2-thiobarbituric acid and EDTA-Na2 were obtained from Merck KGaA (Darmstadt, Germany), absolute ethanol and n-butanol were purchased from Chimopar (Bucharest, Romania). o-Phthaldehyde and Bradford reagent were obtained from Sigma–Aldrich Chemicals GmbH (Germany). Antibodies against HDAC1 (Cat# sc-56683, RRID : AB_783697), MeCP2 ( Cat# sc-5755, RRID : AB_648930), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and secondary HRP-linked antibodies were purchased from Santa Cruz Biotechnology, Heidelberg, Germany. The test for quantification of phospho-NFkB p65 (Ser536) InstantOne™ ELISA was bought from Blue Gene, China. Glucose levels were measured by using a kit supplied by Diagnosticum Rt (Hungary). Chlorogenic acid, p-coumaric acid, caffeic acid, rosmarinic acid, rutin, apigenin, quercetin, isoquercitrin, quercitrin, hyperoside, kaempferol, myricetin, fisetin were purchased from Sigma Aldrich (St. Louis, USA). Ferulic acid, sinapic acid, gentisic acid, gallic acid, patuletin, luteolin from Roth (Karlsruhe, Germany) and cichoric acid, caftaric acid were obtained from Dalton (Toronto, Canada). HPLC grade methanol, ethanol and all reagents for spectrophotometric assays were purchased from Merck (Germany). All chemicals and reagents were of high-grade purity.
Gentisic acid
Manufactured by Carl Roth
Sourced in Germany
Gentisic acid is a chemical compound used as a laboratory reagent. It is a white, crystalline powder that is soluble in water and organic solvents. Gentisic acid is commonly used in various analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Luteolin, by Carl Roth (4 mentions)
Patuletin, by Carl Roth (4 mentions)
Ferulic acid, by Carl Roth (4 mentions)
Sinapic acid, by Carl Roth (4 mentions)
Apigenin, by Merck Group (4 mentions)
Hyperoside, by Merck Group (4 mentions)
Fisetin, by Merck Group (4 mentions)
Quercetin, by Merck Group (4 mentions)
Chlorogenic acid, by Merck Group (4 mentions)
Kaempferol, by Merck Group (4 mentions)
P coumaric acid, by Merck Group (4 mentions)
Caffeic acid, by Merck Group (4 mentions)
Rutin, by Merck Group (4 mentions)
Isoquercitrin, by Merck Group (4 mentions)
Quercitrin, by Merck Group (3 mentions)
Myricetin, by Merck Group (3 mentions)
Gallic acid, by Carl Roth (2 mentions)
Rosmarinic acid, by Merck Group (2 mentions)
Edta na2, by Merck Group (1 mentions)
2 thiobarbituric acid, by Merck Group (1 mentions)
4 protocols using gentisic acid
1
Quantification of Oxidative Stress Markers and Signaling Pathways
2
Quantification of Polyphenols in Propolis and Honey
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Polyphenolic compounds in hydroalcoholic propolis extract, oily propolis extract and honey sample were quantified using a previously described HPLC-UV-MS method [35 (link)]. Eighteen external standards were used: caffeic acid, chlorogenic acid, p-coumaric acid, kaempferol, apigenin, rutin, quercetin, quercitrin, isoquercitrin, fisetin, hyperoside, myricetin (Sigma, Neustadt, Germany), ferulic acid, gentisic acid, sinapic acid, patuletin, luteolin (Roth, Germany) and caftaric acid (Dalton, Toronto, ON, Canada). Calibration curves in the 0.5–50 μg/mL range with good linearity (R2 > 0.999) were used to determine the concentration of polyphenols in the sample.
3
Analytical Profiling of Phytochemicals
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High purity chemicals: sodium carbonate, sodium acetate trihydrate, and anhydrous aluminum chloride were acquired from Sigma-Aldrich (Germany). Folin-Ciocâlteu reagent was purchased from Merck (Germany). The standard chemicals; chlorogenic acid, p-coumaric acid, caffeic acid, rutin, apigenin, quercetin, isoquercitrin, hyperoside, kaempferol, quercetol, myricetol, fisetin, gallic acid, aucubin, catalpol, and harpagoside were sourced from Sigma-Aldrich (Germany), ferulic acid, sinapic acid, gentisic acid, patuletin, luteolin from Roth (Germany), caftaric acid from Dalton (United States), harpagide, and 8-O-acetyl-harpagide from PhytoLab GmbH & Co. (Germany). HPLC grade solvents (methanol, acetonitrile, ammonium acetate, and silver nitrate) were purchased from Sigma-Aldrich. Distilled, deionised water was produced by a Direct Q-5 Millipore (Millipore SA, Molsheim, France) water system.
4
Phytochemical Profiling of Burkina Faso Bark
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The raw material was represented by the barks of 4 species of plants harvested in April 2019 from the Bobo-Dioulasso region, Burkina Faso (11°13′21″ north, 4°25′37″ west). The species were identified by Doctor of Botany and cytotechnologist, Yempabou Hermann Ouoba, from the Nazi Boni University of Bobo, Dioulasso (UNB). The collected bark was dried at room temperature and then powdered using a Retsch Knife Mill Grindomix GM 200 (RETSCH GmbH, Eragny, France). The studied species were Parkia biglobosa (Jacq.) R.Br. ex G.Don (Fabaceae/Mimosaceae); Detarium microcarpum Guill. & Perr. (Fabaceae); Vitellaria paradoxa C.F.Gaertn. (Sapotaceae); and Sclerocarya birrea (A. Rich.) Hochst (Anacardiaceae).
Some HPLC references, such as chlorogenic acid, p-coumaric acid, caffeic acid, rosmarinic acid, rutin, apigenin, quercetin, isoquercitrin, quercitrin, hyperoside, kaempferol, myricetin, and fisetin were purchased from Sigma Aldrich (St. Louis, MO, USA). Cichoric acid, caftaric acid, ferulic acid, sinapic acid, gentisic acid, gallic acid, patuletin, and luteolin were obtained from Roth (Karlsruhe, Germany). HPLC grade solvents were purchased from Merck (Darmstadt, Germany). All chemicals and reagents were of high-grade purity.
λ-carrageenan and diclofenac were purchased from Sigma Aldrich, St. Louis, MO, USA.
Some HPLC references, such as chlorogenic acid, p-coumaric acid, caffeic acid, rosmarinic acid, rutin, apigenin, quercetin, isoquercitrin, quercitrin, hyperoside, kaempferol, myricetin, and fisetin were purchased from Sigma Aldrich (St. Louis, MO, USA). Cichoric acid, caftaric acid, ferulic acid, sinapic acid, gentisic acid, gallic acid, patuletin, and luteolin were obtained from Roth (Karlsruhe, Germany). HPLC grade solvents were purchased from Merck (Darmstadt, Germany). All chemicals and reagents were of high-grade purity.
λ-carrageenan and diclofenac were purchased from Sigma Aldrich, St. Louis, MO, USA.
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