of 50-fold dilute GPNP composite from the initial stock
concentration of 2 mg/mL peptide and 0.72 mM gold concentration, and
100 μL of 0.1 M aqueous NaBH₄, which was freshly
prepared under ambient conditions. As a control, the reduction of p-nitrophenol was also conducted using a high concentration
of GPNPs (2 mg/mL IVFK and 0.72 mM gold concentration) with the same
ratio of p-nitrophenol and NaBH₄. Catalytic
performance was carried out inside a UV–Vis spectroscope (Perkin
Elmer UV/Vis/NIR Spectrometer Lambda 1050) to monitor the concentration
change of the reactant (i.e., p-nitrophenol) and
the product (i.e., p-aminophenol).

Abbas M., Susapto H.H, & Hauser C.A. (2022). Synthesis and Organization of Gold-Peptide Nanoparticles for Catalytic Activities. ACS Omega, 7(2), 2082-2090.

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Graphene Oxide Characterization and Separation

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Graphene oxide was prepared by using the Hummers method with modifications [34 , 35 (link)]. The individual graphite oxide flakes contain carboxyl groups mainly at the edges, and epoxide, hydroxide and ketone groups mainly on the basal plane. The C to O ratio is usually slightly lower or slightly higher than 1 as determined by X-ray photoemission spectroscopy. The graphene oxide flakes of different sizes were separated by centrifuging graphene oxide suspensions at various rpm and collecting different phases of the suspension. The AFM characterization of graphene oxide flakes was performed on a Bruker Dimension FastScan AFM system by using taping mode. The substrates were prepared by spin-casting the suspension on a Si/SiO2 substrate to yield monolayer film, followed by AFM imaging. Concentrations were obtained from UV-Vis spectra, which were recorded in 10 mm path length quartz cells using a PerkinElmer Lambda – 1050 UV-Vis-NIR spectrometer. The dispersions were diluted to give the absorption intensity lower than 1.

Fiorillo M., Verre A.F., Iliut M., Peiris-Pagés M., Ozsvari B., Gandara R., Cappello A.R., Sotgia F., Vijayaraghavan A, & Lisanti M.P. (2015). Graphene oxide selectively targets cancer stem cells, across multiple tumor types: Implications for non-toxic cancer treatment, via “differentiation-based nano-therapy”. Oncotarget, 6(6), 3553-3562.

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Spectroscopic Analysis of Aqueous Samples

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The NIR-UV-Vis measurements were carried out through a PerkinElmer lambda 1050 UV/Vis/NIR Spectrometer. Water absorbance spectrum was measured under 150 mm InGaAs Int. Sphere Absorbance module and the rest were carried out through 3D WB Det. Absorbance Module. Before each measurement, a 100% transmittance (0 absorbance) baseline was auto-zeroed. The water spectrum was denoised through white certified reflectance standard from Labsphere Company while the rest background was calibrated with pure water. The detection cuvette had a transmittance length of 5 mm. The injected beam (Slit width of 2.00 nm) was sourced from the combination of D2 Lamp and Tungsten Lamp with a lamp change at 860.8 nm. The spectra were collected in the wavelength range from 1000 nm to 700 nm with a data interval of 1 nm.

Gao X., Chen X., Hu H., Wang X., Yue W., Mu J., Lou Z., Zhang R., Shi K., Chen X., Lin M., Qi B., Zhou S., Lu C., Gu Y., Yang X., Ding H., Zhu Y., Huang H., Ma Y., Li M., Mishra A., Wang J, & Xu S. (2022). A photoacoustic patch for three-dimensional imaging of hemoglobin and core temperature. Nature Communications, 13, 7757.

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MXene Gel Inks for Anti-Counterfeiting

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A LAMBDA 1050 + UV–VIS–NIR spectrometer (PerkinElmer, USA) equipped with an integrating sphere was used to measure the NIR emissivity of MXene gel inks at pH 4 and 10. In a typical screen-printing process, a certain amount of gel inks was spread onto a PET substrate that have been coated, cured and washed previously. Afterward, the inks were rapidly scraped across the patterns by a scraper. Alternatively, the inks can be directly extrusion-printed into different passwords or patterns on the substrate via a syringe. The thickness of the gel coatings can be regulated by multiple times printing. After natural drying, the anticounterfeiting performance was analyzed either photoemissively by heating the gel films with a HP-E1515 hot plate (Hanbang electronics, China) at 50 °C or photothermally by illuminating the ink coatings with an 808-nm FC-11 NIR laser (Changchun New Industries Optoelectronics Technology, China) at 3 W cm⁻². A UTi120S thermal imager (Yuride, China) was used to detect the surface temperature and IR images of the screen- or extrusion-printed patterns.

Yu W., Yang Y., Wang Y., Hu L., Hao J., Xu L, & Liu W. (2024). Versatile MXene Gels Assisted by Brief and Low-Strength Centrifugation. Nano-Micro Letters, 16, 94.

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Spectroscopic Characterization of Nanomaterials

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UV-Vis absorption measurements were performed on a PerkinElmer Lambda 1050 UV/Vis/NIR spectrometer (PerkinElmer, Santa Clara, CA), equipped with a Peltier temperature control accessory. Zeta potential of nanomaterials was measured using Zetasizer nano (Malvern Panalytical, UK). Fluorescence spectra were obtained with an RF-6000 spectrophotometer (Shimadzu, Japan). Transmission electron microscopy (TEM) images of GO and AuNPs were taken using a Hitachi 7500 transmission electron microscope (Hitachi, Japan). All ICP-MS measurements were carried out using a Thermo Scientific iCAP Qc ICP-MS (Bremen, Germany) coupled with a 4-channel 12-roller peristaltic pump, nickel sample and skimmer cones, a Teledyne CETAC ASX560 autosampler (Omaha, NE), a microflow perfluoroalkoxy nebulizer (Thermo Scientific) and a Peltier-cooled quartz cyclonic spray chamber. To monitor the ICP-MS instrument, the THERMO-4AREV (Thermo Scientific) standard was run daily for maximum ⁵⁹Co, ²³⁸U and minimum ¹⁴⁰Ce¹⁶O/¹⁴⁰Ce signal. The ICP-MS measurements of AuNPs were controlled by the Qtegra™ software (version 2.8.2944.202). The instrument operating parameters used for the single particle and conventional ICP-MS measurements are listed in Table S1 (Supporting Information).

Xing Y., Han J., Wu X., Pierce D.T, & Zhao J.X. (2021). Graphene/Gold Nanoparticles Composites for Ultrasensitive and Versatile Biomarker Assay Using Single-Particle Inductively-Coupled Plasma/Mass Spectrometry. The Analyst, 145(24), 7932-7940.

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Polymer Characterization by Comprehensive Spectroscopic Analysis

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All compounds were characterized by ¹H NMR (300 MHz, Bruker) and ¹³C NMR (500 MHz, Jeol) using CDCl₃ as the solvent. The residual chloroform peak at 7.28 ppm was used to calibrate the chemical shifts for ¹H NMR. Gel-permeation chromatography (GPC) was carried out in chloroform using an Agilent 1260 separation module equipped with a 1260 refractive index detector and a 1260 photodiode array detector. Molecular weights were calculated relative to linear polystyrene standards. Atomic force micrographs were obtained using a Veeco scanning probe microscope (SPM) in tapping mode, and the data were analyzed with NanoScope Analysis v1.40 software (Bruker Corp.). Ultraviolet-visible (UV-vis) absorption spectra were obtained for polymers in chloroform and in the solid state (as cast from chloroform at 10 mg mL^–1) using a Perkin Elmer Lambda 1050 UV-vis-NIR spectrometer.

Sugiyama F., Kleinschmidt A.T., Kayser L.V., Rodriquez D., Finn M I.I.I., Alkhadra M.A., Wan J.M., Ramírez J., Chiang A.S., Root S.E., Savagatrup S, & Lipomi D.J. (2018). Effects of flexibility and branching of side chains on the mechanical properties of low-bandgap conjugated polymers. Polymer chemistry, 9(33), 4354-4363.

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UV/Vis Spectroscopy of Drug-Loaded Particles

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The UV/Vis measurements were performed on a Lambda 1050 UV/Vis/NIR spectrometer form Perkin Elmer (Waltham, MA, USA). The software used to record the measured spectra was Perkin Elmer UVWinLab (Waltham, MA, USA). For the measurements, the loaded coated particles were dissolved in ALF to facilitate the release of all their cargo. 1 mL of this solution was then diluted with 2 mL H₂O yielding a total of 3 mL and measured in a quartz cuvette. For the pure drugs, 1 mL of the aqueous solution was diluted with 1 mL H₂O and 1 mL ALF.

Illes B., Wuttke S, & Engelke H. (2017). Liposome-Coated Iron Fumarate Metal-Organic Framework Nanoparticles for Combination Therapy. Nanomaterials, 7(11), 351.

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Characterization of PMPSi Polymer Films

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PMPSi was obtained from Fluorochem Ltd. UK, GPC analysis revealed Mw = 27,600 g/mol and Mn = 8500 g/mol. Films for UV–Vis measurements were prepared by the spin coating method using spin coater Laurell WS-650-MZ-23NPP from the solution in toluene. Quartz glass was used as a substrate. The absorption spectrum was measured by Lambda 1050 UV/Vis/NIR spectrometer from Perkin Elmer.

Hanulikova B., Kuritka I, & Urbanek P. (2016). Effect of backbone conformation and its defects on electronic properties and assessment of the stabilizing role of π–π interactions in aryl substituted polysilylenes studied by DFT on deca[methyl(phenyl)silylene]s. Chemistry Central Journal, 10, 28.

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Organic Photoluminescence Characterization

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Solution samples were prepared with toluene at a concentration of 0.01 mM. The neat film was deposited on quartz at a rate of 1 angstrom per second under vacuum. The UV-Vis absorption spectrum was obtained using a Lambda 1050 UV/Vis/NIR spectrometer (PerkinElmer). Photoluminescence (PL) spectra were recorded using the Photon Technology International QM-40. Absolute photoluminescence quantum yield (PLQY) and transient quantaurus-QY (Hamamatsu) were obtained. The low-temperature photoluminescence spectrum was measured at 77 K using the Jasco FP-6500. Photoluminescent decay traces were obtained through time-correlated single-photon coefficient (TCSPC) technology using PicoQuant, FluoTime 250 instruments (PicoQuant, Germany). A 377 nm pulse laser was used as an excitation source and data analysis was performed using the exponential fitting model of the FluoFit software.

Park D., Kang S., Ryoo C.H., Jhun B.H., Jung S., Le T.N., Suh M.C., Lee J., Jun M.E., Chu C., Park J, & Park S.Y. (2023). High-performance blue OLED using multiresonance thermally activated delayed fluorescence host materials containing silicon atoms. Nature Communications, 14, 5589.

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