Subjects were recruited as part of the San Diego Acute and Early Infectious Disease Research Program and all subjects signed informed consents to protocols approved by the University of California San Diego Human Subjects Committee. Plasma samples were collected from 10 acutely infected chronic subjects (progressors) and 9 spontaneous controllers at 4, 12, 24 and 48 weeks after the estimated date of infection[27 (link)].
Controllers and progressors were defined using previously established criteria[28 (link)]. Specifically, controllers included individuals who maintained viral loads at or below 3000 copies/ml for at least 3 visits over the first year of infection in the absence of antiretroviral therapy. Median plasma viral loads for controllers were 392 (week 4), 206 (week 12), 558 (week 24) and 733 (week 48). Median plasma viral loads for progressors were 319,182 (week 4), 133,000 (week 12), 106,000 (week 24) and 80,950 (week 48). Controllers had significantly lower median plasma viral loads at all 4 time points.
IgG was purified from plasma samples using Melon Gel (Thermo Scientific). Total IgG concentration was calculated by Human IgG ELISA kit (MABTECH). All assays were repeated in triplicate.
Controllers and progressors were defined using previously established criteria[28 (link)]. Specifically, controllers included individuals who maintained viral loads at or below 3000 copies/ml for at least 3 visits over the first year of infection in the absence of antiretroviral therapy. Median plasma viral loads for controllers were 392 (week 4), 206 (week 12), 558 (week 24) and 733 (week 48). Median plasma viral loads for progressors were 319,182 (week 4), 133,000 (week 12), 106,000 (week 24) and 80,950 (week 48). Controllers had significantly lower median plasma viral loads at all 4 time points.
IgG was purified from plasma samples using Melon Gel (Thermo Scientific). Total IgG concentration was calculated by Human IgG ELISA kit (MABTECH). All assays were repeated in triplicate.