Ex wax paraffin embedded dna extraction kit

Manufactured by Merck Group

The EX-WAX™ Paraffin-embedded DNA Extraction Kit is a laboratory equipment product designed for the extraction of DNA from paraffin-embedded tissue samples. The kit provides a reliable and efficient method for isolating genomic DNA from these types of samples.

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Lab products found in correlation

Human methylation 450k beadchip, by Illumina (3 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (3 mentions) Dneasy blood tissue kit, by Qiagen (3 mentions) Ez dna methylation gold kit, by Zymo Research (3 mentions) Restoration kit, by Illumina (1 mentions)

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Paraffin (215 citations) Paraffin wax (78 citations) DNA extraction kit (17 citations)

3 protocols using ex wax paraffin embedded dna extraction kit

Genome-wide DNA Methylation Analysis

Cited 2 times

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For genome-wide DNA methylation analysis DNA was isolated from macro-dissected tumor tissue (frozen samples: DNeasy Blood & Tissue Kit, Qiagen; formalin fixed paraffin embedded (FFPE) samples: EX-WAX™ Paraffin-embedded DNA Extraction Kit, S4530; Merck KGAa) and quantified (Quant-iT™ PicoGreen® dsDNA Assay Kit, #P7589, Life Technologies). DNA samples were analyzed on the Human Methylation 450 K BeadChip (Illumina, San Diego CA, USA) at the Genomics platform of the University of Geneva. FFPE-derived tumor DNA samples were processed after passing a PCR-based quality control (Infinium HD FFPE QC Assay Protocol). DNA samples were subjected to bisulfite treatment (EZ DNA Methylation-Gold™ Kit, Zymo Research) as previously described, and FFPE samples were analyzed in separate batches after pretreatment with the restauration kit as recommended (llumina) [3 (link), 26 (link)].

Bady P., Marosi C., Weller M., Grønberg B.H., Schultz H., Taphoorn M.J., Gijtenbeek J.M., van den Bent M.J., von Deimling A., Stupp R., Malmström A, & Hegi M.E. (2022). DNA methylation-based age acceleration observed in IDH wild-type glioblastoma is associated with better outcome—including in elderly patients. Acta Neuropathologica Communications, 10, 39.

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Genome-Wide DNA Methylation Profiling

Cited 1 time

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For genome-wide DNA methylation analysis, DNA was isolated from macro-dissected tumor tissue (frozen samples: DNeasy Blood & Tissue Kit, Qiagen; FFPE samples: EX-WAX™ Paraffin-embedded DNA Extraction Kit, S4530; Merck KGAa) and quantified (Quant-iT™ PicoGreen^® dsDNA Assay Kit, #P7589, Life Technologies). Tumor DNA samples of 150 patients passing a PCR-based quality control (Infinium HD FFPE QC Assay Protocol) were subjected to bisulfite treatment (EZ DNA Methylation-Gold™ Kit, Zymo Research) as previously described [27 (link)], and were analyzed on the Human Methylation 450K BeadChip (Illumina, San Diego CA, USA) according to the manufacturer’s protocol at the Genomics platform of the University of Geneva. FFPE samples were analyzed in separate batches after pretreatment with the restoration kit as recommended (Illumina). The dataset is available under the accession number GSE104293 at GEO (http://www.ncbi.nlm.nih.gov/geo/).

Bady P., Kurscheid S., Delorenzi M., Gorlia T., van den Bent M.J., Hoang-Xuan K., Vauléon É., Gijtenbeek A., Enting R., Thiessen B., Chinot O., Dhermain F., Brandes A.A., Reijneveld J.C., Marosi C., Taphoorn M.J., Wick W., von Deimling A., French P., Stupp R., Baumert B.G, & Hegi M.E. (2018). The DNA methylome of DDR genes and benefit from RT or TMZ in IDH mutant low-grade glioma treated in EORTC 22033. Acta Neuropathologica, 135(4), 601-615.

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Genome-Wide DNA Methylation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For genome-wide DNA methylation analysis DNA was isolated from macro-dissected tumor tissue (frozen samples: DNeasy Blood & Tissue Kit, Qiagen; formalin fixed paraffin embedded (FFPE) samples: EX-WAX™ Paraffin-embedded DNA Extraction Kit, S4530; Merck KGAa) and quantified (Quant-iT™ PicoGreen® dsDNA Assay Kit, #P7589, Life Technologies). DNA samples were analyzed on the Human Methylation 450K BeadChip (Illumina, San Diego CA, USA) at the Genomics platform of the University of Geneva. FFPE-derived tumor DNA samples were processed after passing a PCR-based quality control (Infinium HD FFPE QC Assay Protocol). DNA samples were subjected to bisulfite treatment (EZ DNA Methylation-Gold™ Kit, Zymo Research) as previously described, and FFPE samples were analyzed in separate batches after pretreatment with the restauration kit as recommended (llumina) [3, 24] (link).

Bady P., Marosi C., Weller M., Grønberg B.H., Schultz H., Taphoorn M.J., Gijtenbeek J.M.M., Bent M.J.v.d., Deimling A.v., Stupp R., Malmström A., & Hegi M.E. (2022). DNA methylation-based age acceleration observed in IDH wild-type glioblastoma is associated with better outcome - including in elderly patients.

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