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2 protocols using igd 11 26c fitc

1

Multiparameter Flow Cytometry Analysis

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Single cell suspensions from tissues were ACK lysed and stained in 1% FBS in PBS containing 0.05% sodium azide. Cells were gated according to size and granularity based on FSC-A and SSC-A. Doublets were excluded by FSC-A versus FSC-H gating. Non-antigen-specific binding was blocked with CD16/CD32 (2.4G2) (BD Biosciences). The following antibodies were used for staining: B220 (RA3–6B2) APC-eFluor780, IgM (II/41) APC, IgD (11–26c) FITC, CD86 (PO3.1) PE, hCD2 (RPA-2.10) APC (Thermo Fisher Scientific), CXCR4 (L276F12) APC, CD86 (GL1) PerCP Cy5.5 (BioLegend), κ (187.1) FITC, CD19 (1D3) APC-Cy7, FAS (Jo2) PE-Cy7, GL7 FITC and IgG1 (A85–1) FITC (BD Biosciences). Cell proliferation was analyzed using the eBioscience Cell Proliferation Dye eFluor670 (Thermo Fisher Scientific). Samples were acquired on a FACSCanto (BD Biosciences) and analyzed with Flowjo (Becton, Dickinson and Company).
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions from tissues were ACK lysed and stained in 1% FBS in PBS containing 0.05% sodium azide. Cells were gated according to size and granularity based on FSC-A and SSC-A. Doublets were excluded by FSC-A versus FSC-H gating. Non-antigen-specific binding was blocked with CD16/CD32 (2.4G2) (BD Biosciences). The following antibodies were used for staining: B220 (RA3–6B2) APC-eFluor780, IgM (II/41) APC, IgD (11–26c) FITC, CD86 (PO3.1) PE, hCD2 (RPA-2.10) APC (Thermo Fisher Scientific), CXCR4 (L276F12) APC, CD86 (GL1) PerCP Cy5.5 (BioLegend), κ (187.1) FITC, CD19 (1D3) APC-Cy7, FAS (Jo2) PE-Cy7, GL7 FITC and IgG1 (A85–1) FITC (BD Biosciences). Cell proliferation was analyzed using the eBioscience Cell Proliferation Dye eFluor670 (Thermo Fisher Scientific). Samples were acquired on a FACSCanto (BD Biosciences) and analyzed with Flowjo (Becton, Dickinson and Company).
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