Anti mouse ctla 4 uc10 4b9

Manufactured by Thermo Fisher Scientific

Sourced in Germany

Anti-mouse CTLA-4 (UC10-4B9) is a laboratory reagent used for the detection of mouse CTLA-4 protein. It is a monoclonal antibody that specifically binds to the CTLA-4 (Cytotoxic T-Lymphocyte Associated Protein 4) antigen expressed on mouse cells.

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Lab products found in correlation

Anti mouse foxp3 fjk 16s, by Thermo Fisher Scientific (3 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (2 mentions) Anti mouse cd45 30 f11, by BioLegend (1 mentions) Anti mouse cd25 pc61, by BioLegend (1 mentions) Anti mouse tcrβ h57 597, by Thermo Fisher Scientific (1 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Anti mouse cd4 gk1, by BD (1 mentions) Anti mouse cd11c n418, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by BD (1 mentions) Anti mouse cd8 53 6, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CTLA-4 (19 citations) Anti-CTLA-4 (7 citations) CTLA-4 (UC10-4B9), (5 citations) (UC10-4B9, (3 citations) Anti-CTLA-4 (UC10-4B9). (2 citations)

3 protocols using anti mouse ctla 4 uc10 4b9

Comprehensive Immune Cell Profiling

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The following monoclonal antibodies were used for cell surface staining: anti-mouse CD45 (30-F11; BioLegend), anti-mouse TCRβ (H57-597; eBioscience), anti-mouse TCRγδ (eBioGL3 (GL-3, GL3); eBioscience), anti-mouse Vγ1 (2.11; BioLegend), anti-mouse CD4 (RM4-5; BioLegend), anti-mouse CD25 (PC61; BioLegend), anti-mouse CD27 (LG.3A10; BD Biosciences), anti-mouse CD39 (Duha59; BioLegend), anti-mouse CD44 (IM7; BioLegend), anti-mouse CD62L (MEL-14; eBioscience). The following antibodies were used for intracellular staining: anti-mouse Foxp3 (FJK-16s; eBioscience), anti-RORγt (Q31-415 378; BD Biosciences), anti-mouse IL-17A (eBio17B7; eBioscience), anti-mouse IFN-γ (XMG1.2; eBioscience), anti-mouse CTLA-4 (UC10-4B9; eBioscience).

Lee W.S., Nam K.H., Kim J.H., Kim W.J., Kim J.E., Shin E.C., Kim G.R, & Choi J.M. (2023). Alleviating psoriatic skin inflammation through augmentation of Treg cells via CTLA-4 signaling peptide. Frontiers in Immunology, 14, 1233514.

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Comprehensive Immune Cell Profiling

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Lung cells were stained with anti-mouse CD45 Pacific Blue (30-F11) for determination of leukocytes in the lung tissue. Splenocytes, cLN cells, and lung cells were further fluorochrome-labeled with anti-mouse CD3ϵ (145-2C11), CD4 (RM4-5), CD8 (53-6.7), CD45R/B220 (RA3-6B2), CD39 (24DMS1), CD73 (TY/11.8) and CD103 (M290). Detection of FoxP3 and CTLA-4 was performed using the FoxP3 staining kit from eBioscience (Frankfurt, Germany) with anti-mouse FoxP3 (FJK-16 s) and anti-mouse CTLA-4 (UC10-4B9), according to the manufacturer’s recommendations. All antibodies used in this study, were obtained from BD Biosciences (Heidelberg, Germany), BioLegend (Fell, Germany) or eBioscience (Frankfurt, Germany).

Wirsdörfer F., Cappuccini F., Niazman M., de Leve S., Westendorf A.M., Lüdemann L., Stuschke M, & Jendrossek V. (2014). Thorax irradiation triggers a local and systemic accumulation of immunosuppressive CD4+ FoxP3+ regulatory T cells. Radiation Oncology (London, England), 9, 98.

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Comprehensive Immunophenotyping of Immune Cells

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Fluorochrome-conjugated monoclonal/polyclonal antibodies used in our studies were anti-mouse CD4 (GK1.5, BD Biosciences), anti-mouse CD25 (PC61.5l, eBioscience), anti-mouse Foxp3 (FJK-16s, eBioscience), anti-mouse CD8 (53-6.7, eBioscience), anti-human CD19 (H1B19, eBioscience), anti-human EGFR (AY13, BioLegend), anti-mouse CD45.1 (A20, eBioscience), anti-mouse CTLA-4 (UC10-4B9, eBioscience), anti-mouse neuropilin 1 (eDS304M, eBioscience), anti-mouse Lag-3 (C9B7W, eBioscience), anti-mouse CD11c (N418, eBioscience), anti-mouse IFN-γ (XMG1.2, eBioscience), anti-mouse TNF-α (MP6-XT22, eBioscience), anti-mouse Fas (SA367H8, BioLegend), anti-mouse FasL (MFL3, eBioscience), anti-mouse perforin (eBioMAK-D, eBioscience), anti-mouse GzB (NGZB, eBioscience), anti-mouse GzA (3G8.5, BioLegend), anti-mouse CD107α (1D4B, eBioscience), anti-mouse CD71 (R17217, eBioscience), anti-mouse CPT1a (8F6AE9, Abcam), anti-mouse Glut1 (ER3915, Abcam), and Fixable viability dye eFluor 780 (BD Biosciences). Intracellular staining was performed using the fixation/permeabilization concentrate (catalog 5123-43) and diluent (catalog 5223-56) buffer solutions and IC buffer (catalog 8333-56), according to the eBioscience Foxp3 staining kit. Stained cells were analyzed on an LSR Fortessa flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).

Bolivar-Wagers S., Loschi M.L., Jin S., Thangavelu G., Larson J.H., McDonald-Hyman C.S., Aguilar E.G., Saha A., Koehn B.H., Hefazi M., Osborn M.J., Jensen M.C., Wagner J.E., Pennell C.A, & Blazar B.R. (2022). Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses. JCI Insight, 7(17), e160674.

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